Beneficial Effects of Magnesium Treatment on Heart Rate Variability and Cardiac Ventricular Function in Diabetic Rats

Amoni, Matthew; Kelly-Laubscher, Roisin; Blackhurst, Dee; Gwanyanya, Asfree

doi:10.1177/1074248416653831

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…It has been previously shown that the administration of magnesium as a supplement in rats in the early phase of diabetes leads to improved compliance of heart ventricles, as well as to the normalisation of the autonomous system of the myocardium, while the long-term effects of magnesium supplementation and their mechanisms remain unknown. 22 The aim of this study was to ascertain the effect of subchronic intake of magnesium hydrocarbonate -rich mineral water, as a form of magnesium supplementation, on cardiometabolic markers and electrolyte levels in rats with streptozotocininduced diabetes.…”

Section: Methodsmentioning

confidence: 99%

The effects of subchronic intake of magnesium hydro-carbonate-rich mineral water on cardiometabolic markers and electrolytes in rats with streptozotocin-induced diabetes

Đuric¹,

Gatarić²,

Todorović³

et al. 2022

Scripta Medica

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Background/Aim: Hypomagnesaemia is one of the most detected electrolyte abnormalities in diabetics. Modulation of numerous cardiovascular pathophysiological processes is a potential goal for anti-diabetic therapy. Magnesium supplementation prevents subclinical tissue magnesium deficiency, thus delaying the onset of metabolic imbalance in diabetes, but long-term effects of magnesium supplementation in chronic diabetes and numerous pathophysiological processes remain unknown. Aim of this study was to determine the effects of subchronic intake of magnesium hydrocarbonate-rich mineral water on cardiometabolic markers and electrolytes in rats with streptozotocin-induced diabetes. Methods: A total of 28 Wistar, male rats, body weight 160 g at start, were divided into four groups of 7 each: two controls, group that drank tap water and received a single ip injection of saline (0.9 % NaCl) (TW-C), group that drank mineral water rich in magnesium hydrocarbonate and received a single ip injection of saline (0.9 % NaCl) (MW-C); and two experimental groups with streptozotocin-induced diabetes, group that drank tap water and received a single ip injection of streptozotocin (100 mg/kg) in saline (0.9 % NaCl, 1 mL) (TW-DM), group that drank mineral water rich in magnesium hydrocarbonate and received a single ip injection of streptozotocin (100 mg/kg) in saline (0.9 % NaCl, 1 mL) (MW-DM). Results: Regarding the biochemical parameters, a decrease was observed in the MW-C group for vitamin B12 and proteins, while triglycerides were higher compared to the TW-C group. By comparing the haemostatic biomarkers between TW-C and MW-C groups, a statistically significant decrease was found for fibrinogen, while the electrolyte analysis showed an increase in phosphates for the MW-C group. Biochemical value comparison between TW-DM and MWDM groups showed that magnesium hydrocarbonate usage in diabetic rats did not significantly reduce glycaemia although the average glycaemic values were lower in the group treated with magnesium hydrocarbonate. Regarding the electrolyte values, a statistically significant decrease was observed for sodium, potassium and phosphate in the MW-DM group. The MW-DM group also showed a significant increase in iron value compared to TW-DM group. Conclusion: Subchronic intake of magnesium hydrocarbonate-rich mineral water, as a form of magnesium supplementation, did not cause a significant improvement in glycaemia or normalisation of diabetes-induced dyslipidaemia. This study showed the reduction of fibrinogen value, thus indicating the possibility of usage of this form of magnesium supplementation in different pro-thrombogenic conditions.

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Section: Methodsmentioning

confidence: 99%

The effects of subchronic intake of magnesium hydro-carbonate-rich mineral water on cardiometabolic markers and electrolytes in rats with streptozotocin-induced diabetes

Đuric¹,

Gatarić²,

Todorović³

et al. 2022

Scripta Medica

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“…Blood used for Mg 2+ measurements was collected at the time of excision of the heart (24 hours after the final MgSO 4 or saline injection). The blood was centrifuged to obtain plasma, from which ionized Mg 2+ concentration was measured using photometric assays (Beckman AU, PathCare, SA) as previously described (Amoni et al 2017a).…”

Section: Plasma Mg 2+ Measurementsmentioning

confidence: 99%

Cardioprotective effect of fingolimod against calcium paradox–induced myocardial injury in the isolated rat heart

Alatrag

Amoni

Kelly-Laubscher

et al. 2022

Can. J. Physiol. Pharmacol.

