Beneficial influence of Vero cells on in vitro maturation and fertilization of bovine oocytes

Grocholová, R.; Petr, Jaroslav; Marek, Jindřich; Teplá, O

doi:10.1016/0093-691x(95)00169-9

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Because our data in the present study showed that Vero cells, except on the first day of cultivation, were unable to improve the rate of development of fresh, nonvitrified embryos, even in the last day of culture the rate of hatched embryos in Vero cells was lower than that of the cellfree medium. This result disagreed with authors who claimed the beneficial effect of Vero cells for improve-ment of the embryos in bovine (31), mouse (32), monkey (33), and human (34), but confirmed the finding of other investigators who failed to demonstrate a clear beneficial effect of Vero cells on the development of mouse embryo (28).…”

Section: Discussioncontrasting

confidence: 90%

Untitled

Valojerdi

Movahedin

Hosseini

2002

Journal of Assisted Reproduction and Genetics

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Section: Discussioncontrasting

confidence: 90%

Untitled

Valojerdi

Movahedin

Hosseini

2002

Journal of Assisted Reproduction and Genetics

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“…Briefly, the COC grades 1, 2, and 3 from each animal were matured in vitro for 22-24 h in 50-l microdrops of M199 supplemented with 10% FCS, 10 g/ml FSH, 10 g/ml LH, and 1 g/ml estradiol 17␤ (Sigma) over a layer of Vero cells (Rhone-Mérieux, Lyon, France) to improve maturation of denuded oocytes [24]. One microdrop was assigned to each cow and all oocytes collected from that animal in one OPU session were matured as a group.…”

Section: In Vitro Maturationmentioning

confidence: 99%

In Vitro Embryo Production Efficiency in Cattle and Its Association with Oocyte Adenosine Triphosphate Content, Quantity of Mitochondrial DNA, and Mitochondrial DNA Haplogroup

Tamassia

Nuttinck

May‐Panloup

et al. 2004

Biology of Reproduction

110

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Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided follicular aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means +/- SEM) both at the germinal vesicle and metaphase II stages (2.8 +/- 0.06 pmol vs. 2.6 +/- 0.07 pmol and 2.9 +/- 0.1 pmol vs. 2.3 +/- 0.06 pmol, respectively). The proportion of development to blastocyst was significantly different between haplogroups (22.3 +/- 2.1 % vs. 36.7 +/- 2.9 %). The number of mtDNA molecules per oocyte was highly variable (377 327 +/- 14 104, ranging from 2.0 x 10(3) to 1.2 x 10(6)) but not significantly different between the two haplogroups; significant differences were observed between animals without any apparent relationship to blastocyst production. These data suggest that mitochondria and mtDNA haplogroup affect the developmental capacity of bovine oocytes in vitro.

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“…de 25mM de Hepes, 100UI/m de penicilina, 50µg/m de estreptomicina e 1,0mg/m de PVA. Em seguida, os ovários foram submetidos a um processo de dissociação, utilizando o método mecânico para isolamentode FPs, desenvolvido por FIGUEIREDO et al (1993) e adaptado por CARÁMBULA et al(1998).Células ovarianas, obtidas por esse processo, foram divididas em dois grupos, com (Grupo FSH) e sem (Grupo controle) FSH, cultivadas em meio TCM-199 modificado acrescido de 10% de soro de novilho castrado (SNC) em gotas de 50µ(GROCHOLOVÁ et al, 1995), cobertas com óleo silicone e incubadas em estufa contendo uma atmosfera de 5% de CO 2 em ar, umidade saturada e temperatura de 39°C. O FSH foi adicionado na concentração de 1,0µg/m de meio.…”

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“…O FSH foi adicionado na concentração de 1,0µg/m de meio. O número de células foi ajustado a uma concentração final de 2x10 4 células/ml de meio de cultivo, contadas através de uma câmara de Neubauer(GROCHOLOVÁ et al, 1995). As placas, contendo uma monocamada confluente de células, foram submetidas à coloração vital pelo método descrito porKRAUSE et al (1984), utilizando Azul de Tripan como corante vital.…”

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Desenvolvimento De Folículos Pré-Antrais Bovinos in Vitro Em Monocamada De Células Ovarianas

Costa¹,

Gonçalves

Figueiredo

et al. 2001

Cienc. Rural

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O presente trabalho teve como objetivos determinar a influência de células ovarianas no desenvolvimento in vitro de folículos pré-antrais, avaliar a viabilidade das células ovarianas em monocamada e a influência do soro na manutenção de folículos pré-antrais in vitro. Folículos pré-antrais (FPs) e células ovarianas foram isolados de ovários de fetos bovinos, com idade entre 6 e 8 meses de gestação, oriundos de matadouro. Células ovarianas em monocamada foram cultivadas em meio TCM-199, e a viabilidade celular, após o cultivo na presença ou ausência de FSH, foi determinada com o corante vital azul de tripan. FPs foram distribuidos em quatro tratamentos e cultivados em TCM-199 modificado, contendo soro de novilho castrado (SNC), SNC em monocamada de células ovarianas (MCO), MCO com FSH ou meio definido com álcool polivinílico (PVA) como macromolécula. A viabilidade celular não foi afetada em conseqüência da presença ou ausência de FSH. No entanto, houve um incremento significativo no tamanho dos FPs cultivados na presença de SNC, MCO e FSH (P<0,05), o que não foi observado nos demais tratamentos. A adição de FSH ao meio de cultivo não influencia a viabilidade de células ovarianas ao longo de 20 dias. Folículos pré-antrais, com área superficial entre 4000 e 8000mim², mas não folículos com área superficial superiores a 8000mim², crescem in vitro na presença de células ovarianas, independentemente da presença de FSH.

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Beneficial influence of Vero cells on in vitro maturation and fertilization of bovine oocytes

Cited by 8 publications

References 22 publications

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In Vitro Embryo Production Efficiency in Cattle and Its Association with Oocyte Adenosine Triphosphate Content, Quantity of Mitochondrial DNA, and Mitochondrial DNA Haplogroup

Desenvolvimento De Folículos Pré-Antrais Bovinos in Vitro Em Monocamada De Células Ovarianas

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