Berberine affords protection against oxidative stress and apoptotic damage in F1 generation of wistar rats following lactational exposure to chlorpyrifos

Seth, Era; Ahsan, Aitizaz Ul; Kaushal, Sakshi; Mehra, Sweety; Chopra, Mani

doi:10.1016/j.pestbp.2021.104977

Cited by 16 publications

(6 citation statements)

References 58 publications

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“…Studies have confirmed that CPF exposure in pregnant rats or newborn mice triggers impaired central nervous development, and even affects neuronal axonal transport and neuronal function. [28] In addition, longterm exposure to CPF significantly increases the risk of Parkinson's and other neurodegenerative diseases. [29][30][31] Slow movement, reduced food intake, and weight loss are the basic biological characteristics of neurodegenerative diseases in rodents.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the effect of ginsenoside Rg1 on CPF‐induced brain injury in mice via PI3k/AKT pathway

Liu

Pei

Chen

et al. 2023

J Biochem & Molecular Tox

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Organophosphorus pesticides (OPs) have long been used extensively on agricultural land and can lead to significant improvements in crop yields. Due to occupational exposure, humans are exposed to pesticides through dermal contact, inhalation, and ingestion. The effects of OPs on the organism are currently studied for their effects on livers, kidneys, hearts, blood indicators, neurotoxicity, and teratogenic, carcinogenic, and mutagenic effects, while studies in the direction of brain tissue damage have not been reported in detail. Previous reports have confirmed that ginsenoside Rg1 is a prominent and representative tetracyclic triterpenoid derivative rich in ginseng and has good neuroprotective activity. Considering that, the aim of this study was to establish a mouse model of brain tissue injury by using the OP-type pesticide chlorpyrifos (CPF) and to explore the therapeutic effects and possible molecular mechanisms of Rg1. Mice in the experimental group were pre-protected with Rg1 by gavage for 1 week, and brain tissue damage was induced using CPF (5 mg/kg for 1 week) to assess the effect of Rg1 (80 and 160 mg/kg for 3 weeks) in alleviating brain damage. Morris water maze and histopathological analysis were performed to assess cognitive function and pathological changes in the mouse brain, respectively. Protein expression levels of Bax, Bcl-2, Caspase-3, Cl-Cas-3, Caspase-9, Cl-Cas-9, phosphoinositide 3-kinase (PI3K), phosphorylated-PI3K, protein kinase B (AKT), and phosphorylated-AKT were quantified by protein blotting analysis. Rg1 obviously restored CPF-induced oxidative stress damage in mouse brain tissue, increased the levels of antioxidant parameters (total superoxide dismutase, total antioxidative capacity, and glutathione) in the brain, and significantly reduced the overexpression of apoptosis-related proteins induced by CPF. At the same time, Rg1 also markedly attenuated the histopathological changes in the brain induced by CPF exposure. Mechanistically, Rg1 could effectively activate the phosphorylation of PI3K/AKT. Furthermore, molecular docking studies revealed a stronger binding capacity between Rg1 and PI3K. Rg1 attenuated neurobehavioural alterations and reduced lipid peroxidation in the mouse brain to a great extent. Apart from that, Rg1 administration improved brain histopathology in CPF-induced rats. All results suggest that ginsenoside Rg1 has potential antioxidant effects on CPF-induced

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Section: Discussionmentioning

confidence: 99%

Evaluating the effect of ginsenoside Rg1 on CPF‐induced brain injury in mice via PI3k/AKT pathway

Liu

Pei

Chen

et al. 2023

J Biochem & Molecular Tox

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“…Some previous studies have also reported that BBR is effective against oxidative stress-mediated bimolecular alterations following doxorubicin [31] and chlorpyrifos exposure. [55,56] Endogenous antioxidants are cellular tools for defense against disrupted redox balance and oxidative damage to lipids and proteins.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective potential of berberine against acetamiprid induced toxicity in rats: Implication of oxidative stress, mitochondrial alterations, and structural changes in brain regions

