Beneficial effects of melatonin on in vitro embryo production from juvenile goat oocytes

Soto-Heras, Sandra; Roura, M.; Catalá, M. G.; Menéndez-Blanco, Irene; Izquierdo, Dolors; Fouladi-Nashta, Ali A.; Paramio, M. T.

doi:10.1071/rd17170

Cited by 38 publications

(24 citation statements)

References 51 publications

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“…Finally, we hypothesized a positive effect of melatonin on embryo development and quality. Contrary to our previous results (Soto‐Heras et al, ) we did not find a higher blastocyst yield with melatonin. Other studies neither found improvement on blastocyst production (Casao et al, ; Rodrigues‐Cunha et al, ; Takada, Martins Junior, Mingoti, Balieiro, & Coelho, ) which denotes a variability in results depending on specific culture conditions.…”

Section: Discussioncontrasting

confidence: 99%

“…There is strong evidence of melatonin positive effect on IVEP when is added to IVM medium in cow and sheep (Sampaio et al, ; Tian et al, ). Previous studies in our laboratory showed an increase of blastocyst production and quality by adding 10 −7 M of melatonin to IVM medium of juvenile goats, which denotes its potential for improving JIVET programs (Soto‐Heras et al, ). In this previous study, we also observed that melatonin decreased oocyte ROS levels.…”

Section: Discussionmentioning

confidence: 63%

“…However, there is a great interest in improving JIVET for animal production as it reduces the generational interval and increases the genetic gain (Morton, ). In a previous study in our laboratory, we showed that 10 −7 M melatonin added to the IVM medium improved blastocyst production and quality in juvenile goats (Soto‐Heras et al, ).…”

Section: Introductionmentioning

confidence: 76%

“…The experimental groups were: control (conventional medium), melatonin (10 −7 M), and melatonin + luzindole (both at 10 −7 M). The melatonin concentration was selected according to our previous results (Soto‐Heras et al, ) and luzindole was added at the same concentration following experimental design of Tian et al ().…”

Section: Methodsmentioning

confidence: 99%

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Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Soto-Heras

Catalá

Roura

et al. 2018

Reprod Domestic Animals

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Contents Melatonin enhances in vitro embryo development in several species by improving the oocyte developmental competence during in vitro maturation (IVM). Melatonin has a wide range of actions, from scavenging reactive oxygen species (ROS) to regulating gene expression, and it can also act by way of melatonin receptors. The aim of this study was to determine the mechanism of action of melatonin during the IVM of juvenile goat oocytes and the role of the membrane receptors. Melatonin receptor 1 was immunolocalized in cumulus cells and oocytes before and after 24 hr of IVM. The effect of melatonin on oocyte developmental competence was tested in three experimental IVM groups: (a) control, (b) 10−7 M melatonin, and (c) 10−7 M melatonin +10−7 M luzindole (an inhibitor of both melatonin receptors). After IVM oocytes were assessed for ROS levels, mitochondrial activity, adenosine 5′‐triphosphate (ATP) concentration and relative gene expression (ACTB, SLC1A1, SOD1, GPx1, BAX, DNMT1, GCLC and GDF9). IVM‐oocytes were in vitro fertilized and cultured under conventional conditions. Blastocyst rate and quality (differential cell count) were assessed at 8 days post‐fertilization. Melatonin decreased ROS levels, increased mitochondrial activity and ATP content and increased blastocyst quality compared to control group (55.8 vs. 30.4 inner cell mass ICM, p < 0.05). There was no effect on the relative gene expression due to treatment with melatonin. In conclusion, we have showed that melatonin improves oocyte developmental competence in juvenile goats by reducing ROS levels and improving mitochondrial activity.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 76%

Section: Methodsmentioning

confidence: 99%

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Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Soto-Heras

