Beneficial effects of prolonged 2-phenylethyl alcohol inhalation on chronic distress-induced anxio-depressive-like phenotype in female mice

Ramadan, Bahrie; Cabeza, Lidia; Cramoisy, Stephanie; Houdayer, Christophe; Andrieu, Patrice; Millot, Jean-Louis; Haffen, E.; Risold, Pierre‐Yves; Peterschmitt, Yvan

doi:10.1016/j.biopha.2022.113100

Cited by 7 publications

(5 citation statements)

References 92 publications

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“…After 5 weeks of NKS3 treatment, animals were sacrificed for evaluation of the neuroinflammation by IBA-1 immunofluorescence. The procedures were similar to those previously described by our team [ 34 , 35 ]. Briefly, after an intraperitoneal injection of pentobarbital (55 mg/kg, Exagon ® , Med’Vet, France), mice were transcardially perfused with 0.9% NaCl, followed by ice cold 4% PFA fixation in 0.1 M phosphate buffer (PB, pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

A novel fatty acid analogue triggers CD36–GPR120 interaction and exerts anti-inflammatory action in endotoxemia

Boutanquoi,

Khan,

Cabeza

et al. 2024

Cell. Mol. Life Sci.

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Inflammation is a mediator of a number of chronic pathologies. We synthesized the diethyl (9Z,12Z)-octadeca-9,12-dien-1-ylphosphonate, called NKS3, which decreased lipopolysaccharide (LPS)-induced mRNA upregulation of proinflammatory cytokines (IL-1β, IL-6 and TNF-α) not only in primary intraperitoneal and lung alveolar macrophages, but also in freshly isolated mice lung slices. The in-silico studies suggested that NKS3, being CD36 agonist, will bind to GPR120. Co-immunoprecipitation and proximity ligation assays demonstrated that NKS3 induced protein–protein interaction of CD36 with GPR120in RAW 264.7 macrophage cell line. Furthermore, NKS3, via GPR120, decreased LPS-induced activation of TAB1/TAK1/JNK pathway and the LPS-induced mRNA expression of inflammatory markers in RAW 264.7 cells. In the acute lung injury model, NKS3 decreased lung fibrosis and inflammatory cytokines (IL-1β, IL-6 and TNF-α) and nitric oxide (NO) production in broncho-alveolar lavage fluid. NKS3 exerted a protective effect on LPS-induced remodeling of kidney and liver, and reduced circulating IL-1β, IL-6 and TNF-α concentrations. In a septic shock model, NKS3 gavage decreased significantly the LPS-induced mortality in mice. In the last, NKS3 decreased neuroinflammation in diet-induced obese mice. Altogether, these results suggest that NKS3 is a novel anti-inflammatory agent that could be used, in the future, for the treatment of inflammation-associated pathologies.

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Section: Methodsmentioning

confidence: 99%

A novel fatty acid analogue triggers CD36–GPR120 interaction and exerts anti-inflammatory action in endotoxemia

Boutanquoi,

Khan,

Cabeza

et al. 2024

Cell. Mol. Life Sci.

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“…Twenty-four hours after the behavioral assessment, animals were euthanized with an intraperitoneal injection of pentobarbital (55 mg/kg, Exagon®, Med’Vet , France) for neuroinflammatory evaluation through ionized calcium-binding adapter molecule 1 (IBA-1) immunofluorescence staining. The procedures were similar to those we previously described 50 , 51 . After, mice were transcardially perfused with 0.9% NaCl, followed by 4% paraformaldehyde fixative (PFA, Roth® , Karlsruhe, Germany) dissolved in 0.1 M phosphate buffer (PB, pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Dietary linoleic acid supplementation protects against obesity-induced microglial reactivity in mice

