Berberine enhances posttranslational protein stability of p21/cip1 in breast cancer cells via down-regulation of Akt

Tak, Jyoti; Sabarwal, Akash; Shyanti, Ritis K.; Singh, Rana P.

doi:10.1007/s11010-019-03529-4

Cited by 29 publications

(17 citation statements)

References 32 publications

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“…An immobilized capture antibody specifically binds human p21. After washing away unbound material, a biotinylated detection antibody specific for human p21 is used to detect the captured protein, utilizing a standard Streptavidin-HRP format [ 55 ]. All experiments were performed in triplicates and absorbance was measured at λ = 450 nm using a Dynex plate reader.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin

et al. 2020

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Natural compounds can modulate all three major phases of carcinogenesis. The role of the natural compounds such as resveratrol (RSV) and curcumin (CRM) in modulation of anticancer potential of platinum-based drugs (CisPt) is still a topic of considerable debate. In order to enhance head and neck cancer (HNSCC) cells’ sensitivity to the cytotoxic effects of CisPt combined treatments with RSV or CRM were used. The study aim was to evaluate how the RSV or CRM associated to CisPt treatment modulated some cellular processes such as proliferation, P21 gene expression, apoptotic process, and cell cycle development in HNSCC tumor cell line (PE/CA-PJ49) compared to a normal cell line (HUVEC). The results showed that RSV or CRM treatment affected the viability of tumor cells more than normal cells. These natural compounds act against proliferation and sustain the effects of cisplatin by cell cycle arrest, induction of apoptosis and amplification of P21 expression in tumor cells. In conclusion, using RSV or CRM as adjuvants in CisPt therapy might have a beneficial effect by supporting the effects induced by CisPt.

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Section: Methodsmentioning

confidence: 99%

The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin

et al. 2020

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“…As with the case for various disease models, modulation of Akt has been shown to be the primary target of berberine in cancer cells. Hence, inhibition of Akt activation in gastric [39] and breast [25,45,51] cancer cells were observed. The other main targets or berberine in cancer are associated with metastasis.…”

Section: Induction Of Apoptosismentioning

confidence: 96%

“…The cell cycle arrest that vary depending on cell-type is initiated by targeting key checkpoint kinase targets leading to a reduction of, for example, cyclin D1 and cyclin E for G1 phase arrest [30] or cyclin D1, cyclin E, Cdk2, and Cdk4 for G0/G1 phase arrest [59]. In this regard, upregulation of cyclin-dependent kinase inhibitors (P21 and p27) by increasing their posttranslational stability through inhibition of their degradation is a further mechanism of action for berberine [25,50,52,53]. Table 1.…”

Section: The Anticancer Effect Of Berberine: Direct Cytotoxicity In Cmentioning

confidence: 99%

“…Induction of cell death. Tak et al [25] HeLa Reduce cell viability and induce apoptosis; suppress tumour growth in vivo; reduce cytosolic PLA2 and COX-2 protein levels; elevate the content ratio of arachidonic acid to PGE2.…”

Section: Cell Type or Animal Modelmentioning

confidence: 99%

“…Molecules 2020, 25 Wang et al [128] prepared a series of 3-O-and 9-O-derivatives of berberine with the hope to find a better cancer immunotherapeutic agent via targeting indoleamine 2,3-dioxygenase 1 (IDO1). This was based on the known overexpression level of IDO1 in cancer cells and modulatory role in cancer development and malignancies.…”

Section: The Berberine-o-derivativesmentioning

confidence: 99%

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Recent Advances in Berberine Inspired Anticancer Approaches: From Drug Combination to Novel Formulation Technology and Derivatization

Habtemariam

2020

Molecules

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Berberine is multifunctional natural product with potential to treat diverse pathological conditions. Its broad-spectrum anticancer effect through direct effect on cancer cell growth and metastasis have been established both in vitro and in vivo. The cellular targets that account to the anticancer effect of berberine are incredibly large and range from kinases (protein kinase B (Akt), mitogen activated protein kinases (MAPKs), cell cycle checkpoint kinases, etc.) and transcription factors to genes and protein regulators of cell survival, motility and death. The direct effect of berberine in cancer cells is however relatively weak and occur at moderate concentration range (10–100 µM) in most cancer cells. The poor pharmacokinetics profile resulting from poor absorption, efflux by permeability-glycoprotein (P-gc) and extensive metabolism in intestinal and hepatic cells are other dimensions of berberine’s limitation as anticancer agent. This communication addresses the research efforts during the last two decades that were devoted to enhancing the anticancer potential of berberine. Strategies highlighted include using berberine in combination with other chemotherapeutic agents either to reduce toxic side effects or enhance their anticancer effects; the various novel formulation approaches which by order of magnitude improved the pharmacokinetics of berberine; and semisynthetic approaches that enhanced potency by up to 100-fold.

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Anti-tumor effect of berberine on chronic lymphocytic leukemia cells

et al. 2022

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Berberine enhances posttranslational protein stability of p21/cip1 in breast cancer cells via down-regulation of Akt

Cited by 29 publications

References 32 publications

The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin

The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin

Recent Advances in Berberine Inspired Anticancer Approaches: From Drug Combination to Novel Formulation Technology and Derivatization

Anti-tumor effect of berberine on chronic lymphocytic leukemia cells

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