Benefits of Hybrid SPECT/CT for 111In-Oxine- and Tc-99m-Hexamethylpropylene Amine Oxime-Labeled Leukocyte Imaging

Djekidel, Mehdi; Brown, Richard K.; Piert, Morand

doi:10.1097/rlu.0b013e31821738a0

Cited by 28 publications

(21 citation statements)

References 27 publications

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“…Several research groups routinely use 111 In-oxine, which is a SPECT tracer, for immune cell labeling, to reveal non-identified sites of inflammation in patients or to study cell trafficking in rodents (26,27). However, we detected with 0.7 MBq of 111 In-oxine-labeled murine OVA-Th1 cells an induction of double-strand breaks (unpublished results), which even was more severe than with 64 Cu.…”

Section: Intracellular Localization Of 64 Cu and Dna Damagementioning

confidence: 99%

In Vivo Tracking of Th1 Cells by PET Reveals Quantitative and Temporal Distribution and Specific Homing in Lymphatic Tissue

et al. 2014

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Although T cells can be labeled for noninvasive in vivo imaging, little is known about the impact of such labeling on T-cell function, and most imaging methods do not provide holistic information about trafficking kinetics, homing sites, or quantification. Methods: We developed protocols that minimize the inhibitory effects of 64 Cupyruvaldehyde-bis(N4-methylthiosemicarbazone) ( 64 Cu-PTSM) labeling on T-cell function and permit the homing patterns of T cells to be followed by PET. Thus, we labeled ovalbumin (OVA) T-cell receptor transgenic interferon (IFN)-g-producing CD4 1 T (Th1) cells with 0.7-2.2 MBq of 64 Cu-PTSM and analyzed cell viability, IFN-g production, proliferation, apoptosis, and DNA double-strand breaks and identified intracellular 64 Cu accumulation sites by energy dispersive x-ray analysis. To elucidate the fate of Th1 cell homing by PET, 10 7 64 Cu-OVA-Th1 cells were injected intraperitoneally or intravenously into healthy mice. To test the functional capacities of 64 Cu-OVA-Th1 cells during experimental OVA-induced airway hyperreactivity, we injected 10 7 64 Cu-OVA-Th1 cells intraperitoneally into OVA-immunized or nonimmunized healthy mice, which were challenged with OVA peptide or phosphate-buffered saline or remained untreated. In vivo PET investigations were followed by biodistribution, autoradiography, and fluorescence-activated cell sorting analysis. Results: PET revealed unexpected homing patterns depending on the mode of T-cell administration. Within 20 min after intraperitoneal administration, 64 Cu-OVA-Th1 cells homed to the perithymic lymph nodes (LNs) of naive mice. Interestingly, intravenously administered 64 Cu-OVA-Th1 cells homed predominantly into the lung and spleen but not into the perithymic LNs. The accumulation of 64 Cu-OVA-Th1 cells in the pulmonary LNs (6.8 6 1.1 percentage injected dose per cubic centimeter [%ID/cm 3 ]) 24 h after injection was highest in the OVA-immunized and OVA-challenged OVA airway hyperreactivity-diseased littermates 24 h after intraperitoneal administration and lowest in the untreated littermates (3.7 6 0.4 %ID/cm 3 ). As expected, 64 Cu-OVA-Th1 cells also accumulated significantly in the pulmonary LNs of nonimmunized OVA-challenged animals (6.1 6 0.5 %ID/cm 3 ) when compared with phosphate-buffered saline-challenged animals (4.6 6 0.5 %ID/cm 3 ). Conclusion: Our protocol permits the detection of Th1 cells in single LNs and enables temporal in vivo monitoring of T-cell homing over 48 h. This work enables future applications for 64 Cu-PTSM-labeled T cells in clinical trials and novel therapy concepts focusing on T-cell-based immunotherapies of autoimmune diseases or cancer.

