Berberine suppresses amyloid-beta-induced inflammatory response in microglia by inhibiting nuclear factor-kappaB and mitogen-activated protein kinase signalling pathways

Jia, Ling; Liu, Jing; Song, Zhen; Pan, Xiaohua; Chen, Liang; Cui, Xing; Wang, Molin

doi:10.1111/j.2042-7158.2012.01529.x

Cited by 115 publications

(72 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TNF-α is also known as cachectin, due to the fact that it mediates fever and cachexia, and is responsible for the numerous detrimental effects associated with bacterial sepsis, rheumatoid arthritis and Crohn's disease. TNF-α is released by monocytes and macrophages in response to various stimuli including bacterial LPS, which is a principal mediator of the deleterious effects of endotoxin (31). IL-1 is an inflammatory cytokine that is released in response to infection or cell injury by cells of the innate immune system, such as macrophages.…”

Section: Discussionmentioning

confidence: 99%

Berberine protects against esophageal mucosal damage in reflux esophagitis by suppressing proinflammatory cytokines

Choo

Roh

2013

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

This study was performed to investigate the effects of berberine (BB) in a rat model of gastroesophageal reflux disease (GERD), induced by pylorus and forestomach ligation. We evaluated cytotoxicity and proinflammatory biomarkers (nitric oxide, interleukin (IL)-1β and prostaglandin E2) in RAW 264.7 cells in vitro and anti-inflammatory effects in vivo. A total of 54 Sprague Dawley rats were divided into six groups: intact control rats; reflux esophagitis (RE) control rats; RE rats treated with 20 mg/kg omeprazole and RE rats treated with BB at doses of 20, 40 and 60 mg/kg, respectively. All rats were fasted. RE was induced by pylorus and forestomach ligation one hour subsequent to the oral treatment. Six hours subsequent to the surgery, the rats were sacrificed, blood was collected from the abdominal vein and the esophagus and stomach were dissected. The gastric volume and the pH of the gastric juice were evaluated, prior to the esophagus being cut longitudinally and an inner mucosal area being imaged, to analyze mucosal damage indices. Proinflammatory biomarkers in the serum, including tumor necrosis factor (TNF)-α, IL-1β, IL-6 and monocyte chemoattractant protein (MCP)-1 were analyzed using an enzyme-linked immunosorbent assay (ELISA) kit, while the mRNA expression of TNF-α, IL-1β, IL-6 and plasminogen activator inhibitor (PAI)-1 was analyzed using a quantitative polymerase chain reaction (qPCR). Esophagic tissue damage in the BB groups was dose-dependently decreased compared with that in the RE control group. This result was consistent with significant reductions in the levels of proinflammatory biomarkers in the serum and in the expression of proinflammatory mRNA, specifically, TNF-α, IL-1β, IL-6 and PAI-1. The results suggest that the anti-inflammatory and protective effects of BB may attenuate the severity of RE and prevent esophageal mucosal damage, in addition to validating the use of BB as a pharmacological treatment for esophageal reflux disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Berberine protects against esophageal mucosal damage in reflux esophagitis by suppressing proinflammatory cytokines

Choo

Roh

2013

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…44,45 Considerable evidence has accumulated to emphasize the importance of inflammatory processes in the etiology of diabetic complications, including diabetic neuropathy. 46,47 The NF-jB inflammatory cascade occupies an intermediate position in the schema of pathogenesis, downstream of oxidative stress, advanced glycation end-product formation, and mitogen-activated protein kinase activation, but also contributing to them.…”

mentioning

confidence: 99%

Berberine Ameliorates Cold and Mechanical Allodynia in a Rat Model of Diabetic Neuropathy

