Best of both: A simultaneous analysis of mRNA and miRNA markers for body fluid identification

Bamberg, Malte; Bruder, Maria; Dierig, Lisa; Kunz, Sebastian; Schmidt, Max; Wiegand, Peter

doi:10.1016/j.fsigen.2022.102707

Cited by 19 publications

(6 citation statements)

References 39 publications

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“…In our study, miR-484 had an unbalanced expression in six stains ( Figure 6 D), with a high expression level in the VB group. A similar trend was reported by Bamberg et al [ 65 ], except for the moderate expression observed in semen in this study. A conflict was observed in the miR-135b marker ( Figure 6 E), which was thought to be SA-specific [ 51 ] or SE-specific [ 48 , 50 , 55 ] in previous studies.…”

Section: Resultssupporting

confidence: 93%

“…However, in the studies by Wang et.al [ 27 ] and He et al [ 17 ], miR-144-3p and miR-144-5p had VB specificity. However, it was worth noting that the study from Bamberg et al [ 65 ] revealed that miR-144-3p was not able to identify VB and MB. In our study, miR-144-5p and miR-144-3p had high expression levels in both the MB and VB groups ( Figure 6 C), especially miR-144-3p in the MB.…”

Section: Resultsmentioning

confidence: 99%

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A Comprehensive Characterization of Small RNA Profiles by Massively Parallel Sequencing in Six Forensic Body Fluids/Tissue

Liu

Wang

et al. 2022

Genes

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Body fluids/tissue identification (BFID) is an essential procedure in forensic practice, and RNA profiling has become one of the most important methods. Small non-coding RNAs, being expressed in high copy numbers and resistant to degradation, have great potential in BFID but have not been comprehensively characterized in common forensic stains. In this study, the miRNA, piRNA, snoRNA, and snRNA were sequenced in 30 forensic relevant samples (menstrual blood, saliva, semen, skin, venous blood, and vaginal secretion) using the BGI platform. Based on small RNA profiles, relative specific markers (RSM) and absolute specific markers (ASM) were defined, which can be used to identify a specific body fluid/tissue out of two or six, respectively. A total of 5204 small RNAs were discovered including 1394 miRNAs (including 236 novel miRNA), 3157 piRNAs, 636 snoRNAs, and 17 snRNAs. RSMs for 15 pairwise body fluid/tissue groups were discovered by differential RNA analysis. In addition, 90 ASMs that were specifically expressed in a certain type of body fluid/tissue were screened, among them, snoRNAs were reported first in forensic genetics. In brief, our study deepened the understanding of small RNA profiles in forensic stains and offered potential BFID markers that can be applied in different forensic scenarios.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

A Comprehensive Characterization of Small RNA Profiles by Massively Parallel Sequencing in Six Forensic Body Fluids/Tissue

Liu

Wang

et al. 2022

Genes

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“…Recently, Bamberg et al established a simultaneous mRNA/miRNA multiplex assay for body fluid identification [36]. The simultaneous reverse strategy of mRNA and miRNA adopted by us is different from the previous study reported by the Bamberg et al, and the present study has achieved better results to a certain extent.…”

Section: Discussioncontrasting

confidence: 66%

Forensic validation of a combined analysis of mRNA and miRNA markers for precise tissue origin inferences of five kinds of body fluids by RT‐qPCR

Chen,

Xu,

Zhu

2023

Electrophoresis

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Correctly inferring the tissue origin types of forensic‐relevant body fluids left at a crime scene is beneficial for reconstructing a crime scene. However, it is still a challenge to accurately identify different kinds of body fluids at a crime scene. Shorter sequence length and anti‐degradation microRNA (miRNA) can be used to infer the tissue sources of biological fluid traces, but a limited number of miRNAs are tissue specific. The application of messenger RNA (mRNA) has been confirmed by different studies based on its high tissue specificity. According to the differential expression features of mRNA or miRNA in forensically relevant body fluids, this study developed a simultaneously reversed mRNA and miRNA system and then used these two types of RNAs for the determinations of five common kinds of body fluids. Compared with previously reported single kind of mRNA or miRNA assay, the combined mRNA and miRNA system showed good advantages for human body fluid identifications, especially it could be applied in mixed samples. In conclusion, the obtained results indicated that this combined mRNA and miRNA system might provide a scientific and accurate reference for body fluid identifications.

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“…Because of their amplification sizes and tissue specific features, interests on miRNAs are increasing [8]. In a study which simultaneous examination of miRNA and mRNA biomarkers in body fluids, study group stated that miRNA biomarkers are more advantageous when compared with mRNA biomarkers in degraded samples [9].…”

Section: Rna In Forensic Geneticsmentioning

confidence: 99%

RNA-Approached technology applications in forensic genetics

Yükseloğlu¹

2022

NOFOR

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Ribonucleic acid (RNA) is a nucleic acid which is structurally different from DNA. DNA is the most used and approved nucleic acid in routine applications in forensic caseworks. In this article, giving an information about which RNA types are chosen for which forensic applications is aimed. RNA technologies are developing in the concept of forensic genetics and they can be adapted into routine case works in the case of well conditions are supplied. Both coding and non-coding RNAs are investigated for forensic purposes and most examined ones are messenger RNAs, and microRNAs. There are some researches on circular RNAs and piwi-interacting RNAs but they are in low number when compared with first two RNA types. Forensic studies based on RNA technologies are body fluid identification, post mortem interval determination, determination of stain age, estimation of an individual’s age and sex, identification of organ tissues, wound age estimation, and determination of drug abuse. Lastly, different RNA based technologies can be used in these studies and some of them are micro-array, Nano-String technology, real time PCR, end point PCR, high resolution melt (HRM) analysis and next generation sequencing (NGS) technology.

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Best of both: A simultaneous analysis of mRNA and miRNA markers for body fluid identification

Cited by 19 publications

References 39 publications

A Comprehensive Characterization of Small RNA Profiles by Massively Parallel Sequencing in Six Forensic Body Fluids/Tissue

A Comprehensive Characterization of Small RNA Profiles by Massively Parallel Sequencing in Six Forensic Body Fluids/Tissue

Forensic validation of a combined analysis of mRNA and miRNA markers for precise tissue origin inferences of five kinds of body fluids by RT‐qPCR

RNA-Approached technology applications in forensic genetics

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