Berberine Enhances the Antibacterial Activity of Selected Antibiotics against Coagulase-Negative Staphylococcus Strains in Vitro

Abstract

Synergistic interactions between commonly used antibiotics and natural bioactive compounds may exhibit therapeutic benefits in a clinical setting. Berberine, an isoquinoline-type alkaloid isolated from many kinds of medicinal plants, has proven efficacy against a broad spectrum of microorganisms. The aim of the presented work was to assess the antibacterial activity of berberine chloride in light of the effect exerted by common antibiotics on fourteen reference strains of Staphylococccus spp., and to evaluate the magnitude of interactions of berberine with these antistaphylococcal antibiotics. In our study minimum inhibitory concentrations (MIC) of berberine chloride against CoNS ranged from 16 to 512 µg/mL. The most noticeable effects were observed for S. haemolyticus ATCC 29970, S. epidermidis ATCC 12228, S. capitis subsp. capitis ATCC 35661, S. galinarium ATCC 700401, S. hominis subsp. hominis ATCC 27844, S. intermedius ATCC 29663 and S. lugdunensis ATCC 49576. The most significant synergistic effect was noticed for berberine in combination with linezolid, cefoxitin and erythromycin. The

Berberis Vulgaris and Berberine: An Update Review

“…A Recent study indicates that the primary mechanism of antibacterial action of berberine is due to inhibition of the cell division protein FtsZ (Boberek et al ., ). Berberine has a synergistic effect with some common antibiotics especially with linezolid, cefoxitin, and erythromycin (Wojtyczka et al ., ). This suggests the potential use of berberine in combinations with other antibiotics as an efficient therapeutic tool for antibiotic‐resistant bacterial infections.…”

“…Berberine is widely used as an anti‐infective in traditional medicine (Boberek et al ., ). Berberine has been shown to exhibit antibacterial activity against a variety of bacteria, including Streptococcus agalactiae (MIC = 78 mug/mL) (Peng et al ., ), Actinobacillus pleuropneumoniae (MIC = 0.3125 mg/mL) (Kang et al ., ), different strains of Staphylococccus (MIC = from 16 to 512 micro g/mL) (Wojtyczka et al ., ) and, Shigella dysenteriae (Kong et al , ). Berberine also effectively prevents the formation of S .…”

Antioxidant Effect of Berberine and its Phenolic Derivatives Against Human Fibrosarcoma Cells

“…Berberine, an isoquinoline alkaloid, has been used for traditional medical systems in eastern countries. It possesses several biological activities including anti-inflammatory (Chu et al, 2014), anticancer (Yip and Ho, 2013;Cai et al, 2014), anti-diabetic (Rajendran et al, 2014), antibacterial (Wojtyczka et al, 2014), and antifungal functions (Zhu et al, 2014). The antioxidant capacity of berberine has demonstrated both direct (Jang et al, 2009;Bashir and Gilani, 2011) and indirect activities (Hwang et al, 2002;Tan et al, 2007).…”

Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing: in vitro and in vivo studies

“…Kang et al [48] reported that berberine exhibited time and concentration dependence antibacterial activity against Actinobacillus pleuropneumoniae with the MIC of 0.3125 mg/mL. Wojtyczka et al [50] reported that berberine has antibacterial activity against coagulase-negative staphylococcus strains with the MIC ranged from 16 to 512 μg/mL. The higher bactericidal effect of CA/Gel/Beri dressing observed in our study is related to the incorporation berberine in the matrix of CA/Gel nanofibers, while this value is effective for wound dressing applications.…”