Beneficial influence of topical extra virgin olive oil application on an experimental model of penile fracture in rats

Abstract

Penile fracture (PF) is known as a traumatic rupture of the tunica albuginea of corpus cavernosum. In this study, we aimed to investigate the healing influence of topical extra virgin olive oil (EVOO) on PF through evaluating levels of some oxidative stress biomarkers for the first time. Histopathological evaluation was also realized. A total of 18 male Sprague-Dawley albino rats were divided into three groups of six rats each as control group, in PF (alone) group, and PF + EVOO group. Experimental PF was formed via incising from the proximal dorsal side of the penis in the rats of all groups except control. While in PF (alone) group, fracture was formed and the incision was primarily closed, in PF + EVOO group in addition to foregoing processes, EVOO was also administrated topically twice a day for 3 weeks. At the end of the experiment, all rats were killed and penectomy was carried out. While malondialdehyde, myeloperoxidase, lipid hyroperoxide, and total oxidant status significantly (p < 0.05) increased, reduced glutathione and total free sulfhydryl groups markedly (p < 0.05) decreased in PF (alone) group when compared with PF + EVOO group. Levels of these parameters were reversed to nearly normal values by topical EVOO application. Protection by EVOO is further substantiated via the improved histological findings in PF + EVOO group as against degenerative changes in the rats of PF (alone) group. Our data revealed that EVOO has protective effect in penile cavernosal tissue through probably its antioxidant, free radical defusing, anti-inflammatory, and antimicrobial effects.

The effects of topical aloe vera on an experimentally designed penile fracture model in rats

“…[27] Bleeding and hyperemia in tissues due to trauma and surgical interventions are expected outcomes. In our study, hyperemia and bleeding were observed in all groups, similar to the study by Akgül et al [23] In another study conducted by Akgül et al, when cyanoacrylic glue (CG) was applied to PF animals, a decrease in hyperemia was observed, attributed to the hemostatic property of CG. [6] Therefore, we observed hyperemia and bleeding in all rat tissues.…”

“…Akgül et al reported that fibrosis following PF repair may be related to the suture material. [23] However, in other studies by Akgül et underwent primary repair after PF. [6,7] Similarly, in our study, it was observed that fibrosis did not increase in rats that underwent primary repair.…”

“…Contrarily, Akgül et al observed that AV application reduces fibrosis. [ 23 ] After wound formation, inflammation mediates the balance between wound healing and fibrosis. [ 29 ] The use of anti-inflammatory agents aids in wound healing but also negatively affects cells that prevent fibrosis development.…”

“…[22] Various studies have evaluated wound healing after PF in the English literature. [6,7,21,[23][24][25] Scar tissue development has been reported in 25% of patients in the area after PF repair. [26] Due to the positive effects on wound healing, we examined the topical effect of a solution obtained from the freeze-dried powder form of AV.…”