�ber die selektive fermentcytochemische Darstellung von neutrophilen myeloischen Zellen und Gewebsmastzellen im Paraffinschnitt

Leder, L. D.

doi:10.1007/bf01486688

Cited by 345 publications

(51 citation statements)

References 3 publications

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“…Five-micrometer tissue sections were cut from the caudal part of the third slice and stained with hematoxylin-eosin and toluidine blue, a standard metachromatic histopathological technique to detect the heparin-containing granules present only in MCs ( Figure ID in the online-only Data Supplement), and stained with chloroacetate esterase (Leder) 26 to also detect polymorphonuclear neutrophils and erythrocytes ( Figure IA and IE to IG in the online-only Data Supplement). Light microscopy was performed by an experienced hematopathologist (M.-L.K.-L.) with an Olympus BH-2 device, followed by photography with a Nikon Eclipse E600 microscope connected to a digital camera (Nikon Coolpix 995).…”

Section: Histopathological Examinationsmentioning

confidence: 99%

Mast Cell Stabilization Reduces Hemorrhage Formation and Mortality After Administration of Thrombolytics in Experimental Ischemic Stroke

Strbian

Karjalainen–Lindsberg²,

Kovanen³

et al. 2007

Circulation

101

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Background-Thrombolysis with tissue plasminogen activator (tPA) improves stroke outcome, but hemorrhagic complications and reperfusion injury occasionally impede favorable prognosis after vessel recanalization. Perivascularly located cerebral mast cells (MCs) release on degranulation potent vasoactive, proteolytic, and fibrinolytic substances. We previously found MCs to increase ischemic and hemorrhagic brain edema and neutrophil accumulation. This study examined the role of MCs in tPA-mediated hemorrhage formation (HF) and reperfusion injury. Methods and Results-Exposure to tPA in vitro induced strong MC degranulation. In vivo experiments in a focal cerebral ischemia/reperfusion model in rats showed 70-to 100-fold increase in HF after postischemic tPA administration (PϽ0.001). Pharmacological MC stabilization with cromoglycate led to significant reduction in tPA-mediated HF at 3 (97%), 6 (76%), and 24 hours (96%) compared with controls (PϽ0.01, PϽ0.001, and PϽ0.01, respectively). Furthermore, genetically modified MC-deficient rats showed similarly robust reduction of tPA-mediated HF at 6 (92%) and 24 (89%) hours compared with wild-type littermates (PϽ0.01 and PϽ0.001, respectively). MC stabilization and MC deficiency also significantly reduced other hallmarks of reperfusion injury, such as brain swelling and neutrophil infiltration. These effects of cromoglycate and MC deficiency translated into significantly better neurological outcome (PϽ0.01 and PϽ0.05, respectively) and lower mortality (PϽ0.05 and PϽ0.05, respectively) after 24 hours. Conclusions-MCs appear to play an important role in HF and reperfusion injury after tPA administration. Pharmacological stabilization of MCs could offer a novel type of therapy to improve the safety of administration of thrombolytics.

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Section: Histopathological Examinationsmentioning

confidence: 99%

Mast Cell Stabilization Reduces Hemorrhage Formation and Mortality After Administration of Thrombolytics in Experimental Ischemic Stroke

Strbian

Karjalainen–Lindsberg²,

Kovanen³

et al. 2007

Circulation

101

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“…A 4-mm-thick section of tissue encompassing the lesion was taken from each brain and embedded in paraffin. Ten micron sections were taken with a microtome and stained with Perl's Prussian blue for ferric iron and Leder's stain (Leder, 1964) for chloracetate esterase activity (Kit 91-C, Sigma, Oakville, ONT, Canada). These particular stains have been used to quantify components of inflammation after ICH in several other studies (Del Bigio et al, 1996;Peeling et al, 2001).…”

Section: Forelimb Use Asymmetry Test (Experiments 1 and 2)mentioning

confidence: 99%

Mild to Moderate Hyperthermia Does Not Worsen Outcome after Severe Intracerebral Hemorrhage in Rats

