Berberine Inhibited the Growth of Thyroid Cancer Cell Lines 8505C and TPC1

Park, Kyoung Sik; Kim, Jong Bin; Bae, Jaeman; Park, Seo-Young; Jee, Hyeon-Gun; Lee, Kyu Eun; Youn, Yeo-Kyu

doi:10.3349/ymj.2012.53.2.346

Cited by 38 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with our results, it had been reported that berberine blocks injury‐induced vascular smooth muscle cells proliferation by attenuation of MEK/ERK activation . In addition, a recent report indicated that berberine induces G0/G1 cell cycle arrest in TPC1 cells by accumulation of p27 protein . Together with these reports, it was suggested that berberine induces G1 cell cycle arrest in Bel7402 cells partially via disturbing the interaction of CaM with CaMKII and blocking subsequent p27 protein degradation.…”

Section: Discussionsupporting

confidence: 92%

Calmodulin as a Potential Target by Which Berberine Induces Cell Cycle Arrest in Human Hepatoma Bel7402 Cells

et al. 2013

View full text Add to dashboard Cite

Berberine is an isoquinoline alkaloid that has drawn extensive attention because it possesses various biological activities. Several mechanisms have been proposed to interpret the anticancer activity of berberine. However, these explanations are mostly based on its downstream-regulated genes or proteins; information on the direct target proteins that mediate the antiproliferative action of berberine remains unclear. In this study, a computational pipeline based on a ligand-protein inverse docking program and mining of the 'Connectivity MAP' data was adopted to explore the potential target proteins for berberine. The results showed that four proteins, that is calmodulin, cytochrome P450 3A4, sex hormone-binding globulin, and carbonic anhydrase II, were suggested to be the potential targets of berberine. The anticalmodulin property of berberine was demonstrated with an in vitro phosphodiesterase activity assay. Flow cytometric analysis found that G1 cell cycle arrest induced by berberine in Bel7402 cells was enhanced by cotreatment with calmodulin inhibitors. Western blotting results indicated that berberine treatment decreased phosphorylation of calmodulin kinase II and blocked subsequent MEK1 activation as well as p27 protein degradation. These results suggested that calmodulin might play crucial roles in berberine-induced cell cycle arrest in cancer cells.

show abstract

Section: Discussionsupporting

confidence: 92%

Calmodulin as a Potential Target by Which Berberine Induces Cell Cycle Arrest in Human Hepatoma Bel7402 Cells

et al. 2013

View full text Add to dashboard Cite

show abstract

“…It has been shown that berberine blocks Wnt/β-catenin signalling, inhibits cell migration (Park et al, 2012;Albring et al, 2013) and has the ability to regulate cell attachment through zonula occludens 1 protein (ZO-1) (Liu et al, 2009). Berberine can activate ZO-1, helps in the formation of tight junctions between cells and reduces cell mobility.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the anti-cancer potential of Mahonia aquifolium extracts via apoptosis and anti-angiogenesis

Damjanović

Zdunić²,

Šavikin³

et al. 2016

Bangladesh J Pharmacol

View full text Add to dashboard Cite

The cytotoxicity of Mahonia aquifolium ethanol and water extracts was examined using MTT test. The morphological changes were analyzed by fluorescence microscopy. Cell cycle distribution and possible activation of caspase-dependent pathway of cell death were assessed by flow cytometry. The effects of ethanol and water extracts on migration of endothelial EA.hy926 cells were analyzed by in vitro scratch assay and inhibition of angiogenesis was detected using tube formation assay. Both extracts demonstrated cytotoxic effects on cancer cell lines with very high selectivity. Morphological evaluation indicated apoptosis. These results were confirmed with cell cycle analysis, where there was accumulation of cancer cells in the subG1 phase. Ethanol and water extracts induced a caspase-dependent apoptosis in HeLa cells through activation of caspase-3 and caspase-8. Both extracts showed the ability to inbibit the migration of EA.hy926 cells and initial steps of angiogenesis. In addition, ethanol extract exerted significant anti-angiogenic effect.Video Clip:<a href="https://www.youtube.com/v/_b1LobagxyA">Cell seeding</a>: 2 min 26 sec<a href="https://www.youtube.com/v/LZrwdyvFAyI">4 hours after treatment of MTT-SDS applied</a>: 54 sec<a href="https://www.youtube.com/v/a6Dshdsg8TY">72 hours after treatment MTT applied</a>: 1 min 8 sec<a href="https://www.youtube.com/v/RE_SH-CauRU">Day 2 treatment</a>: 1 min 32 sec<a href="https://www.youtube.com/v/l4E1y0lgrAE">Measuring absorbance</a>: 9 sec

show abstract

“…The cell cycle arrest that vary depending on cell-type is initiated by targeting key checkpoint kinase targets leading to a reduction of, for example, cyclin D1 and cyclin E for G1 phase arrest [30] or cyclin D1, cyclin E, Cdk2, and Cdk4 for G0/G1 phase arrest [59]. In this regard, upregulation of cyclin-dependent kinase inhibitors (P21 and p27) by increasing their posttranslational stability through inhibition of their degradation is a further mechanism of action for berberine [25,50,52,53]. Table 1.…”

Section: The Anticancer Effect Of Berberine: Direct Cytotoxicity In Cmentioning

confidence: 99%

Recent Advances in Berberine Inspired Anticancer Approaches: From Drug Combination to Novel Formulation Technology and Derivatization

Habtemariam

2020

Molecules

View full text Add to dashboard Cite

Berberine is multifunctional natural product with potential to treat diverse pathological conditions. Its broad-spectrum anticancer effect through direct effect on cancer cell growth and metastasis have been established both in vitro and in vivo. The cellular targets that account to the anticancer effect of berberine are incredibly large and range from kinases (protein kinase B (Akt), mitogen activated protein kinases (MAPKs), cell cycle checkpoint kinases, etc.) and transcription factors to genes and protein regulators of cell survival, motility and death. The direct effect of berberine in cancer cells is however relatively weak and occur at moderate concentration range (10–100 µM) in most cancer cells. The poor pharmacokinetics profile resulting from poor absorption, efflux by permeability-glycoprotein (P-gc) and extensive metabolism in intestinal and hepatic cells are other dimensions of berberine’s limitation as anticancer agent. This communication addresses the research efforts during the last two decades that were devoted to enhancing the anticancer potential of berberine. Strategies highlighted include using berberine in combination with other chemotherapeutic agents either to reduce toxic side effects or enhance their anticancer effects; the various novel formulation approaches which by order of magnitude improved the pharmacokinetics of berberine; and semisynthetic approaches that enhanced potency by up to 100-fold.

show abstract

Berberine Inhibited the Growth of Thyroid Cancer Cell Lines 8505C and TPC1

Cited by 38 publications

References 20 publications

Calmodulin as a Potential Target by Which Berberine Induces Cell Cycle Arrest in Human Hepatoma Bel7402 Cells

Calmodulin as a Potential Target by Which Berberine Induces Cell Cycle Arrest in Human Hepatoma Bel7402 Cells

Evaluation of the anti-cancer potential of <i>Mahonia aquifolium</i> extracts via apoptosis and anti-angiogenesis

Recent Advances in Berberine Inspired Anticancer Approaches: From Drug Combination to Novel Formulation Technology and Derivatization

Contact Info

Product

Resources

About