Beneficial effects of sulfonamide-based gallates on osteoblasts in vitro

Abstract

Effective treatments for osteoporosis remain fairly elusive; however, studies have reported that antioxidants may aid in the maintenance of reactive oxygen species at a favorable level, in order to prevent osteoporosis. Gallic acid (GA) and its derivatives are potent antioxidative and anti-inflammatory agents that affect several biochemical and pharmacological pathways; however, GA is slightly cytotoxic and suppresses cell proliferation. The present study modified GA by the introduction of sulfonamide, in order to obtain a novel compound known as JEZ-C, and investigated its effects on osteoblasts by measuring cell proliferation, viability, morphology, alkaline phosphatase (ALP) activity, and the expression of relevant osteoblast markers. Results indicated that JEZ-C may effectively promote osteoblast growth. JEZ-C increased ALP activity, upregulated the expression of osteogenic-related genes, including runt-related transcription factor 2, bone sialoprotein, osteocalcin and alpha-1 type I collagen, thus indicating that JEZ-C enhances bone matrix production and mineralization. The recommended range of JEZ-C concentration is between 6.25×10−3 and 6.25×10−1 µg/ml, within which cell growth was promoted compared with the control. Specifically, treatment with 6.25×10−2 µg/ml JEZ-C is ideal. These findings may represent a novel approach to cell-based therapy for the treatment of osteoporosis.

Study on the Anticoagulant or Procoagulant Activities of Type II Phenolic Acid Derivatives

“…and has obtained some polysaccharide, flavonoid, and phenolic acid compounds [ 10 , 11 , 23 , 24 , 25 , 26 , 27 , 28 ]. Additionally, the obtained flavonoids and phenolic acids were used as parent compounds to synthesize a series of derivatives, and their haemostatic and anticoagulant activities were evaluated by several bioassays [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. According to our previous research results, phenolic acid compounds separated from Blumea riparia DC.…”

In vitro and in vivo effect of novel GA‐CSNPs loaded col‐fibrin nanocomposite scaffold on diabetic wound healing

“…45,46 In addition to the antiinflammatory effect, Gallic acid also enhances collagen I and extracellular matrix synthesis. 47 It was proven that Gallic acid effectively promoted wound healing with the activation of c-Jun N-terminal kinases (JNK), focal adhesion kinases (FAK), and extracellular signal-regulated kinases (Erk), increased fibroblast and keratinocyte proliferation as well as migration and decreased apoptosis in the keratinocyte and fibroblast cells. 48 Treatment with Gallic acid also helps to accelerate wound contraction, increases hydroxyproline content in the granulation tissues, helps to provide high tensile strength, increases collagen synthesis and deposition, and increases the vascularization process with decreased IL-6, NO, and TNF-α levels.…”

Gallic Acid Derivative Stimulate Osteogenic Differentiation by Regulating Autophagy via JNK/mTOR Signaling Pathways in vitro

“…The stock solution was stored at 4˚C for one week and diluted with culture medium immediately before the experiment. Our previous studies have show that gallic acid derivative ranged from 6.25x10-3 to 6.25x10-1 μg/ml significantly promoted cell growth [18,19]. Therefore，cells were treated with the culture medium containing various concentrations of 4-O-methylgallic acid(0 μg/ml as control, 6.25x10-3, 6.25x10-2 and 6.25x10-1 μg/ml) for the following researches.…”