Benzoquinone alters the lipid homeostasis in Saccharomyces cerevisiae

Abstract

Objective: To elucidate the impact of benzoquinone (BQ) on lipid homeostasis and cytotoxicity in Saccharomyces cerevisiae. Methods: The impact of BQ exposure on wild-type and knockouts of PC biosynthesizing genes revealed the alterations in the lipids that were analyzed by fluorescence microscopy, thin layer chromatography, and gene expression studies. Results: In yeast, BQ exposure reduced the growth pattern in wild-type cells. The gene knockout strains of the phospholipid metabolism altered the mRNA expression of the apoptosis genes – both caspase-dependent and independent. The BQ exposure revealed an increase in both the phospholipids and neutral lipids via the CDP:DAG and the Kennedy pathway genes. The accumulation of both neutral lipids and phospholipids during the BQ exposure was discrete and regulated by different pathways. Conclusions: BQ exposure inhibited cell growth, increased the reactive oxygen species (ROS), and altered membrane proliferation. The CDP:DAG and Kennedy pathway lipids also discretely altered by BQ, which is required for the membrane functions and energy purposes of life.

Influence of Nitrogen-Modified Atmosphere Storage on Lipid Oxidation of Peanuts: From a Lipidomic Perspective

“…PC was the most abundant phospholipid, which served as a cell membrane structural component to maintain the homeostasis of intracellular phospholipid content [41,42]. It was noted that the excessive accumulation of ROS led to lipid peroxidation, decreased phospholipid content, and exacerbated cell membrane damage [40].…”

Investigation on the photoinduced chemical reaction between p-benzoquinone and tryptophan in homogeneous solution

“…18 For instance, Raj et al reported that the production rate of ROS increased up to 60% and the cell growth was inhibited in the PBQ environment. 19 As Trp and PBQ play essential roles in a physiological environment, relevant investigations on reactions involving PBQ or Trp have been performed in recent years. [20][21][22][23] However, to the best of our knowledge, the photochemical reaction between them has not been reported.…”

“…18 For instance, Raj et al reported that the production rate of ROS increased up to 60% and the cell growth was inhibited in the PBQ environment. 19…”