Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.

Savić﻿﻿﻿, Jelena; Nikolić, Radomirka; Banjac, Nevena; Zdravković-Korać, Snežana; Stupar, Sofija; Cingel, Aleksandar; Ćosić﻿﻿﻿, Tatjana; Raspor, Martin; Smigocki, Ann C.; Ninković﻿﻿﻿, Slavica

doi:10.1016/j.jplph.2019.153055

Cited by 9 publications

(5 citation statements)

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“…Due to the increase in salt of agricultural soils, forage legumes experience yield losses and low nutritional status (Savić et al, 2019). Although salt stress impacts a plant's growth at every stage, most plant species are known to be particularly vulnerable at the seed germination and seedling growth stages (Bybordi, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…L. corniculatus a perennial legume forage is regarded as one of the most important forage plants in terms of agriculture due to its many benefits, including its ability to grow in low fertile, acidic, and its anti-bloating properties due to its tannin content, and it is also frequently used to stop roadside erosion (Wang et al, 2021). In temperate places of the world, L. corniculatus may eventually take the place of white clover and alfalfa (Savić et al, 2019). Despite all these important features, most L. corniculatus are sensitive to salinity (Bao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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NaCl Kaynaklı Tuz Stresinin Lotus corniculatus L. cv. 'Leo'nun Çimlenmesi ve İlk Fide Büyümesi Üzerindeki Etkisi

BEYAZ,

KAZANKAYA

2024

Tekirdağ Ziraat Fakültesi Dergisi

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Lotus corniculatus L. is one of the agronomically and economically important perennial legume forage species with moderately salt-tolerant. It is well known that even the cultivars of the same species in plants have different responses in salinity. However, studies on the salt response of L. corniculatus and its cultivars, which are more advantageous than other forage crops such as white clover (Trifolium repens) and alfalfa (Medicago sativa L.), in the use of marginal agricultural lands affected by abiotic stress factors such as salinity, are limited. Under salt stress, the most crucial phases of the plant life cycle that are directly related to the survival of the plant are seed germination, growth, and vigour. Therefore, this study was carried out to determine the germination and growth responses of L. corniculatus cultivar 'Leo', which is known to have higher tannin content than other cultivars, under NaCl-derived salt stress in vitro. For this purpose, L. corniculatus seeds were cultured in MS (Murashige and Skoog/Gamborg) medium containing 0, 40, and 80 mM NaCl for 14 days. Seed germination percentage, mean germination time, germination rate index, shoot-root length, root to shoot length ratio, shoot-root fresh dry weight, shoot-root dry matter, the ratio of root to shoot dry matter, shoot-root water content and seedling vigour index parameters were measured. According to the results of the research, the germination percentage did not change in the applied NaCl treatments, but the germination rate decreased. However, shoot length decreased and root length increased. Although there was no statistically significant change in shoot and root fresh-dry weight, both decreased in 80 mM NaCl treatment. The shoot and root dry matter increased and the water content decreased. Also, the seedling viability index decreased. In 40 mM NaCl treatment, on the other hand, there was an increase in shoot fresh-dry weight, dry matter ratio and seedling viability index with the positive effect of low dose. Within the scope of this study, comprehensive information was presented for L. corniculatus (cultivar 'Leo'), an important forage plant, in terms of germination and seedling growth under salt stress.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NaCl Kaynaklı Tuz Stresinin Lotus corniculatus L. cv. 'Leo'nun Çimlenmesi ve İlk Fide Büyümesi Üzerindeki Etkisi

BEYAZ,

KAZANKAYA

2024

Tekirdağ Ziraat Fakültesi Dergisi

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Section: Methodsmentioning

confidence: 99%

“…The salinity injury index (SII) ( Diaz-Vivancos et al., 2013 ; Savić et al., 2019 ) was evaluated as a general measure of salt-induced damage to the stressed plants, as described in Table 1 . Modifications to the previous descriptions of SII ( Diaz-Vivancos et al., 2013 ; Savić et al., 2019 ) were introduced to match the specific effects of salinity damage to the in vitro grown potato plants. The SII values for a particular genotype at a given experimental treatment were calculated as the mean of the individual scoring of at least 30 plantlets, performed independently by two researchers with almost identical results.…”

Section: Methodsmentioning

confidence: 99%

Cytokinin deficiency confers enhanced tolerance to mild, but decreased tolerance to severe salinity stress in in vitro grown potato

Raspor,

Mrvaljević,

Savić

et al. 2024

Front. Plant Sci.