Jantzen,

Dumontoy,

Ramadan

et al. 2024

Sci Rep

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We investigated whether linoleic acid (LA) supplementation could modulate emotional behavior and microglia-related neuroinflammation. For that, male mice of C57BL/6J genetic background fed either a high-fat diet (HFD) or a standard diet (STD) for 12 weeks, were treated with a vehicle or LA solution for 5 weeks before being evaluated for emotional behavior using a battery of behavioral tests. The animals were subsequently sacrificed and their brains collected and processed for immunofluorescence staining, targeting microglia-specific calcium-binding proteins (IBA-1). Neuroinflammation severity was assessed in multiple hypothalamic, cortical and subcortical brain regions. We show an anxio-depressive-like effect of sustained HFD feeding that was neither alleviated nor worsened with LA supplementation. However, increased IBA-1 expression and microgliosis in the HFD group were largely attenuated by LA supplementation. These observations demonstrate that the anti-neuroinflammatory properties of LA are not restricted to hypothalamic areas but are also evident at the cortical and subcortical levels. This study discloses that neuroinflammation plays a role in the genesis of neuropsychiatric disorders in the context of obesity, and that LA supplementation is a useful dietary strategy to alleviate the impact of obesity-related neuroinflammation.

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“…Animals were food-deprived for 12 hours before being individually placed in an opaque cylindrical open-field apparatus (height: 60 cm; diameter: 47 cm), in the centre of which animals could reach two grain-based pellets located on a white piece of filter paper (diameter: 10 cm). Similarly to previous studies [ 58,59 ], the behaviour of animals was recorded, and their latency the first approach to food was used as index of anxio-depressive-like behaviour. The test lasted a maximum time of 10 min.…”

Section: Novelty Suppressed Feeding (Nsf) Taskmentioning

confidence: 99%

“…Twenty-four hours after the completion of the behavioural assessment, animals were sacrificed for evaluation of the neuroinflammation by IBA-1 immunofluorescence. The procedures were similar to those previously described by our team [ 58,59 ]. Briefly, after an intraperitoneal injection of pentobarbital (55 mg/kg, Exagon®, Med'Vet, France), mice were transcardially perfused with 0.9% NaCl, followed by icecold 4% paraformaldehyde fixative (PFA, Roth®, Karlsruhe, Germany) in 0.1 M phosphate buffer (PB, pH 7.4).…”

Section: Animals Sacrifice and Tissue Samplingmentioning

confidence: 99%

Linoleic acid supplementation reverses microglial response to diet induced-obesity at hypothalamic, cortical and subcortical level in mice

Jantzen,

Dumontoy,

Ramadan

et al. 2023

Preprint

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Obesity is a major risk factor for neuropsychiatric alterations. Fatty regimes lead to systemic and cerebral inflammation, the latest acting through lipotoxicity on hypothalamic structures controlling energy homeostasis. Since literature points to a protective effect of linoleic acid (LA) on mood disorders through the regulation of systemic inflammation, we investigated how five weeks of LA supplementation modulates emotional behaviour and microglia-related neuroinflammation. C57BL/6j mice were fed with either a high-fat (HFD) or a standard diet for 12 weeks, underwent a battery of behavioural tests and were subsequently sacrificed for immunofluorescence staining targeting microglia-specific calcium-binding proteins (IBA-1). Neuroinflammation severity was approximated in multiple hypothalamic, cortical and subcortical regions. Our results show an anxio-depressive-like effect of sustained HFD that neither was alleviated nor worsen with LA supplementation. Increased IBA-1 expression in the HFD group was substantially reversed with LA supplementation. Thus, our results suggest anti-neuroinflammatory properties of LA not restricted to hypothalamic areas, but also evident at the cortical and subcortical level. This study is therefore relevant in the frame of obesity and neuropsychiatric disorders with a neuroinflammatory basis. Further investigation may provide more information to justify dietary strategies aiming at reducing the impact of obesity associated comorbidities.

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Beneficial effects of prolonged 2-phenylethyl alcohol inhalation on chronic distress-induced anxio-depressive-like phenotype in female mice

Cited by 7 publications

References 92 publications

A novel fatty acid analogue triggers CD36–GPR120 interaction and exerts anti-inflammatory action in endotoxemia

A novel fatty acid analogue triggers CD36–GPR120 interaction and exerts anti-inflammatory action in endotoxemia

Dietary linoleic acid supplementation protects against obesity-induced microglial reactivity in mice

Linoleic acid supplementation reverses microglial response to diet induced-obesity at hypothalamic, cortical and subcortical level in mice

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