Berberine ameliorates the LPS-induced imbalance of osteogenic and adipogenic differentiation in rat bone marrow-derived mesenchymal stem cells

Abstract

Lipopolysaccharide (LPS) from oral pathogenic bacteria is an important factor leading to alveolar bone absorption and the implant failure. The present study aimed to evaluate the modulation of berberine hydrochloride (BBR) on the LPS-mediated osteogenesis and adipogenesis imbalance in rat bone marrow-derived mesenchymal stem cells (BMSCs). Cell viability, osteoblastic and adipogenic differentiation levels were measured using the Cell Counting Kit-8 assay, alkaline phosphatase (ALP) staining and content assay, and oil red O staining, respectively. Reverse transcription-quantitative PCR and immunoblotting were used to detect the related gene and protein expression levels. In undifferentiated cells, BBR increased the mRNA expression levels of the osteoblastic genes (Alp, RUNX family transcription factor 2, osteocalcin and secreted phosphoprotein 1) but not the adipogenic genes (fatty acid binding protein 4, Adipsin and peroxisome proliferator-activated receptorγ). LPS-induced osteoblastic gene downregulation, adipogenic gene enhancement and NF-κB activation were reversed by BBR treatment. In osteoblastic differentiated cells, decreased ALP production by LPS treatment was recovered with BBR co-incubation. In adipogenic differentiated cells, LPS-mediated lipid accumulation was decreased by BBR administration. The mRNA expression levels of the pro-inflammatory factors (MCP-1, TNF-α, IL-6 and IL-1β) were increased by LPS under both adipogenic and osteoblastic conditions, which were effectively ameliorated by BBR. The actions of BBR were attenuated by compound C, suggesting that the role of BBR may be partly due to AMP-activated protein kinase activation. The results demonstrated notable pro-osteogenic and anti-adipogenic actions of BBR in a LPS-stimulated inflammatory environment. This indicated a potential role of BBR for bacterial infected-related peri-implantitis medication.

An all-silk-derived bilayer hydrogel for osteochondral tissue engineering

“…BBR has been widely studied due to its anti-inflammatory, antioxidant, antibacterial and immune regulation properties [ [51] , [52] , [53] ]. Recent studies have shown that BBR can promote the osteogenesis of BMSCs [ 27 , 28 , 32 ]. Similar to KGN, the stable molecular structure of BBR facilitates in vitro manipulation and encapsulation by various materials and maintains biological activity in the control release process.…”

“…Berberine (BBR) has been reported to induce BMSCs osteogenesis and inhibit OA progression in animal models [ [27] , [28] , [29] , [30] ]. Moreover, the antioxidant and anti-inflammatory properties of BBR are expected to inhibit oxidative stress-induced apoptosis and maintain the differentiation potentials of BMSCs under the inflammatory environment of injured joints [ 31 , 32 ]. Besides, the hydrophobicity of KGN and BBR allows them to be efficiently encapsulated in SF nano- and microspheres using a simple, rapid and scalable method described by Wang et al., thereby realizing long-term sustained release [ 33 , 34 ].…”

Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate

“…For adipogenic differentiation, MSCs were incubated in a DMEM supplemented with 1 µM dexamethasone, 0.5 mM isobutylmethylxanthine, 10 nM insulin and 100 µM indomethacin at 37 °C for 12 days, and the media were changed every 3 days. Then, the cells were harvested for oil red O staining [ 30 , 31 ].…”

Galangin inhibits lipopolysaccharide-induced inflammation and stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via regulation of AKT/mTOR signaling

“…6 Moreover, lipopolysaccharides also impair osteoblast differentiation and suppress osteogenic differentiation tendency of BMSCs. 7 Suppression of lipopolysaccharide-stimulated inflammation and differentiation in BMSCs contributed to the amelioration of immuno-inflammatory oral diseases. 8 Galangin is a natural flavonoid extracted from Alpinia officinarum with pharmacological activities, such as anticancer, antibacterial, anti-inflammatory, hepatoprotective, and antiarthritic effects.…”