Catalá

Roura

et al. 2018

Reprod Domestic Animals

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“…Under in vivo conditions, COCs are provided with all essential minerals, growth factors, proteins, and natural buffering agents that cannot be mimicked totally under in vitro counterparts. However, developmental capabilities of COCs can be improved by supplementation of various hormones, growth factors, serum, cells, follicular fluid, and other substances added to the maturation media (Romero-Arredondo and Seidel, 1996;Choi et al, 2001;Zhou et al, 2016;Soto-Heras et al, 2018). In this context, the blastocyst rate was improved, reaching up to 70% when COCs were matured in vivo, while this rate dropped to 30-35% under IVM conditions (Wrenzycki and Stinshoff, 2013).…”

Section: Introductionmentioning

confidence: 99%

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

Veshkini¹,

Mohammadi‐Sangcheshmeh²,

Ghanem³

et al. 2018

Anim Reprod

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Royal jelly (RJ) was supplemented to goat oocyte in vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Percentage of oocytes that reached MII-stage was increased in RJ-treated groups compared to the control group. Glutathione (GSH) content of mature oocytes was enhanced when RJ was added to IVM medium at any supplementation compared with control. Percentage of cleaved embryos and blastocysts was higher in the RJ-treated groups at a concentration of 5 mg/ml than in the 2.5 mg/ml and control group. Total number of cells per blastocyst was not different in the control and RJtreated group at 5 mg/ml. However, number of apoptotic cells per blastocyst was higher in the control group than in the RJ-treated group at 5 mg/ml. Expression profile of Bax, and p53 was down-regulated while Bcl-2 was upregulated in oocytes treated with RJ at 5 and 10 mg/ml compared with the control group. Addition of RJ at concentrations of 5 mg/ml improved embryo production through increasing maturation rate. RJ seems to improve the IVM microenvironment by reducing expression of genes inducing apoptosis, enhancing GSH content, and reducing incidence of apoptosis in blastocysts.

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Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

Barberino

Macedo

Lins

et al. 2022

Molecular Reproduction Devel

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This study characterized the expression of melatonin receptor type 1 (MT 1 ) protein in sheep ovaries, evaluated melatonin effects on primordial follicle survival and development after in vitro culture of ovarian tissue and verified the possible involvement of the phosphatidylinositol-3-kinase/protein kinase B/forkhead box O3a (PI3K/Akt/FOXO3a) pathway in the melatonin actions. Ovine ovarian fragments were cultured in α-modified minimum essential medium alone (α-MEM + ) or supplemented with 100, 500, or 1000 pg/ml melatonin for 7 days. PI3K inhibition was performed through pretreatment of ovarian fragments with LY294002. Thereafter, immunohistochemistry was performed to evaluate the expression of cleaved caspase-3, Akt, phosphorylated-Akt, and phosphorylated-FOXO3a (p-FOXO3a). The immunohistochemical localization of the MT 1 receptor protein was documented in sheep preantral and antral follicles. After in vitro culture, 100 pg/ml melatonin showed higher follicular survival and activation than α-MEM + and other melatonin concentrations.After PI3K inhibition, there was an increase in cleaved caspase-3-positive follicles, and a decrease in the primordial follicle activation, Akt phosphorylation, and nuclear exclusion of p-FOXO3a. In conclusion, MT 1 receptor protein is present in the sheep ovary.Furthermore, 100 pg/ml melatonin maintains survival and stimulates activation of primordial follicles through the PI3K/Akt/FOXO3a signaling pathway after in vitro culture of sheep ovarian tissue. K E Y W O R D S hormone receptor, immunohistochemistry, in vitro culture, ovarian cortex, preantral follicle 1 | INTRODUCTION In vitro activation of primordial follicles cultured within the ovary itself is an essential tool to study the early follicle development and optimize the use of the female germ cell pool (Telfer & Zelinski, 2013; J. J. Wang et al., 2017). Studies on in vitro activation of primordial follicles, and subsequent growth and maturation have gained a lot of attention as a potential source of mature eggs for in vitro fertilization and embryo transfer, followed by the delivery of healthy born

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Beneficial effects of melatonin on in vitro embryo production from juvenile goat oocytes

Cited by 38 publications

References 51 publications

Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

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