Benzothiadiazole as an Inducer of β-1,3-Glucanase and Chitinase Isozymes in Sugar Beet

Burketová, Lenka; Šindelářová, M.; Šindelář, L.

doi:10.1023/a:1002172921426

Cited by 28 publications

(7 citation statements)

References 34 publications

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“…These functional analogs are also known to induce PR proteins/genes [26] , [158] , [159] . In sugar beet, BTH induced accumulation of chitinase and glucanase [160] while in soybean, the chemical up-regulated expression of PR-1, PR-3a, PR-3b, PR-9, and PR- 10 [157] . In banana, exogenous application of BTH caused prolonged expression of chitinase and reduced the symptoms of anthracnose disease [161] .…”

Section: Discussionmentioning

confidence: 97%

Characterization of Withania somnifera Leaf Transcriptome and Expression Analysis of Pathogenesis – Related Genes during Salicylic Acid Signaling

et al. 2014

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Withania somnifera (L.) Dunal is a valued medicinal plant with pharmaceutical applications. The present study was undertaken to analyze the salicylic acid induced leaf transcriptome of W. somnifera. A total of 45.6 million reads were generated and the de novo assembly yielded 73,523 transcript contig with average transcript contig length of 1620 bp. A total of 71,062 transcripts were annotated and 53,424 of them were assigned GO terms. Mapping of transcript contigs to biological pathways revealed presence of 182 pathways. Seventeen genes representing 12 pathogenesis-related (PR) families were mined from the transcriptome data and their pattern of expression post 17 and 36 hours of salicylic acid treatment was documented. The analysis revealed significant up-regulation of all families of PR genes by 36 hours post treatment except WsPR10. The relative fold expression of transcripts ranged from 1 fold to 6,532 fold. The two families of peroxidases including the lignin-forming anionic peroxidase (WsL-PRX) and suberization-associated anionic peroxidase (WsS-PRX) recorded maximum expression of 377 fold and 6532 fold respectively, while the expression of WsPR10 was down-regulated by 14 fold. Additionally, the most stable reference gene for normalization of qRT-PCR data was also identified. The effect of SA on the accumulation of major secondary metabolites of W. somnifera including withanoside V, withaferin A and withanolide A was also analyzed and an increase in content of all the three metabolites were detected. This is the first report on expression patterns of PR genes during salicylic acid signaling in W. somnifera.

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Section: Discussionmentioning

confidence: 97%

Characterization of Withania somnifera Leaf Transcriptome and Expression Analysis of Pathogenesis – Related Genes during Salicylic Acid Signaling

et al. 2014

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“…It was also reported that BTH can stimulate the expression of the defence genes encoding pathogenesis‐related (PR) proteins such as chitinase and β‐1,3‐glucanase in several plants against the attack by pathogens (Lawton et al., 1996; Burketová et al., 1999; Zhu et al., 2003). The two enzymes have direct antifungal activities by degrading cell walls of some fungal species alone or in combination (Ji and Kuć, 1996).…”

Section: Discussionmentioning

confidence: 99%

Postharvest Infiltration of BTH Reduces Infection of Mango Fruits (Mangifera indica L. cv. Tainong) by Colletotrichum gloeosporioides and Enhances Resistance Inducing Compounds

Zhu¹,

Cao²,

Wang³

et al. 2007

Journal of Phytopathology

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Mango fruits (Mangifera indica L.) were treated by vacuum infiltration of 1.0 mm benzo-(1,2,3)-thiadiazole-7-carbothioic acid s-methyl ester (BTH) after harvest. Seventy-two hours after the treatment with BTH, the fruit were inoculated with 15 ll of conidial suspension of Colletotrichum gloeosporioides (1 · 10 5 conidia ⁄ ml) and incubated at 13°C, 85-90% RH for disease development. Disease incidence and lesion diameter in mango fruit after the inoculation were significantly (P < 0.05) reduced by the BTH treatment during the incubation. Peroxidase, polyphenoloxidase, phenylalanine ammonia-lyase, chitinase and b-1,3-glucanase activities and total phenolic compounds content in the fruits were all enhanced by the BTH treatment during the incubation. The catalase activity in the fruit was inhibited, whereas the level of hydrogen peroxide was increased by the BTH treatment during the infection. These responses may be involved in the induced resistance against the pathogen infection in mango fruit by BTH treatment. Application of BTH in fruit possesses promising results in the control of postharvest diseases as an alternative to traditional methods.

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“…Other protein changes are more directly involved in abiotic stress tolerance mechanisms such as heat shock proteins (HSPs) or dehydrins that play protective roles during stress events ( Wang et al, 2004 ; Hanin et al, 2011 ). Most changes in protein accumulation due to ASM treatment have been studied in response to pathogen infection and have focused on proteins involved in defense against biotic stresses, finding increased accumulations of pathogenesis related proteins and anti-oxidant proteins such as chitinases, 1,3-betaglucanases, and peroxidases ( Burketová et al, 1999 ; Narusaka et al, 1999 ; Brisset et al, 2000 ; Cao and Jiang, 2006 ). Several proteomic studies have also identified changes to defense proteins such as HSPs, anti-oxidant, and energy proteins, including ATP synthase and Rubisco in response to ASM treatment ( Barilli et al, 2012 ; Gunel et al, 2012 ; Chen et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass

Jespersen

Huang

2017

Front. Plant Sci.

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Acibenzolar-S-methyl (ASM) is a synthetic functional analog of salicylic acid which can induce systemic acquired resistance in plants, but its effects on abiotic stress tolerance is not well known. The objectives of this study were to examine effects of acibenzolar-S-methyl on heat or drought tolerance in creeping bentgrass (Agrostis stolonifera) and to determine major ASM-responsive metabolites and proteins associated with enhanced abiotic stress tolerance. Creeping bentgrass plants (cv. ‘Penncross’) were foliarly sprayed with ASM and were exposed to non-stress (20/15°C day/night), heat stress (35/30°C), or drought conditions (by withholding irrigation) in controlled-environment growth chambers. Exogenous ASM treatment resulted in improved heat or drought tolerance, as demonstrated by higher overall turf quality, relative water content, and chlorophyll content compared to the untreated control. Western blotting revealed that ASM application resulted in up-regulation of ATP synthase, HSP-20, PR-3, and Rubisco in plants exposed to heat stress, and greater accumulation of dehydrin in plants exposed to drought stress. Metabolite profiling identified a number of amino acids, organic acids, and sugars which were differentially accumulated between ASM treated and untreated plants under heat or drought stress, including aspartic acid, glycine, citric acid, malic acid, and the sugars glucose, and fructose. Our results suggested that ASM was effective in improving heat or drought tolerance in creeping bentgrass, mainly through enhancing protein synthesis and metabolite accumulation involved in osmotic adjustment, energy metabolism, and stress signaling.

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Benzothiadiazole as an Inducer of β-1,3-Glucanase and Chitinase Isozymes in Sugar Beet

Cited by 28 publications

References 34 publications

Characterization of Withania somnifera Leaf Transcriptome and Expression Analysis of Pathogenesis – Related Genes during Salicylic Acid Signaling

Characterization of Withania somnifera Leaf Transcriptome and Expression Analysis of Pathogenesis – Related Genes during Salicylic Acid Signaling

Postharvest Infiltration of BTH Reduces Infection of Mango Fruits (Mangifera indica L. cv. Tainong) by Colletotrichum gloeosporioides and Enhances Resistance Inducing Compounds

Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass

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