Benzimidazole and diphenylamine sensitivity and identity of Penicillium spp. that cause postharvest blue mold of apples using β-tubulin gene sequences

Sholberg, P. L.; Harlton, C. E.; Haag, Paula; Lévesque, C. André; O’Gorman, Daniel T.; Seifert, Keith A.

doi:10.1016/j.postharvbio.2004.07.011

Cited by 57 publications

(34 citation statements)

References 13 publications

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“…Boscalid with pyraclostrobin had shown high efficacy in preharvest application against P. expansum and B. cinerea (Xiao & Boal, 2009). High resistance of several P. expansum isolates has been recorded against benomyl, thiabendazole and diphenylamine (Sholberg et al, 2005). Azoxystrobin, difenoconazole, Polyoxin B and trifloxystrobin have been effective against A. alternata causing core rots (Reuveni & Sheglov, 2002).…”

Section: Fungi Causing Storage Rot Of Apple Fruit In Integrated Pest mentioning

confidence: 99%

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Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

Grantina-Ievina

2015

Rural Sustainability Research

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Abstract. Apple fruit rot can be caused by several fungi. In Northern Europe, the most common storage rot, Bull's eye rot, is caused by Neofabraea spp., bitter rot by Colletotrichum spp., brown rot by Monilinia fructigena, grey mould is caused by Botrytis cinerea and Fusarium rot by several Fusarium species. Blue mold decay caused by Penicillium expansum is an important disease in several European countries. Incidence of different causal agents may vary depending on cultivar, climate during growing season and agricultural practices. The main objective of the study was to obtain baseline information about apple rot-causing fungi, their incidence during fruit storage and to evaluate the fungicide sensitivity of most of isolated fungal species. The study was performed during the storage period of apples after the growth season of 2013. Rotten apples were sorted in the storage and part of them was brought to the laboratory in order to obtain fungal isolates. Fungi were identified according to the morphological characteristics and sequencing of the ITS1-5.8S-ITS2 region. During storage in February and March the total percentage of rotten apples in various cultivars varied from 3.6 to 58.9%. All post-harvest diseases described in Northern Europe were detected. In part of the storehouses apple rot caused by Cadophora luteo-olivacea was observed. Alternaria spp. and Cladosporium spp. were detected on few apples as secondary infection agents. Using the most often isolated fungal species, sensitivity tests were performed against five commonly used fungicides. In general, the sensitivity of tested fungi to the fungicides was high with exception of several Neofabraea and Alternaria isolates.

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Section: Fungi Causing Storage Rot Of Apple Fruit In Integrated Pest mentioning

confidence: 99%

“…P. expansum and P. solitum) were isolated, which are common apple rot-causing pathogens (Sholberg et al, 2005). Isolates of P. brevicompactum have been isolated from rotten apples in Canada (Sholberg & Haag, 1996).…”

Section: Fungi Causing Storage Rot Of Apple Fruit In Integrated Pest mentioning

confidence: 99%

Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

Grantina-Ievina

2015

Rural Sustainability Research

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“…isolates tested. This is not unexpected, since benlate is a member of the beta-tubulin inhibiting class of fungicides, in which thiabendazole (active ingredient in postharvest fungicide Mertect ® ) is routinely used in drenches and dips to control blue mold of apple [26]. It is hypothesized that previous exposure to TBZ, which is used as a postharvest decay control, may have contributed to higher levels (>1 ppm) of tolerance in one isolate of P. expansum and one of P. solitum obtained from decayed apple fruit.…”

Section: Discussionmentioning

confidence: 99%

“…Single spore cultures were preliminarily identified using morphological methods in vitro [25] (Figure 1). Confirmation of species-level identity was achieved using conventional PCR by sequencing ~700 bp of the β-tubulin locus as previously described [26]. Purified PCR products were subjected to Sanger sequencing using both forward and reverse primers.…”

Section: Morphological and Genetic Identification Of Penicillium Sppmentioning

confidence: 99%

“…The majority were isolated from decayed apple fruit with blue mold symptoms from five commercial apple cultivars, one from peach seed, and four from unknown sources. All isolates were identified using morphological and molecular methods as described [25,26]. A ~700 bp portion of the β-tubulin amplicon was subjected to MegaBLAST analysis and, was found to be 99-100% identical with 0.0 E values, to cognate Penicillium spp.…”

Section: Morphological and Genetic Identification Of Penicillium Speciesmentioning

confidence: 99%

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Dominant Selectable Markers for Penicillium spp. Transformation and Gene Function Studies

Jurick¹,

Peng²,

Gaskins³

et al. 2017

Fungal Genom Biol

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Penicillium spp. has been genetically manipulated and gene function studies have utilized single gene deletion strains for phenotypic analysis. Fungal transformation experiments have relied on hygromycin and hygromycin phosphotransferase (hph) as the main dominant selectable marker (DSM) system in Penicillium spp. This poses a limitation on the number of loci that can be analyzed and complemented in reverse genetic studies. Additionally, many economically important Penicillium spp. have not been evaluated to determine the utility of additional chemicals that can serve as DSMs. Therefore, six compounds were examined for 15 blue mold strains and their Minimum Inhibitory Concentrations (MICs) determined. Phleomycin, neomycin and G418 were deemed ineffective, as Penicillium spp. growth was observed on media amended with 1000 μg/ml of each compound. The efficacy of bialophos to inhibit fungal growth was intermediate, with MICs ranging from 250 to 1000 μg/ml and was speciesdependent. However, chlorimuron ethyl and benlate had the lowest MIC values and minimal variation in efficacy within and between species. Therefore, benlate and chlorimuron ethyl are good candidates for use as since corresponding fungal resistance genes have been cloned, characterized and are available from a variety of public and academic sources.

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Quasi‐3D‐Structured Surface‐Enhanced Raman Scattering Substrates Based on Silver Nanoparticles/Mesoporous Silicon Hybrid

Nguyen

Vũ

Pham

et al. 2022

Physica Status Solidi (a)

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The surface‐enhanced Raman scattering (SERS) is a powerful spectroscopic technique for ultrasensitive and selective biochemical detection due to its capability of providing the “fingerprint” of information of molecular structures at low concentrations. Herein, mesoporous silicon (MPSi)‐based nanocomposites containing silver nanoparticles (AgNPs) provide novel efficient substrates for the detection of molecules. The quasi‐3D‐structured SERS substrate is formed by introducing Ag into the MPSi hollow column‐shaped nanostructure. This SERS substrate uniquely combines the ability of metal surfaces to amplify Raman scattering signals with an enlarged surface area and the increasing light–matter interaction duration that generates large SERS signals for the detection of chemicals. The proposed SERS substrate is verified by detecting orange methylene in solutions at ultralow concentration in the range of 10−11–10−6 m with a linear‐dependent coefficient of R2 = 0.99922 and the SERS enhancement factor is achieved up to 1010. Moreover, the proposed platform can detect diphenylamine with ultralow concentration of 10−10 m and reliable reproducibility with an average relative stand deviation of 6.3%. The ability for MPSi substrate to detect target molecules at low concentrations helps pave a way to develop the detection tool for integrated, on‐chip devices.

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Benzimidazole and diphenylamine sensitivity and identity of Penicillium spp. that cause postharvest blue mold of apples using β-tubulin gene sequences

Cited by 57 publications

References 13 publications

Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

Dominant Selectable Markers for Penicillium spp. Transformation and Gene Function Studies

Quasi‐3D‐Structured Surface‐Enhanced Raman Scattering Substrates Based on Silver Nanoparticles/Mesoporous Silicon Hybrid

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