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Section: Intracellular Localization Of 64 Cu and Dna Damagementioning

confidence: 99%

In Vivo Tracking of Th1 Cells by PET Reveals Quantitative and Temporal Distribution and Specific Homing in Lymphatic Tissue

et al. 2014

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“…The proposed technique is analogous to prior published work on 2-phase or 3-phase 18 F-FDG PET for chronic osteomyelitis (6). 18 F-FDG PET for imaging of osteomyelitis has been found to have excellent sensitivity and specificity for bone infection, with possibly even higher accuracy than the cur- rent gold standard radionuclide technique of 99m Tc-hexamethylpropyleneamine oxime-or 111 In-labeled white blood cell scintigraphy (7,8). Whether the addition of an early phase could augment the 18 F-FDG PET scan and further improve its diagnostic capability is an intriguing question.…”

Section: Replymentioning

confidence: 75%

Reply: Regarding Dynamic Bone Imaging with ^99mTc-Labeled Diphosphonates and ¹⁸F-NaF: Mechanisms and Applications

Wong

Piert

2013

J Nucl Med

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“…Die Anzahl wissenschaftlicher Arbeiten zum Stellenwert der SPECT/CT für die Infektbildgebung ist gegenwärtig noch begrenzt [1,4,5,6,7,8,9]. Die vorliegende Arbeit gibt aus diesem Grund neben Erkenntnissen der publizierten Literatur auch persönliche Erfahrungen und Einschätzungen der Autoren wieder.…”

Section: Diagnosestellungunclassified

“…Im Vergleich zur planaren Szintigraphie erlaubt die SPECT/CT somit eine spezifischere und detailliertere Beurteilung. Die SPECT/CT erbringt bei 36-53% der IS einen wesentlichen Beitrag zur endgülti-gen Diagnose [5,6] und minimiert die Rate an unklaren oder zweifelhaften Untersuchungsresultaten nach planarer und SPECT-Bildgebung [4,10]. …”

Section: Zusammenfassung · Abstractunclassified

SPECT/CT-Infektdiagnostik am Skelett

2012

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Benefits of Hybrid SPECT/CT for 111In-Oxine- and Tc-99m-Hexamethylpropylene Amine Oxime-Labeled Leukocyte Imaging

Abstract: Hybrid SPECT/CT leukocyte imaging has incremental value over planar imaging with SPECT because of improved diagnostic accuracy of lesion identification and reader confidence.

Cited by 28 publications

References 27 publications

In Vivo Tracking of Th1 Cells by PET Reveals Quantitative and Temporal Distribution and Specific Homing in Lymphatic Tissue

In Vivo Tracking of Th1 Cells by PET Reveals Quantitative and Temporal Distribution and Specific Homing in Lymphatic Tissue

Reply: Regarding Dynamic Bone Imaging with ^99mTc-Labeled Diphosphonates and ¹⁸F-NaF: Mechanisms and Applications

SPECT/CT-Infektdiagnostik am Skelett

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Benefits of Hybrid SPECT/CT for 111In-Oxine- and Tc-99m-Hexamethylpropylene Amine Oxime-Labeled Leukocyte Imaging

Abstract: Hybrid SPECT/CT leukocyte imaging has incremental value over planar imaging with SPECT because of improved diagnostic accuracy of lesion identification and reader confidence.

Cited by 28 publications

References 27 publications

In Vivo Tracking of Th1 Cells by PET Reveals Quantitative and Temporal Distribution and Specific Homing in Lymphatic Tissue

In Vivo Tracking of Th1 Cells by PET Reveals Quantitative and Temporal Distribution and Specific Homing in Lymphatic Tissue

Reply: Regarding Dynamic Bone Imaging with 99mTc-Labeled Diphosphonates and 18F-NaF: Mechanisms and Applications

SPECT/CT-Infektdiagnostik am Skelett

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Reply: Regarding Dynamic Bone Imaging with ^99mTc-Labeled Diphosphonates and ¹⁸F-NaF: Mechanisms and Applications