�ber den Mechanismus der blutgerinnungshemmenden Wirkung des Tabanins

Anticoagulant activities in salivary glands of tabanid flies

“…In general, the antihaemostatic strategies of higher Diptera seem to differ from blood‐feeding species in lower Diptera (Hudson, 1964; Cupp et al ., 1998) and are known to include factors that mainly target thrombin and inhibit platelet aggregation, e.g. in Glossinidae (Mant & Parker, 1981; Cappello et al ., 1998), horn flies (Cupp et al ., 2000), horseflies and deerflies (Markwardt & Schulz, 1960; Reddy et al ., 2000).…”

“…Indeed, females of the larger species are able to ingest up to 200 mg of blood within only 1–3 min (Chvála et al ., 1980) suggesting that they must possess very potent anticlotting mechanisms. Potent antithrombin activity in the salivary glands of tabanids was noted during the discovery of tabanin, a thrombin inhibitor from Tabanus bovinus (Markwardt & Leberecht, 1959; Markwardt & Schulz, 1960). Recently, a platelet aggregation inhibitor and a potent glycoprotein IIb/IIIa fibrinogen receptor antagonist have been isolated from the deerfly Chrysops sp.…”

Identification of Anticoagulant Activities in Salivary Gland Extracts of Four Horsefly Species (Diptera, Tabanidae)

“…[9,10]. Apart from the discovery of tabanin, a potent inhibitor of thrombin [4,7,8] in one of the largest species (Tabanus bovinus) which shows similarities to the mode of action of hirudin, horsefly anticoagulants have not been studied. Our results confirmed the existing knowledge on horsefly anticoagulants in routine coagulation assays (TT, PT, APTT).…”

“…Potent anticoagulant activity was discovered in the salivary glands of some representatives of the Tabaninae subfamily, which was attributed to antithrombin [7]. A thrombin inhibitor, isolated from Tabanus bovinus, was named tabanin [8]. Tabanin is thought to be an approximately 7-kD peptide [4]; however, its amino acid sequence is unknown.…”

Effects of horsefly (Tabanidae) salivary gland extracts on isolated perfused rat heart

“…Potent antithrombin activities have been observed in the salivary glands (SGs) of the horsefly Tabanus bovinus Linnaeus (Markwardt & Schulz, 1960) and an inhibitor of platelet aggregation was isolated from salivary gland extracts (SGEs) of deerflies (genus Chrysops ) (Grevelink et al , 1993). Anticoagulant activities were also identified in crude and partially purified SGEs of four species from the Tabaninae subfamily, namely, Tabanus autumnalis Linnaeus, Hybomitra muehlfeldi Brauer, Haematopota pluvialis Linnaeus and Heptatoma pellucens Fabricius (Kazimírová et al ., 2001).…”