MacLellan

Colbourne

2005

J Cereb Blood Flow Metab

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Hyperthermia worsens outcome in clinical and experimental studies of ischemic stroke. Thus, we tested whether hyperthermia aggravates intracerebral hemorrhage (ICH) in rats. A striatal hemorrhage was produced via an infusion of bacterial collagenase. In a preliminary experiment, we compared brain and core temperatures (via telemetry) during heating (infrared lamp). The brain temperature rise exceeded that produced by enforced core hyperthermia, which was used subsequently. In these experiments up to three hyperthermia conditions (versus normothermia) were tested including: hyperthermia (438.51C) over the first (HYP-1) or second 24 h period (HYP-2) after ICH and 3 h of 401C hyperthermia starting 12 h after ICH (HYP-3). The HYP-1, HYP-2, and HYP-3 treatments did not affect functional deficits (e.g., spontaneous forelimb use, skilled reaching) or the volume of injury at 30 days. Furthermore, the HYP-1 treatment did not aggravate injury or deficits at 7 days. Bleeding and inflammation, which contribute to pathology, were not significantly altered by HYP-1 and HYP-3 treatments. Bleeding was assessed at 1 day, and macrophages and neutrophils were counted at 2 and 4 days. Accordingly, hyperthermia, under the present conditions, did not worsen outcome after striatal ICH.

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“…To specifically stain granulocytes, naphthol-AS-D-chloroacetate esterase staining was performed according to the standard protocol of Leder (38).…”

Section: Histological Analysismentioning

confidence: 99%

CD95 Ligand-Expressing Tumors Are Rejected in Anti-Tumor TCR Transgenic Perforin Knockout Mice

Behrens

Igney

Arnold

et al. 2001

The Journal of Immunology

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CD95 (APO-/Fas) ligand (CD95L) is a member of the TNF family predominantly expressed by activated T and NK cells but also by tumors of diverse cellular origin. CD95L trimerizes surface CD95 expressed by target cells that subsequently undergo apoptosis. The role of the CD95/CD95L system in the down-regulation of an immune response (activation-induced cell death) is established. However, it is so far unclear why tumors express CD95L. To investigate whether tumors use the CD95L to down-regulate an anti-tumor immune response, we established a transgenic (tg) mouse model consisting of 1) apoptosis-resistant tumor cells, designated LKC-CD95L, which express functional CD95L and the model tumor Ag Kb; and 2) perforin knockout (PKO) anti-Kb TCR tg mice. L1210-Fas antisense expressing Kb, crmA, and CD95L (LKC-CD95L) killed CD95+ unrelated tumor targets and Con A-activated splenocytes from anti-Kb TCR tg PKO mice by a CD95L-dependent mechanism in vitro. However, we could not detect any cytotoxic activity against anti-tumor (anti-Kb) T cells in vivo. We also observed reduced growth of LKC-CD95L in nude mice and rapid rejection in anti-Kb TCR tg PKO mice. Because the tumor cells are resistant to CD95L-, TNF-α-, and TNF-related apoptosis-inducing ligand-induced apoptosis and the mice used are perforin-deficient, the involvement of these four cytotoxicity mechanisms in tumor rejection can be excluded. The histological examination of tumors grown in nude mice showed infiltration of LKC-CD95L tumors by neutrophils, whereas L1210-Fas antisense expressing Kb and crmA (LKC) tumor tissue was neutrophil-free. Chemotaxis experiments revealed that CD95L has no direct neutrophil-attractive activity. Therefore, we conclude that LKC-CD95L cells used an indirect mechanism to attract neutrophils that may cause tumor rejection.

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�ber die selektive fermentcytochemische Darstellung von neutrophilen myeloischen Zellen und Gewebsmastzellen im Paraffinschnitt

Cited by 345 publications

References 3 publications

Mast Cell Stabilization Reduces Hemorrhage Formation and Mortality After Administration of Thrombolytics in Experimental Ischemic Stroke

Mast Cell Stabilization Reduces Hemorrhage Formation and Mortality After Administration of Thrombolytics in Experimental Ischemic Stroke

Mild to Moderate Hyperthermia Does Not Worsen Outcome after Severe Intracerebral Hemorrhage in Rats

CD95 Ligand-Expressing Tumors Are Rejected in Anti-Tumor TCR Transgenic Perforin Knockout Mice

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