Virtual reality (VR) has emerged as a promising tool for enhancing practical skills of students in science and engineering education. However, the effectiveness of VR in this context remains unclear due to inconsistent findings across studies. This meta-analysis aimed to synthesize the existing literature and investigate the overall impact of VR on practical skills among science and engineering students. A comprehensive literature search was conducted, yielding 37 empirical studies published between 2000 and 2022 that met the inclusion criteria. The analysis included 72 effect sizes, and the random-effects model was employed to account for heterogeneity among studies. The results revealed a significant moderate positive effect of VR on practical skills (g = 0.477). Moderator analyses indicated that the disciplinary category significantly influenced the effect size, with medical students demonstrating the largest improvement in practical skills. Additionally, using the practice approach combining with traditional methods yielded the highest effect size among the instructional approaches. The study also considered potential reasons behind the observed results and acknowledged certain constraints. Additionally, it proposed avenues for further inquiry to advance the understanding of the subject matter.