Berberis kansuensis extract alleviates type 2 diabetes in rats by regulating gut microbiota composition

Xu, Tong; Ge, Yiman; Du, Huan; Li, Qi; Xu, Xinmei; Huan, Yi; Wu, Xinyue; Kuang, Tingting; Fan, Gang

doi:10.1016/j.jep.2021.113995

Cited by 46 publications

(31 citation statements)

References 26 publications

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“…Our results also showed positive correlations between Escherichia-Shigella, weight loss and IL-6. The results are in line with those by Rubio et al who concluded that Escherichia-Shigella negatively correlated with weight gain and final weight of broilers (50); Escherichia-Shigella were positively correlated with IL-6 in rats with type 2 diabetes (51). Therefore, the decreased abundance of Escherichia-Shigella following LA pretreatment indicated the protective effects of LA against LPS exposure.…”

Section: Discussionsupporting

confidence: 91%

Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers

Wu¹,

Li²,

Liu³

et al. 2021

Front. Immunol.

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Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals. This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge. A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA. After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline. Then, the birds were sacrificed. Results showed that LA pretreatment significantly alleviated the weight loss and intestinal mucosal injuries caused by LPS challenge. LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10. Metabolomics analysis revealed a significant difference of serum metabolites by LA pretreatment. Twenty-seven serum metabolic biomarkers were identified and mostly belong to lipids. LA also markedly modulated the pathway for sphingolipid metabolism, suggesting its ability to regulate lipid metabolism. Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs). Pearson’s correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition. The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.

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Section: Discussionsupporting

confidence: 91%

Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers

Wu¹,

Li²,

Liu³

et al. 2021

Front. Immunol.

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“…The relationship between Faecalibacterium and metabolism has been veri ed in mouse model [23,24]. In addition, the application of traditional Chinese medicine extract can remarkably reduce the abundance of Fusobacterium in type 2 diabetic rats [25]. Our results are in line with the current research, and it can be used as a supplement to the related research on gut microbiota and IR.…”

Section: Discussionsupporting

confidence: 85%

Characteristics of Gut Microbiota and Its Relationship With Serum Sex Hormones in Non-obese Polycystic Ovary Syndrome Patients With Insulin Resistance

Cai¹,

Ni²,

Yao³

et al. 2022

Preprint

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Background: Insulin resistance (IR) is a common symptom in obese polycystic ovary syndrome (PCOS) patients, as well as in non-obese PCOS patients. Gut microbiota is closely related to IR. However, the relationship between gut microbiota and IR remains unclear in non-obese PCOS patients. Objective: To analyze the composition of the gut microbiota population in non-obese PCOS-IR patients compared to PCOS alone and healthy women. Methods: In the present study, fecal samples from non-obese PCOS patients with and without IR and healthy women were analyzed by 16S rRNA gene sequencing. Moreover, serum hormone levels were detected on the third day of menstruation, and the correlation between gut microbiota and serum hormone was analyzed using R language.Results: We found that the gut microbiota character differed in each group, and the gut microbiota abundance of PCOS-IR group was remarkably reduced.The abundance of Ruminococcaceae_UCG-004 and Acetanaerobacterium in the PCOS-IR group was significantly lower than that of the PCOS-NIR group. Furthermore, the comparison between PCOS-IR and control group revealed that g__Fusobacterium and g__Faecalibaculum were the unique genus of the PCOS-IR group. Moreover, the relationship between gut microbiota, IR, and serum sex hormones was determined. In PCOS with IR group, the relationship between gut microbiota and androgen was more significant. In PCOS without IR group, the relationship between gut microbiota and androgen was no significant. Its mechanism may be related to the different abundance in enzymes (futalosine hydrolase, arogenate dehydrogenase (NADP(+), and L-lysine 6-transaminase) and metabolic pathways (isoquinoline alkaloid biosynthesis, styrene degradation, and atrazine degradation), but it needs to be further explored in subsequent experiments. Conclusion: The gut microbiota composition of non-obese PCOS patients with insulin resistance is different from that of PCOS alone and healthy women. The difference is correlated with the clinical characteristics of PCOS, with regards to insulin resistance and sex hormone. The abnormality of gut microbiota may lead to the occurrence and development of PCOS-IR. All in all, this study not only provided supplements for the existing studies, but also revealed the potential pathogenic bacteria genus, thus providing a new idea for the treatment of PCOS with IR.

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“…However, since we did not set up a pure polysaccharide group, it is not clear whether this effect of promoting probiotic growth is synergistic. Fusobacterium positively correlated with fasting blood glucose, glycosylated serum protein, LPS, insulin resistance index (HOMA-IR), and three inflammatory factors (TNF-alpha, IL-1 beta, and IL-6) in rats [ 49 ]. We compared the abundance of Fusobacterium ( Figure 6 F) in our six groups after 24 h of in vitro fermentation.…”

Section: Resultsmentioning

confidence: 99%

Dietary Fiber Modulates the Fermentation Patterns of Cyanidin-3-O-Glucoside in a Fiber-Type Dependent Manner

Yang

Huang

et al. 2021

Foods

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The interactions between cell-wall polysaccharides and polyphenols in the gastrointestinal tract have attracted extensive attention. We hypothesized that dietary fiber modulates the fermentation patterns of cyanidin-3-O-glucoside (C3G) in a fiber-type-dependent manner. In the present study, the effects of four dietary fibers (fructose-oligosaccharides, pectin, β-glucan and arabinoxylan) on the modulation of C3G fermentation patterns were investigated through in vitro fermentation inoculated with human feces. The changes in gas volume, pH, total carbohydrate content, metabolites of C3G, antioxidant activity, and microbial community distribution during in vitro fermentation were analyzed. After 24 h of fermentation, the gas volume and total carbohydrate contents of the four dietary-fiber-supplemented groups respectively increased and decreased to varying degrees. The results showed that the C3G metabolites after in vitro fermentation mainly included cyanidin, protocatechuic acid, 2,4,6-trihydroxybenzoic acid, and 2,4,6-trihydroxybenzaldehyde. Supplementation of dietary fibers changed the proportions of C3G metabolites depending on the structures. Dietary fibers increased the production of short-chain fatty acids and the relative abundance of gut microbiota Bifidobacterium and Lactobacillus, thus potentially maintaining colonic health to a certain extent. In conclusion, the used dietary fibers modulate the fermentation patterns of C3G in a fiber-type-dependent manner.

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Berberis kansuensis extract alleviates type 2 diabetes in rats by regulating gut microbiota composition

Cited by 46 publications

References 26 publications

Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers

Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers

Characteristics of Gut Microbiota and Its Relationship With Serum Sex Hormones in Non-obese Polycystic Ovary Syndrome Patients With Insulin Resistance

Dietary Fiber Modulates the Fermentation Patterns of Cyanidin-3-O-Glucoside in a Fiber-Type Dependent Manner

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