Phogat

Singh

Malik

et al. 2023

J Biochem & Molecular Tox

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Acetamiprid (ACMP) is an extensively used neonicotinoid pesticide to control sucking and chewing insects and is known to cause nontarget toxicity. The present study aimed to evaluate the ameliorative potential of berberine (BBR)—a polyphenolic alkaloid‐ on ACMP‐induced oxidative stress, mitochondrial dysfunctioning, and structural changes in different rat brain regions. The male Wistar rats were divided into four groups, that is, control, BBR‐treated (150 mg/kg b.wt), ACMP‐exposed (21.7 mg/kg b.wt) and BBR + ACMP co‐treated; and were dosed intragastrically for 21 consecutive days. Results of the biochemical analysis showed that BBR significantly ameliorated ACMP‐induced oxidative stress by decreasing lipid peroxidation and protein oxidation along with a marked increase in endogenous antioxidants and lowered AChE activity in rat brain regions. Inside mitochondria, BBR significantly attenuated the toxic effects of ACMP by increasing the activity of mitochondrial complexes. Findings of polymerase chain reaction also demonstrated the modulatory effects of BBR against ACMP‐mediated downregulation of ND1, ND2, COX1, and COX4 subunits of mitochondrial complexes. The histopathological and ultrastructural examination also validated the biochemical and transcriptional alterations following toxicity of ACMP exposure and the protective potential of BBR against ACMP‐induced neurotoxicity. Thus, the present study indicates the promising ameliorative potential of BBR against ACMP‐induced neurotoxicity via its antioxidative and modulatory activities.

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“…Berberine (BBR) is an isoquinoline alkaloid found in the long-used traditional Chinese herbs Rhizomacoptidis, Cortex Phellodendri, and Mahonia bealei. BBR reportedly has anti-inflammatory ( Li Y. et al, 2022 ; Dai et al, 2022 ) and antioxidant properties ( Cao et al, 2021 ; Seth et al, 2021 ). BBR has therapeutic effects on various diseases, including osteoarthritis.…”

Section: Phytochemicals For the Treatment Of Idd By Targeting Inflamm...mentioning

confidence: 99%

Targeting Oxidative Stress and Inflammation in Intervertebral Disc Degeneration: Therapeutic Perspectives of Phytochemicals

et al. 2022

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Low back pain is a major cause of disability worldwide that declines the quality of life; it poses a substantial economic burden for the patient and society. Intervertebral disc (IVD) degeneration (IDD) is the main cause of low back pain, and it is also the pathological basis of several spinal degenerative diseases, such as intervertebral disc herniation and spinal stenosis. The current clinical drug treatment of IDD focuses on the symptoms and not their pathogenesis, which results in frequent recurrence and gradual aggravation. Moreover, the side effects associated with the long-term use of these drugs further limit their use. The pathological mechanism of IDD is complex, and oxidative stress and inflammation play an important role in promoting IDD. They induce the destruction of the extracellular matrix in IVD and reduce the number of living cells and functional cells, thereby destroying the function of IVD and promoting the occurrence and development of IDD. Phytochemicals from fruits, vegetables, grains, and other herbs play a protective role in the treatment of IDD as they have anti-inflammatory and antioxidant properties. This article reviews the protective effects of phytochemicals on IDD and their regulatory effects on different molecular pathways related to the pathogenesis of IDD. Moreover, the therapeutic limitations and future prospects of IDD treatment have also been reviewed. Phytochemicals are promising candidates for further development and research on IDD treatment.

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Berberine affords protection against oxidative stress and apoptotic damage in F1 generation of wistar rats following lactational exposure to chlorpyrifos

Cited by 16 publications

References 58 publications

Evaluating the effect of ginsenoside Rg1 on CPF‐induced brain injury in mice via PI3k/AKT pathway

Evaluating the effect of ginsenoside Rg1 on CPF‐induced brain injury in mice via PI3k/AKT pathway

Neuroprotective potential of berberine against acetamiprid induced toxicity in rats: Implication of oxidative stress, mitochondrial alterations, and structural changes in brain regions

Targeting Oxidative Stress and Inflammation in Intervertebral Disc Degeneration: Therapeutic Perspectives of Phytochemicals

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