In tropical regions, the viability of outdoor photo-fermentative biohydrogen production faces challenges arising from elevated temperatures and varying light intensity. This research aimed to explore how high temperatures and outdoor environments impact both biohydrogen production and the growth of purple non-sulfur bacteria. The ndings revealed the potential of Rhodopseudomonas spp. as a robust outdoor hydrogen-producing bacteria, demonstrating its capacity to thrive and generate biohydrogen even at 40°C and under uctuating outdoor conditions. Notably, Rhodopseudomonas pentothenatexigens KKU-SN1/1 exhibited the highest cumulative biohydrogen production of 400 mL/L under outdoor conditions. In addition, the outdoor enhancement of biohydrogen production was achieved through the utilization of a cluster of ten bioreactors system. The outcomes demonstrated a notable improvement in biohydrogen production e ciency, marked the highest daily biohydrogen production was 493 mL/L/day. Signi cantly, the highest biohydrogen production rate was noted to be 17 times greater than that observed in conventional batch production methods. This study is the rst report represents the inaugural utilization of R. pentothenatexigens for sustained biohydrogen production in the outdoor conditions, facilitated by the cluster of ten bioreactors system over an extended operational period.