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Fingolimod (FTY720) inhibits Ca2+-permeable, Mg2+-sensitive channels called transient receptor potential melastatin 7 (TRPM7), but its effects on Ca2+ paradox (CP)-induced myocardial damage have not been evaluated. We studied the effect of FTY720 on CP-induced myocardial damage, and used other TRPM7 channel inhibitors nordihydroguaiaretic acid (NDGA) and Mg2+ to test if any effect of FTY720 was via TRPM7 inhibition. Langendorff-perfused Wistar rat hearts were treated with FTY720 or NDGA and subjected to a CP protocol consisting of Ca2+ depletion followed by Ca2+ repletion. Hearts of rats pre-treated with MgSO4 were also subjected to CP. Hemodynamic parameters were measured using an intraventricular balloon, and myocardial infarct size was quantified using triphenyltetrazolium chloride stain. TRPM7 proteins in ventricular tissue were detected using immunoblot analysis. FTY720, but not NDGA, decreased CP-induced infarct size. Both FTY720 and NDGA minimized the CP-induced elevation of left ventricular end-diastolic pressure, but only FTY720 ultimately improved ventricular developed pressure. Mg2+ pre-treatment had effect neither on CP-induced infarct size, hemodynamic parameters during CP, nor the level TRPM7 protein expression in ventricular tissue. Overall, FTY720 attenuated CP-induced myocardial damage, with potential therapeutic implications on Ca2+-mediated cardiotoxicity. However, the cardioprotective mechanism of FTY720 seems to be unrelated to TRPM7 channel modulation.

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“…Most frequently used duration for HRV in rat is 5 minutes that is similar to what is being used for analysis in humans. 2,3 Also, one minute, 4,5 3 minutes 6,7 and 10 minutes 8 durations are used often.…”

Section: Introductionmentioning

confidence: 99%

Short term Heart Rate Variability in Rats

Bhagat¹,

Nayak²,

Gupta³

2022

Ind. J. Physiol. Allied Sci.

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Objective: A sustainable animal model is needed to detect Cardiovascular autonomic dysfunctions, which can be assessed by recording electrocardiogram and analysis of heart rate variability (HRV). Therefore, the present study tried to find out the duration for HRV analysis for reliable results.Material and methods: Recording of Electroencephalogram (ECG) is reported in many articles. However, there is no information regarding the duration of ECG to be considered for the HRV analysis. Adult Wistar rat were used for a recording of ECG and HRV analysis. A combination of Ketamine 50 mg/kg and Xylazine 10 mg/kg was used for anesthésia in all the recordings. We analyzed the HRV parameters for all the records with 10 different durations starting from 1 minute to 10 minutes and compared.Results and Discussion: It was observed that, ECG parameters were within normal range while the data from HRV analysis from different duration showed wide discrepancies depending on the duration of ECG recording used for HRV analysis.Conclusion: The minimum duration of eight minutes of recording is likely to produce acceptable data for HRV parameters.

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Beneficial Effects of Magnesium Treatment on Heart Rate Variability and Cardiac Ventricular Function in Diabetic Rats

Abstract: These results suggest that Mg treatment may attenuate diabetes-induced reduction in HRV and improve LV diastolic distensibility, without preventing hyperglycemia and dyslipidemia. Thus, Mg may have a modulatory role in the early stages of diabetic cardiovascular complications.

Cited by 4 publications

References 39 publications

The effects of subchronic intake of magnesium hydro-carbonate-rich mineral water on cardiometabolic markers and electrolytes in rats with streptozotocin-induced diabetes

The effects of subchronic intake of magnesium hydro-carbonate-rich mineral water on cardiometabolic markers and electrolytes in rats with streptozotocin-induced diabetes

Cardioprotective effect of fingolimod against calcium paradox–induced myocardial injury in the isolated rat heart

Short term Heart Rate Variability in Rats

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