Benomyl resistance of Colletotrichum acutatum is caused by enhanced expression of β-tubulin 1 gene regulated by putative leucine zipper protein CaBEN1

Nakaune, Ryoji; Nakano, Mikio

doi:10.1016/j.fgb.2007.03.007

Cited by 48 publications

(31 citation statements)

References 54 publications

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“…1), higher concentrations of fungicides did not further reduce colony (μg/ml) MIC (μg/ml) EC diameter. Recently, Nakaune and Nakano (2007) reported that benomyl resistance was due to enhanced expression of the C. acutatum tubulin (CaTUB1) gene in response to benomyl, a member of the BENs. They demonstrated that CaBEN1, a putative leucine zipper protein that regulates the expression of CaTUB1, was necessary for resistance to BENs.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of Colletotrichum spp. Isolated from Grapes in Korea to Carbendazim and the Mixture of Carbendazim Plus Diethofencarb

Hwang¹,

Kim²,

Kim³

et al. 2010

The Plant Pathology Journal

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Section: Discussionmentioning

confidence: 99%

Sensitivity of Colletotrichum spp. Isolated from Grapes in Korea to Carbendazim and the Mixture of Carbendazim Plus Diethofencarb

Hwang¹,

Kim²,

Kim³

et al. 2010

The Plant Pathology Journal

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“…Para el control en campo de Colletotrichum spp. así como otros hongos fitopatógenos, se suele emplear fungicidas de tipo benzimidazol como el Benomil; sin embargo el rápido desarrollo de cepas resistentes a este compuesto ha limitado su amplio uso (Nakaune & Nakano 2007). Por lo anterior, se hace necesario la implementación de otro tipo de sustancias para combatir las enfermedades en los cultivos.…”

Section: Introductionunclassified

Caracterización química y evaluación de la actividad antifúngica del aceite esencial foliar de Lippia alba contra Colletotrichum gloeosporioides

2017

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Información sobre los autores:APC, LCA: realizaron el diseño experimental; DVR realizó la extracción del aceite esencial y el análisis cromatográfico; DVR: realizó los ensayos in vitro, realizó el análisis estadístico y redactó el manuscrito; ALP; LCA supervisaron todas las actividades; APC, LCA, DVR: revisaron y aprobaron el manuscrito.Los autores no incurren en conflictos de intereses. ResumenEl objetivo de este estudio fue caracterizar y evaluar in vitro la eficiencia del aceite esencial foliar de Lippia alba contra Colletotrichum gloeosporioides. Se extrajo el aceite esencial a partir de las hojas mediante el método de hidrodestilación asistida por microondas (MWHD) y se identificaron sus metabolitos mediante una cromatografía de gases acoplada a espectrometría de masas (GC/MS). La evaluación in vitro se realizó empleando 4 concentraciones del aceite esencial (500, 1000, 3000 y 10000 ppm) diluidas en acetona; se utilizó un control positivo con Benomil 1 g/L, un control negativo con acetona y un testigo absoluto; la eficiencia del aceite se midió mediante el porcentaje de índice antifúngico (%I.A). El componente mayoritario en el aceite esencial fue el citral (34.62 a 40.03%) y que el mayor %I.A se encontró a la concentración de 10000 ppm (97.8%), muy similar a la del Benomil (100%). Lo anterior demostraría la eficacia del aceite esencial de L. alba para controlar a C. gloeosporioides, y su posible uso como fungicida biológico.Palabras clave: Aceites esenciales; fitopatógenos; fungicidas; antracnosis; Lippia alba; Colletotrichum gloeosporioides. AbstractThe aim of this study was to characterize and evaluate the in vitro efficiency of leaf essential oil of Lippia alba against Colletotrichum gloeosporioides. Essential oil from the leaves was extracted by the method of assisted microwave hydrodistillation (MWHD) and metabolites were identified by gas chromatography coupled to mass spectrometry (GC/ MS). The in vitro evaluation was performed using 4 concentrations of essential oil (500, 1000, 3000 and 10000 ppm) diluted in acetone; positive control with Benomyl 1 g /L, a negative control with acetone and absolute control was used; oil efficiency was measured by the percentage of antifungal index (% IA). It was found that the major component was in the essential oil citral (34.62 to 40.03%) and the highest %I.A was found to 10000 ppm concentration (97.8%) very similar to Benomyl (100%). This would demonstrate the efficacy of the L. alba essential oil to control C. gloeosporioides, and its possible use as a biological fungicide.

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“…For example, many fungal pathogens have developed resistance to BZs via amino acid changes in the β-tubulin protein [35], [39], while the inherent benomyl-resistance of Colletotrichum acutatum results from enhanced expression of the β-tubulin 1 gene [40]. However, although mutations in the β-tubulin genes of B. cinerea and C. gloeosporioides have been linked to zoxamide-sensitivity [32], [41], [42], the mechanism of zoxamide-resistance in oomycete pathogens is still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Two Non-target Recessive Genes Confer Resistance to the Anti-Oomycete Microtubule Inhibitor Zoxamide in Phytophthora capsici

Chen

Cai

et al. 2014

PLoS ONE

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This study characterized isolates of P. capsici that had developed a novel mechanism of resistance to zoxamide, which altered the minimum inhibition concentration (MIC) but not the EC50. Molecular analysis revealed that the β-tubulin gene of the resistant isolates contained no mutations and was expressed at the same level as in zoxamide-sensitive isolates. This suggested that P. capsici had developed a novel non-target-site-based resistance to zoxamide. Analysis of the segregation ratio of zoxamide-resistance in the sexual progeny of the sensitive isolates PCAS1 and PCAS2 indicated that the resistance to zoxamide was controlled by one or more recessive nuclear genes. Furthermore, the segregation of resistance in the F1, F2, and BC1 progeny was in accordance with the theoretical ratios of the χ2 test (P>0.05), which suggested that the resistance to zoxamide was controlled by two recessive genes, and that resistance to zoxamide occurred when at least one pair of these alleles was homozygous. This implies that the risk of zoxamide-resistance in P. capsici is low to moderate. Nevertheless this potential for resistance should be monitored closely, especially if two compatible mating types co-exist in the same field.

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Benomyl resistance of Colletotrichum acutatum is caused by enhanced expression of β-tubulin 1 gene regulated by putative leucine zipper protein CaBEN1

Cited by 48 publications

References 54 publications

Sensitivity of Colletotrichum spp. Isolated from Grapes in Korea to Carbendazim and the Mixture of Carbendazim Plus Diethofencarb

Sensitivity of Colletotrichum spp. Isolated from Grapes in Korea to Carbendazim and the Mixture of Carbendazim Plus Diethofencarb

Caracterización química y evaluación de la actividad antifúngica del aceite esencial foliar de Lippia alba contra Colletotrichum gloeosporioides

Two Non-target Recessive Genes Confer Resistance to the Anti-Oomycete Microtubule Inhibitor Zoxamide in Phytophthora capsici

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