�ber das Wasserstoff aktivierende System von Hydrogenomonas H16

Eberhardt, Ulrich

doi:10.1007/bf00446675

Cited by 29 publications

(4 citation statements)

References 32 publications

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“…Hydrogen is used in the oxygen-hydrogen reaction serving the generation of ener~ (equation 1) and in the fixation of CO 2 to the level of cellular material (equation 2). The ratio of hydrogen uptake over the uptake of carbon dioxide (H2/CO 2) by a suspension of growing cells is dependent on the efficiency of coupling, on the expenditure for maintenance energy and on the level, at which the electrons enter the respiratory chain; HJCO 2 ratios of 4…”

Section: Discussionmentioning

confidence: 99%

“…The one is a NAD-reducing, soluble enzyme (H~ : N,~D oxidoreductase) and the other is a membrane-bound enzyme unable to reduce pyridine nucleotides. With respect to the distribution of both types of hydrogenases three groups of hydrogen-oxidizing bacteria can be differentiated (table I) : (i) Alcaligenes eutrophus [4], A. ruhlandii, [5] and Pseudomonas saccharophila [6] contain both types, the soluble and the membrane-hound hydrogenase ; (it) Nocardia opaca lb (7) and some further species of the genus Nocardia contain a soluble, NAD-reducing enzyme only; (iii) the majority of the hydrogen-oxidizing bacteria, such as Alcaligenes latus, Aquaspirillum aulolrophicum, Paracoccus den itri[icans, several pseudomonads and the coryneform nitrogen-fixing strains, contain only a membrane-bound hydrogenase [8].…”

Section: Hydrogenases Their Distribulion and Regulationmentioning

confidence: 99%

“…The presence of two hydrogenases has been described for Pseudomonas saccharophila [6~, Alcaligenes ruhlandii [15; 5] and Alcaligenes eutrophus [4]. The soluble enzyme has been recently purified and characterized.…”

Section: Seliberia Carboxgdohgdrogenamentioning

confidence: 99%

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Hydrogen metabolism in aerobic hydrogen-oxidizing bacteria

Schink¹,

Schlegel²

1978

Biochimie

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A survey on organisms able to use molecular hydrogen as electron donor in the energy-yielding process is presented. In the group of the aerobic hydrogen-oxidizing bacteria so far two types of hydrogenases have been encountered, a NAD-reducing, soluble enzyme (H2 : NAD oxidoreductase) and a membrane-bound enzyme unable to reduce pyridine nucleotides. With respect to the distribution of both types of hydrogenases three groups of hydrogen-oxidizing bacteria can be diffentiated containing (i) both types (Alcaligenes eutrophus), (ii) a soluble enzyme only (Nocardia opaca lb), and (iii) a membrane-bound hydrogenase only (majority of genera and species). The results of studies on the NAD-specific hydrogenase of A. eutrophus are summarized. Results on the solubilization and purification of the membrane-bound hydrogenase of A. eutrophus are presented in detail. The enzyme was solubilized from purified membranes by Triton X-100 and sodium desoxycholate or phospholipase D. The crude membrane extract was fractionated by ammonium sulfate precipitation and chromatography on carboxymethylcellulose at pH 5.5. The enzyme was stable in potassium phosphate buffer; it resembles the soluble enzyme with respect to stability under oxidizing conditions. Further biochemical and immunological data indicate, however, that both enzymes are different with respect to their native structure.

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Section: Discussionmentioning

confidence: 99%

Section: Hydrogenases Their Distribulion and Regulationmentioning

confidence: 99%

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Hydrogen metabolism in aerobic hydrogen-oxidizing bacteria

Schink¹,

Schlegel²

1978

Biochimie

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“…In A. eutrophus [21] and A. ruhlandii [22], both the cytoplasmic fraction and the particulate fraction of the crude sonicate of the cells separated by centrifugation contained hydrogenase activity [16]. When analogous methods were used and malate dehydrogenase and NADH oxidase were measured as reference enzyme activities, essentially no hydrogenase activity was detected in the soluble, cytoplasmic fraction of P. saccharophila.…”

Section: Localization Of Hydrogenase Activitymentioning

confidence: 99%