Ber-EP4 staining in effusion cytology: A potential source of false positives

Arco, Cristina Díaz del; Sanz, Melchor Sáiz-Pardo; Ortega, Luís; Fernández-Aceñero, María Jesús

doi:10.1016/j.patol.2020.04.005

Cited by 6 publications

(3 citation statements)

References 61 publications

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“…A D2‐40 membranous staining pattern can also be seen in some cases of carcinomas originating in the ovary, lung or breast 15 . Ber‐EP4 staining can be seen in mesothelial cells; this staining is primarily weak and patchy but in some cases may be strong 16 …”

Section: Discussionmentioning

confidence: 99%

“…15 Ber-EP4 staining can be seen in mesothelial cells; this staining is primarily weak and patchy but in some cases may be strong. 16 In cases of metastatic adenocarcinoma the most frequently encountered primary sites are the lung, ovary, colon, breast, stomach, pancreas, and bile duct. 17 There is no single antibody that can be used as a magic bullet, but a combinatorial immunostaining panel is beneficial.…”

Section: Discussionmentioning

confidence: 99%

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Identifying the primary site of malignancy in carcinomatous serous effusions using immunocytochemistry

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identifying the primary site of malignancy in carcinomatous serous effusions using immunocytochemistry

et al. 2022

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“…Although certain examples, there are recorded cases of mesothelium with BER-EP4, although the majority of these patients only displayed little or undetectable staining of the mesothelium. However, in rare instances, cytological and histological specimens may be strongly positive (12) . About 85% of lung adenocarcinoma and thyroid cancer patients express TTF-1 positively.…”

Section: Table (2)mentioning

confidence: 98%

Role of Immunohistochemistry in Pleural Effusion Cytology: Review Article

2023

The Egyptian Journal of Hospital Medicine

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Background: Immunohistochemistry is crucial for the proper diagnosis of malignant mesothelioma, especially in cases with complex morphology and in biopsy and cytology specimens where it is difficult or impossible to determine the tumor architecture. In the vast majority of instances, the correct identification of tumor lineage is made possible by the application of a tailored panel of mesothelial-and epithelial-specific markers. Methods: The authors review the most commonly used cytologic preparations, fixatives, and antibodies used in effusion ICC. Results: Through the utilization of cell block preparations and a panel of antibodies appropriate for the differential diagnosis in question, ICC conditions utilized in surgical pathology can be most closely replicated. Conclusions: Accurate differentiation between malignant mesothelioma and lung adenocarcinoma in pleural effusion cytology is still a big challenge. Therefore, it is mandatory to search for new diagnostic immunohistochemical markers.

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Lavage cytology diagnosed by immunostaining may be a poor prognostic factor in pathological stage III colorectal cancer

Nankaku,

Yamaoka,

Shiomi

et al. 2024

Annals of Gastroent Surgery

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AimTo clarify the prognostic impact of positive lavage cytology diagnosed by immunostaining on long‐term outcomes following curative resection for pathological stage III colorectal cancer (CRC).MethodWe retrospectively investigated patients who underwent radical resection and intraoperative lavage cytology (LCY) simultaneously for pathological stage III primary CRC between 2005 and 2017. All LCY specimens were evaluated by Papanicolaou staining and immunostaining for carcinoembryonic antigen and Ber‐EP4. Only Class V diagnosed by either staining method was defined as positive LCY, and patients were classified into two groups: a positive lavage cytology (LCY+) group; and a negative lavage cytology (LCY−) group. Overall survival (OS) and relapse‐free survival (RFS) were compared between groups. Multivariate analysis was performed to identify clinicopathological factors affecting OS and RFS.ResultsAmong 708 patients with pathological stage III CRC, 30 patients (4.2%) showed positive LCY. OS and RFS were significantly lower in the LCY(+) group than in the LCY(−) group. Five‐y OS rates in the LCY(+) and LCY(−) groups were 58.7% and 91.0%, respectively, and 5‐y RFS rates were 28.8% and 76.6%, respectively. Multivariate analysis revealed that positive LCY was independently associated with lower OS and RFS. In the LCY(+) group, the proportion of patients with negative Papanicolaou staining but positive immunostaining was 20.0% (6 of 30). No significant differences in OS and RFS were evident between those patients and patients with positive results for both Papanicolaou staining and immunostaining.ConclusionPositive LCY as diagnosed by immunostaining may represent a poor prognostic factor for pathological stage III CRC.

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Ber-EP4 staining in effusion cytology: A potential source of false positives

Cited by 6 publications

References 61 publications

Identifying the primary site of malignancy in carcinomatous serous effusions using immunocytochemistry

Identifying the primary site of malignancy in carcinomatous serous effusions using immunocytochemistry

Role of Immunohistochemistry in Pleural Effusion Cytology: Review Article

Lavage cytology diagnosed by immunostaining may be a poor prognostic factor in pathological stage III colorectal cancer

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