Kim

2013

Journal of Medicinal Food

View full text Add to dashboard Cite

This study evaluated the antiallodynic properties of berberine on cold and mechanical allodynia after streptozotocin (STZ)-induced diabetes using a rat model. Diabetic neuropathy was induced in rats by intraperitoneal injection of STZ. To measure cold and mechanical allodynia, a 4°C plate and von Frey filament were used, respectively. Cold and mechanical allodynia induced by diabetes were significantly decreased by single and repeated intraperitoneal treatment of amitriptyline at 10 mg/kg, and berberine at 10 and 20 mg/kg. The hepatic malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase activities were significantly increased in diabetic rats as compared with those in intact rats; however, in amitriptyline-and berberine-treated rats, they were significantly decreased as compared to the STZ control. The overall effects of berberine 20 mg/kg on cold and mechanical allodynia were quite similar to those of amitriptyline 10 mg/kg, and berberine exhibited similar antioxidant effects as the same dosage of amitriptyline. In conclusion, berberine (10 and 20 mg/kg) was observed to have antiallodynic effects against diabetes, which are presumed to be associated with antioxidative effects. It can be considered that the anti-inflammatory or antidepressant capacity of berberine could contribute to the antiallonynic effects shown in this study.

show abstract

“…By blocking the ubiquitin proteasome system (UPS) and the autophagy-lysosomal pathway, BBR inhibited HNF1α-mediated PCSK9 transcription via UPS to decrease the hepatic HNF1α protein levels without affecting the mRNA levels [86] . Several studies have also reported that BBR has neuroprotective effects (such as suppressed neuronal apoptosis [88] and neuroinflammation [89] ) and both anti-apoptotic and antiinflammatory effects [90,91] . Additional studies are needed to determine whether BBR exerts its effects through PCSK9.…”

Section: Berberine (Bbr)mentioning

confidence: 99%

Lowering serum lipids via PCSK9-targeting drugs: current advances and future perspectives

et al. 2017

Acta Pharmacol Sin

View full text Add to dashboard Cite

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as neural apoptosis regulated convertase (NARC1), is a key modulator of cholesterol metabolism. PCSK9 increases the serum concentration of low-density lipoprotein cholesterol by escorting low-density lipoprotein receptors (LDLRs) from the membrane of hepatic cells into lysosomes, where the LDLRs are degraded. Owing to the importance of PCSK9 in lipid metabolism, considerable effort has been made over the past decade in developing drugs targeting PCSK9 to lower serum lipid levels. Nevertheless, some problems and challenges remain. In this review we first describes the structure and function of PCSK9 and its gene polymorphisms. We then discuss the various designs of pharmacological targets of PCSK9, including those that block the binding of PCSK9 to hepatic LDLRs (mimetic peptides, adnectins, and monoclonal antibodies), inhibit PCSK9 expression (the clustered regularly interspaced short palindromic repeats/Cas9 platform, small molecules, antisense oligonucleotides, and small interfering RNAs), and interfere with PCSK9 secretion. Finally, this review highlights future challenges in this field, including safety concerns associated with PCSK9 monoclonal antibodies, the limited utility of PCSK9 inhibitors in the central nervous system, and the cost-effectiveness of PCSK9 inhibitors.

show abstract

Berberine suppresses amyloid-beta-induced inflammatory response in microglia by inhibiting nuclear factor-kappaB and mitogen-activated protein kinase signalling pathways

Abstract: Our data indicated berberine is a potent suppressor of neuroflammation, presumably through inhibition of NF-κB activation, and suggested berberine has therapeutic potential for the treatment of neuroinflammation that is involved in neurological diseases such as AD.

Cited by 115 publications

References 55 publications

Berberine protects against esophageal mucosal damage in reflux esophagitis by suppressing proinflammatory cytokines

Berberine protects against esophageal mucosal damage in reflux esophagitis by suppressing proinflammatory cytokines

Berberine Ameliorates Cold and Mechanical Allodynia in a Rat Model of Diabetic Neuropathy

Lowering serum lipids via PCSK9-targeting drugs: current advances and future perspectives

Contact Info

Product

Resources

About