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Cytokinin (CK) is a plant hormone that plays crucial roles in regulating plant growth and development. CK-deficient plants are widely used as model systems for investigating the numerous physiological roles of CK. Since it was previously shown that transgenic or mutant CK-deficient Arabidopsis and Centaurium plants show superior tolerance to salinity, we examined the tolerance of three CK-deficient potato lines overexpressing the Arabidopsis thaliana CYTOKININ OXIDASE/DEHYDROGENASE2 (AtCKX2) gene to 50 mM, 100 mM, 150 mM, and 200 mM NaCl applied in vitro. Quantification of visible salinity injury, rooting and acclimatization efficiency, shoot growth, water saturation deficit, and chlorophyll content confirmed that the CK-deficient potato plants were more tolerant to low (50 mM) and moderate (100 mM) NaCl concentrations, but exhibited increased sensitivity to severe salinity stress (150 and 200 mM NaCl) compared to non-transformed control plants. These findings were corroborated by the data distribution patterns according to principal component analysis. Quantification of the activity of superoxide dismutases, peroxidases, and catalases revealed an impaired ability of AtCKX2-transgenic lines to upregulate the activity of antioxidant enzymes in response to salinity, which might contribute to the enhanced sensitivity of these potato lines to severe salt stress. Our results add complexity to the existing knowledge on the regulation of salinity tolerance by CK, as we show for the first time that CK-deficient plants can exhibit reduced rather than increased tolerance to severe salt stress.

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“…The amplification was carried out in a thermal cycler (Applied Biosystems, Gene Amp PCR Systems 9700, Waltham, MA, USA), using specific primers according to reports in each case. rolA: 5 ′ -CGTTGTCGGAATGGCCCAGACC-3 ′ and 3 ′ -CGTAGGTCTGAATATTCCGGTCC-5 ′ to amplify a 248 bp fragment, rolB: 5 ′ -ACTATAGCAAACCCCTCCTGC-3 ′ and 3 ′ -TTCAGGTTTACTGCAGCAGGC-5 ′ , to amplify a 652 bp fragment [33], rolC: 5 ′ -TGTGACAAGCAGCGATGAGC-3 ′ and 3 ′ -GATTGC AAACTTGCACTCGC-5 ′ to amplify a 487 bp fragment, rolD: 5 ′ -CCTTACGAATTCTCTTAG CGGCACC-3 ′ and 3 ′ -GAGGTACACTGGACTGAATCTGCAC-5 ′ to amplify a 477 bp fragment [34] and aux1: 5 ′ -CCAAGCTTGTCAGAAAACTTCAGGG-3 ′ and 3 ′ -CCGGATCCAAT ACCCAGCGCTTT-5 ′ to amplify a 815 bp fragment [35]. DNA electrophoresis was performed in a 1.5% agarose gel at 95V for 60 min.…”

Section: Dna Extraction and Pcr Analysismentioning

confidence: 99%

Chemical Profile of Cell Cultures of Kalanchoë gastonis-bonnieri Transformed by Agrobacterium rhizogenes

Barrera Núñez,

Bueno,

Molina-Montiel

et al. 2024

Agronomy

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Kalanchoë gastonis-bonnieri Raym.-Hamet & Perrier is a plant used for medicinal purposes in the treatment of several ailments. The aim of this study was to analyze the chemical profile of extracts from K. gastonis-bonnieri embryogenic calli, generated from genetically transformed roots by Agrobacterium rhizogenes. Putative transformants were verified by PCR. Hydroalcoholic extracts were obtained and the chemical profile was analyzed by LC-ESI-MS/MS. Root formation was obtained from 80% of infected seedlings. Fifteen root lines were isolated, and two lines showed prominent longitudinal growth and profuse branching in the B5 semi-solid medium. In all lines, the formation of nodules and later embryogenic callus was observed. Putative transgenic root lines were cultivated in free-plant growth regulators B5 medium. In the two selected lines, the PCR amplification of rolA, rolB, rolC, rolD, and aux1 genes was detected. The extract of embryogenic calli showed 60 chemical compounds tentatively identified, such as ferulic acid, quinic acid, neobaisoflavone, and malic acid, among others, and the chemical profile was different in comparison to wild-type extracts. This is the first study reporting the analysis of the chemical profile of hairy root extracts derived from Kalanchoë gastonis-bonnieri. This work displays the great potential for obtaining chemical compounds of pharmacological importance from hairy roots and facilitates the identification of new useful drugs against human chronic-degenerative diseases.

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Beneficial implications of sugar beet proteinase inhibitor BvSTI on plant architecture and salt stress tolerance in Lotus corniculatus L.

Cited by 9 publications

References 78 publications

NaCl Kaynaklı Tuz Stresinin Lotus corniculatus L. cv. 'Leo'nun Çimlenmesi ve İlk Fide Büyümesi Üzerindeki Etkisi

NaCl Kaynaklı Tuz Stresinin Lotus corniculatus L. cv. 'Leo'nun Çimlenmesi ve İlk Fide Büyümesi Üzerindeki Etkisi

Cytokinin deficiency confers enhanced tolerance to mild, but decreased tolerance to severe salinity stress in in vitro grown potato

Chemical Profile of Cell Cultures of Kalanchoë gastonis-bonnieri Transformed by Agrobacterium rhizogenes

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