Berberine Exerts Neuroprotective Actions against in Vitro Ischemia-Induced Neuronal Cell Damage in Organotypic Hippocampal Slice Cultures: Involvement of B-Cell Lymphoma 2 Phosphorylation Suppression

Cui, Hu-Shan; Matsumoto, Kinzo; Murakami, Yukihisa; Hori, Hitomi; Zhao, Qi; Obi, Ryosuke

doi:10.1248/bpb.32.79

Cited by 66 publications

(41 citation statements)

References 46 publications

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“…Western blotting was performed as previously described (Cui et al, 2009;Zhao et al, 2010). Briefly, after completing the behavioral experiments, the animals were decapitated and the hippocampal tissues were dissected.…”

Section: Western Blottingmentioning

confidence: 99%

Ameliorative effects of yokukansan on learning and memory deficits in olfactory bulbectomized mice

Yamada

Hayashida

Zhao

et al. 2011

Journal of Ethnopharmacology

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Section: Western Blottingmentioning

confidence: 99%

Ameliorative effects of yokukansan on learning and memory deficits in olfactory bulbectomized mice

Yamada

Hayashida

Zhao

et al. 2011

Journal of Ethnopharmacology

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“…Western blotting was performed by a slightly modified version of a method described previously (5,16,21). Briefly, tissue samples were taken from the hippocampus and proteins were extracted by homogenization in protein lysis buffer [50 mM Tris (pH 7.4), 150 mM NaCl, 0.5% sodium deoxycholate, 1% (v/v) NP-40, 0.1% (v/v) sodium dodecyl sulfate (SDS), 150 mM NaF, 8.12 μg/ml aprotinin, 2 mM sodium orthovanadate, 10 μg/ml leupeptin, and 2 mM phenylmethylsulfonyl fluoride] using TissueLyser ® (Qiagen, Osaka).…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Determination of cholinesterase activity was performed on the basis of the colorimetric method as previously described (13,21). Briefly, the frozen cortex was weighed and homogenized in 10 volumes of 0.1 M phosphate buffer (pH 7.4) containing 1% Triton-X-100.…”

Section: Ex Vivo Measurement Of Cholinesterase Activity In the Brainmentioning

confidence: 99%

Diabetes-Induced Central Cholinergic Neuronal Loss and Cognitive Deficit Are Attenuated by Tacrine and a Chinese Herbal Prescription, Kangen-Karyu: Elucidation in Type 2 Diabetes db/db Mice

et al. 2011

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Abstract.We investigated the effect of kangen-karyu (KK), a Chinese herbal prescription, on cognitive deficits and central cholinergic systems of type 2 diabetic db/db mice. Seven-week-old db/db (Y-db/db) mice received daily administration of test drugs during an experimental period of 12 weeks. At 18 weeks of age (O-db/db), the animals underwent the water maze test. Compared with age-matched control strain mice (O-m/m), vehicle-treated O-db/db mice showed impaired learning and memory performance. KK (100 -200 mg/kg per day) and the reference drug tacrine (THA: 2.5 mg/kg per day) ameliorated the performance of O-db/db mice without affecting their serum glucose level. O-db/db mice had lower levels of brain-derived neurotrophic factor (BDNF) mRNA and its protein in the brain than O-m/m mice. Expression levels of central cholinergic marker proteins in the hippocampus and the number of cholinergic cells in the medial septum and basal forebrain were also significantly lower in O-db/db than in O-m/m mice, whereas no significant differences in the expression levels of these factors and the cell number were found between Y-m/m and Y-db/db mice. KK and THA treatment significantly reversed the down-regulated levels of cholinergic markers, choline acetyltransferase-positive cell number, and BDNF expression in db/db mice. These findings suggest that KK as well as THA prevents diabetes-induced cognitive deficits by attenuating dysfunction of central cholinergic systems.

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“…Western blotting was performed by a slightly modified method described previously (Cuia et al, 2009). Briefly, tissue samples were taken from the cortex and proteins were extracted by homogenization in protein lysis buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 0.5% sodium deoxycholate, 1% (v/v) NP-40, 0.1% (v/v) sodium dodecyl sulfate (SDS), 150 mM NaF, 8.12 g/ml aprotinin, 2 mM sodium orthovanadate, 10 g/ml leupeptin, and 2 mM phenylmethlsulfonyl fluoride using TissueLyser ® (Qiagen, Osaka, Japan).…”

Section: Western Blot Analysesmentioning

confidence: 99%

Kangen-karyu improves memory deficit caused by aging through normalization of neuro-plasticity-related signaling system and VEGF system in the brain

Zhao

Yokozawa

Yamabe

et al. 2010

Journal of Ethnopharmacology

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Berberine Exerts Neuroprotective Actions against in Vitro Ischemia-Induced Neuronal Cell Damage in Organotypic Hippocampal Slice Cultures: Involvement of B-Cell Lymphoma 2 Phosphorylation Suppression

Cited by 66 publications

References 46 publications

Ameliorative effects of yokukansan on learning and memory deficits in olfactory bulbectomized mice

Ameliorative effects of yokukansan on learning and memory deficits in olfactory bulbectomized mice

Diabetes-Induced Central Cholinergic Neuronal Loss and Cognitive Deficit Are Attenuated by Tacrine and a Chinese Herbal Prescription, Kangen-Karyu: Elucidation in Type 2 Diabetes db/db Mice

Kangen-karyu improves memory deficit caused by aging through normalization of neuro-plasticity-related signaling system and VEGF system in the brain

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