Beneficial or detrimental: Recruiting more types of benign cases for cancer diagnosis based on salivary glycopatterns

Shu, Jiwu; Ren, Xiaolin; Cheng, Hongwei; Wang, Shiyi; Yue, Lixin; Li, Xia; Yin, Mengqi; Chen, Xiangqin; Zhang, Tiantian; Hui, Ziye; Bao, Xiaojuan; Song, Wanghua; Yu, Hanjie; Dang, Liuyi; Zhang, Chen; Wang, Daming; Zhao, Qian; Li, Zheng

doi:10.1016/j.ijbiomac.2023.126354

Cited by 2 publications

(1 citation statement)

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“…The extracted NAs were labeled with Cy3 fluorescent dye (GE Healthcare) and purified using Sephadex G-25 columns following the manufacturer’s instructions. The detailed design and fabrication of the lectin microarray have been described in previous reports [ 17 , 24 ]. Simply put, 4 μg of Cy5-labeled NA was diluted in 0.5 mL of incubation buffer and incubated with the homemade lectin microarrays for 3 h. Each slide was washed by 1 × PBST (Phosphate-Buffered Saline with Tween 20) and 1 × PBS (Phosphate-Buffered Saline) alternately.…”

Section: Methodsmentioning

confidence: 99%

N-Glycan Profiles of Neuraminidase from Avian Influenza Viruses

Chen,

Ma,

Liu

et al. 2024

Viruses

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The cleavage of sialic acids by neuraminidase (NA) facilitates the spread of influenza A virus (IV) descendants. Understanding the enzymatic activity of NA aids research into the transmission of IVs. An effective method for purifying NA was developed using p-aminophenyloxamic acid-modified functionalized hydroxylated magnetic particles (AAMPs), and from 0.299 to 0.401 mg of NA from eight IV strains was isolated by 1 mg AAMP. A combination of lectin microarrays and MALDI-TOF/TOF-MS was employed to investigate the N-glycans of isolated NAs. We found that more than 20 N-glycans were identified, and 16 glycan peaks were identical in the strains derived from chicken embryo cultivation. Multi-antennae, bisected, or core-fucosylated N-glycans are common in all the NAs. The terminal residues of N-glycans are predominantly composed of galactose and N-acetylglucosamine residues. Meanwhile, sialic acid residue was uncommon in these N-glycans. Further computational docking analysis predicted the interaction mechanism between NA and p-aminophenyloxamic acid.

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Section: Methodsmentioning

confidence: 99%