Benzotriazole Enhances Cell Invasive Potency in Endometrial Carcinoma Through CTBP1-Mediated Epithelial-Mesenchymal Transition

Abstract

Background/Aims: Benzotriazole (BTR) and its derivatives, such as intermediates and UV stabilizers, are important man-made organic chemicals found in everyday life that have been recently identified as environmental toxins and a threat to female reproductive health. Previous studies have shown that BTR could act as a carcinogen by mimicking estrogen. Environmental estrogen mimics could promote the initiation and development of female cancers, such as endometrial carcinoma, a type of estrogenic-sensitive malignancy. However, there is little information on the relationship between BTR and endometrial carcinoma. In this study, we aimed to demonstrate the biological function of BTR in endometrial carcinoma and explored the underlying mechanism. Methods: The CCK-8 assay was performed to detect cell viability; transwell-filter assay was used to assess cell invasion; gene microarray analysis was employed to determine gene expression patterns in response to BTR treatment; western blotting and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were carried out to detect the expression levels of BTR-related genes. Results: Our data showed that BTR could induce the invasion and migration of endometrial carcinoma cells (Ishikawa and HEC-1-B). In addition, BTR increased the expression level of CTBP1, which could enhance the epithelial-mesenchymal transition (EMT) in cancer cells. Moreover, CTBP1 silencing reversed the effect of BTR on EMT progression in endometrial carcinoma cells. Conclusion: This study indicates that BTR could act as a carcinogen to promote the development of endometrial carcinoma mainly through CTBP1-mediated EMT, which deserves more attention.

The possible role furin and furin inhibitors in endometrial adenocarcinoma: A narrative review

“…ADAM17 is activated by PC furin with subsequent induction release of TNF‐α 75 . Higher ADAM17 expression is linked with the development of EAC 77 . Xu and coworkers found that a high level of ADAM17 was associated with poor clinical outcomes in women with uterine carcinoma 78 .…”

Novel mulberry silkworm cocoon-derived carbon dots and their anti-inflammatory properties

“…The cytotoxicity profiles of the MSC-CDs were studied in RAW 264.7 cells using a CCK-8 assay [26]. The cells were cultured in Dulbecco's modified Eagle's medium supplemented with 20% foetal bovine serum in a humidified 5% CO 2 atmosphere at 37 C. The RAW 264.7 cells were counted and seeded in 96-well plates at a density of 1 Abs sample and Abs control represent the A450 of the experimental and control groups, respectively.…”

Degradation of benzotriazole and benzothiazole with the UV-activated peracetic acid process: performance, mechanism and transformation pathway

“…3 These chemicals are ineffectively removed by traditional wastewater treatment plant (WWTP) processes [4][5][6][7][8] and have adverse impacts (e.g., endocrine disrupting effects) on aquatic organisms according to in vitro and in vivo tests; 3,9,10 additionally, benzotriazoles exhibit carcinogenicity and genotoxicity. 11,12 Among all benzotriazoles and benzothiazoles, benzotriazole (BT) and benzothiazole (BTH) are the two representative examples. The intrinsic physicochemical profiles of BT and BTH are presented in Table S1.…”