Berberine improved intestinal barrier function by modulating the intestinal microbiota in blunt snout bream (Megalobrama amblycephala) under dietary high-fat and high-carbohydrate stress

Yu, Chengbing; Zhang, Jing; Qin, Qin; Jin, Liu; Xu, Jianxiong; Xu, Weina

doi:10.1016/j.fsi.2020.04.052

Cited by 50 publications

(46 citation statements)

References 59 publications

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“…In the present study, the HC diets significantly affected growth, immunity, carbohydrate metabolism, lipid metabolism, and the health of liver tissue, which are similar to those observed in the blunt snout bream (Megalobrama amblycephala), Nile tilapia (Oreochromis niloticus) and European seabass (Dicentrarchus labrax) fed HC diets [5,23,24]. In the present study, the HC feed increased the weight gain and HSI, but dietary MI supplementation decreased HSI.…”

Section: Discussionsupporting

confidence: 83%

Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus)

Zhu

Pan

Wang

et al. 2020

Animals

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This study investigated the effect of dietary myo-inositol (MI) on alleviating the adverse effect of the high carbohydrate diet in Nile tilapia (Oreochromis niloticus). Six diets contained either low carbohydrate (LC 30%) or high carbohydrate (HC 45%) with three levels of MI supplementation (0, 400 and 1200 mg/kg diet) to each level of the carbohydrate diet. After an 8-week trial, the fish fed 400 mg/kg MI under HC levels had the highest weight gain and fatness, but the fish fed 1200 mg/kg MI had the lowest hepatosomatic index, visceral index and crude lipid in the HC group. The diet of 1200 mg/kg MI significantly decreased triglyceride content in the serum and liver compared with those fed the MI supplemented diets regardless of carbohydrate levels. Dietary MI decreased triglyceride accumulation in the liver irrespective of carbohydrate levels. The content of malondialdehyde decreased with increasing dietary MI at both carbohydrate levels. Fish fed 1200 mg/kg MI had the highest glutathione peroxidase, superoxide dismutase, aspartate aminotransferase and glutamic-pyruvic transaminase activities. The HC diet increased the mRNA expression of key genes involved in lipid synthesis (DGAT, SREBP, FAS) in the fish fed the diet without MI supplementation. Dietary MI significantly under expressed fatty acid synthetase in fish fed the HC diets. Moreover, the mRNA expression of genes related to lipid catabolism (CPT, ATGL, PPAR-α) was significantly up-regulated with the increase of dietary MI levels despite dietary carbohydrate levels. The gene expressions of gluconeogenesis, glycolysis and MI biosynthesis were significantly down-regulated, while the expression of the pentose phosphate pathway was up-regulated with the increase of MI levels. This study indicates that HC diets can interrupt normal lipid metabolism and tend to form a fatty liver in fish. Dietary MI supplement can alleviate lipid accumulation in the liver by diverging some glucose metabolism into the pentose phosphate pathway and enhance the antioxidant capacity in O. niloticus.

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Section: Discussionsupporting

confidence: 83%

Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus)

Zhu

Pan

Wang

et al. 2020

Animals

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“…The present results showed that diet EHL could reduce the expression of claudin14, claudin18 and JAM-A in intestine of tiger puffer, indicating that high-lipid diets can destroy intestinal TJ structure, increase intestinal permeability and make intestine more vulnerable to pathogens, which is further confirmed by the increased activity of serum DAO. Studies in Nile tilapia, zebrafish and blunt snout bream had also found that a high-lipid diet can destroy the physical barrier of intestine (Arias-Jayo et al, 2018;Yu et al, 2020;. During lipid absorption, intestinal epithelial cells are temporarily damaged and need about 50 min to repair, while a continuous feeding of high-lipid diets can lead to the accumulation of chylomicron in the intestine.…”

Section: The Effects Of High-lipid Diet On the Intestinal Physical Barriermentioning

confidence: 99%

Effects of different dietary lipid levels on intestinal mucosal barrier and microbial community of juvenile tiger puffer Takifugu rubripes

Kong

Liao

et al. 2021

Aquacult Nutr

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“…It is well known that the intestine is an important physical barrier for sh, and the structural integrity is crucial for resistance to foreign antigens (He et al, 2013;Zhao et al, 2020).This study found that 17% starch diet impaired the intestinal mucosa and increased the number of in ammatory cells. Similar results were found in blunt snout bream (Megalobrama amblycephala) (Yu et al, 2020). Moreover, high-fat diet damage the intestinal barrier in zebra sh, carbohydrate and fat are important for energy supply, and when the energy exceeded the body's needs, stress occurred, resulting in intestinal impairment (Arias-Jayo et al, 2018).The physical barrier of animal intestine is not only closely related to the complete cellular structure but also the epithelial permeability (Bergmann et al, 2013;Liu et al, 2014).…”

Section: High-starch Diet Impaired Intestinal Physical Barriermentioning

confidence: 68%

“…In present study, the activity of LZM conspicuously increased in H group, 17% starch diet up-regulated the mRNA levels of hepcidin1 TNF-α IL-8 and IL-1βin the intestine, which indicated high starch diet could induce intestinal in ammation. Similarly, high starch diet up-regulated intestinal in ammation-related mRNA levels in Nile tilapia, turbot and blunt snout bream (Bai et al, 2019;Yu et al, 2020). It was observed that 17% starch diet impaired the intestinal epithelium and increased intestinal permeability, making it easier for harmful substances during food digestion and pathogens produced to enter the intestinal tissues, and ultimately resulted intestinal in ammation.…”

Section: High-starch Diet Impaired Intestinal Physiological Barriermentioning

confidence: 99%

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High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

Zhao¹,

Ji²,

Liao³

et al. 2021

Preprint

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The intestinal barrier is primarily composed of physical, physiological and microbial barriers, and intestine microbe-immune interactions influenced by diet in fish still remain increasingly unexplored. The intestinal barriers of Largemouth bass (Micropterus salmoides) (4.1±0.2g) were explored after they were fed three different starch diets for eight weeks (low starch,7%; middle starch, 12%; high starch, 17%). The results showed that high starch diet led slight infiltration of inflammatory cells and moderate loss of mucous membrane layer in midgut. Meanwhile, high starch diet decreased the antioxidant enzymes (T-SOD) activities and genes (SOD1, SOD2, SOD3a, CAT, GPX) expression significantly (P < 0.05). The MDA content was significantly increased with increasing starch level (P < 0.05). High starch diet up-regulated the expression of pro-inflammatory genes (IL-8, IL-1β and TNFα) and apoptosis genes (bax, caspase3, caspase8 and caspase9), whereas down-regulated the expression of anti-apoptosis genes bcl-2 and tight junction proteins genes (occludin and claudin7). In addition, the high starch diet increased the relative abundance of Actinobacteria and Firmicutes, resulted in microbial dysbiosis. In conclusion, high dietary starch impaired intestinal barriers, and increased the risk of disease outbreaks.

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Berberine improved intestinal barrier function by modulating the intestinal microbiota in blunt snout bream (Megalobrama amblycephala) under dietary high-fat and high-carbohydrate stress

Cited by 50 publications

References 59 publications

Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus)

Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus)

Effects of different dietary lipid levels on intestinal mucosal barrier and microbial community of juvenile tiger puffer Takifugu rubripes

High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

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