Beneficial effects of Red Light-Emitting Diode treatment in experimental model of acute lung injury induced by sepsis

Abstract

Sepsis is a severe disease with a high mortality index and it is responsible for the development of acute lung injury (ALI). We evaluated the effects of light-emitting diode (LED) on ALI induced by sepsis. Balb-c mice were injected with lipopolysaccharide or saline and then irradiated or not with red LED on their tracheas and lungs for 150 s, 2 and 6 h after LPS injections. The parameters were investigated 24 h after the LPS injections. Red LED treatment reduced neutrophil influx and the levels of interleukins 1β, 17 A and, tumor necrosis factor-α; in addition to enhanced levels of interferon γ in the bronchoalveolar fluid. Moreover, red LED treatment enhanced the RNAm levels of IL-10 and IFN-γ. It also partially reduced the elevated oxidative burst and enhanced apoptosis, but it did not alter the translocation of nuclear factor κB, the expression of toll-like receptor 4 (TLR4), as well as, oedema or mucus production in their lung tissues. Together, our data has shown the beneficial effects of short treatment with LED on ALI that are caused by gram negative bacterial infections. It is suggested that LED applications are an inexpensive and non-invasive additional treatment for sepsis.

Verification of the Effects of Red Light-emitting Diode Therapy on Acute Lung Injury in a Sepsis Model in Rats

“…Siqueira and coauthors [24] also observed that red LED (660 nm) decreased the lung inflammatory infiltration in an experimental model of asthma. In addition, in earlier studies of our group using phototherapy in lung inflammation model induced by CLP we showed similar effects of phototherapy in the reduction of inflammatory cells of the lung [26]. It may be suggested that the energy offered to the lung tissue was able of inducing an appropriate tissue-response, modulating the inflammatory process.…”

Sunlight Exposure and Phototherapy: Perspectives for Healthy Aging in an Era of COVID-19

“…Furthermore, a recent case report suggested the therapeutic potential of phototherapy with a red light (630 + 660 nm) for the alleviation of respiratory symptoms, pulmonary inflammation, and hypoxia [103]. The beneficial effect of red light (660 nm) was also confirmed in an experimental model of sepsis-associated ALI [104]. Although most peripheral tissues, except the eye and skin, are not normally reached by light, accumulating evidence suggests the direct and indirect impacts of visible light on cell behavior and biological activities through opsin (OPN) receptors [105].…”

A Multifunctional Neutralizing Antibody‐Conjugated Nanoparticle Inhibits and Inactivates SARS‐CoV‐2

“…Although the in vivo photothermal treatment was not performed due to limitations of our biosafety level 3 facilities and the availability of an appropriate light source, the in vitro experiments suggest it may further improve the therapeutic effect of the multifunctional NPs, which is also supported by previous studies. It has been reported that phototherapy can treat lung inflammation [ 84 ] and lung injury [ 85 ] in vivo after red light irradiation on the respiratory tract. In vivo ablation of lung cancer [ 86 ] and other different types of cancers including skin cancer, [ 87 ] breast cancer, [ 88 ] glioblastoma, [ 66 ] mammary cancer, [ 89 ] and colorectal cancer [ 90 ] were achieved by photothermal therapies using red/near‐infrared light excitation.…”