Bestimmung der Halbwertszeit von Secretin

Lehnert, Peter; Stahlheber, H.; Forell, M. M.; Dost, F. H.; Fritz, H.; Hutzel, M.; Werle, E.

doi:10.1007/bf01484883

Cited by 28 publications

(10 citation statements)

References 5 publications

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“…The half-life of secretin for the initial component of the double exponential function is increased from 1.1 min (gj) before ligature to 2.1 min (g',) after ligature; for the second component it increases from 3.2 min (g2) to 5.3 min (g'2). The mean half-life, as calculated by our method [18,20], increased from 3.1 to 6.2 min after the ligature in this dog.…”

Section: Biological Half-live O F Secretin After Ligature O F Renal Bmentioning

confidence: 93%

“…The methods used for determining the half-life time of secretin have been described elsewhere [18,20].…”

Section: Methodsmentioning

confidence: 99%

“…The mean half-life of secretin [18] increased from 2.9 to 4.8 min after ligature of the renal blood vessels (4 dogs). The half-life for the initial com ponent of the double exponential function is extended from 1.1 to 1.7 min, the half-life for the second component from 3.2 to 4.8 min (same dogs, n = 4).…”

Section: Biological Half-live O F Secretin After Ligature O F Renal Bmentioning

confidence: 99%

“…8). For comparing the halflife of secretin it is necessary to fix certain points [18,20] or to use the method described by M a k h l o u f [22] and R eeder et al [28] calculating the rate of disappearance of gastrin from the circulating blood.…”

Section: Biological Half-live O F Secretin After Ligature O F Renal Bmentioning

confidence: 99%

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Studies on the Elimination of Secretin and Cholecystokinin with Regard to the Kinetics of Exocrine Pancreatic Secretion

Lehnert¹,

Stahlheber²,

Forell³

et al. 1974

Digestion

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Studies were performed on 72 anesthetized mongrel dogs. Upon injection or infusion of secretin into the portal vein, the pancreatic volume and bicarbonate response was found to be significantly smaller than following administration into the femoral vein, but only when low doses of secretin (0.25 clin U/kg/h or smaller) were applied. No difference was observed in pancreatic protein and enzyme secretion after cholecystokinin, when both high and low doses were applied to the portal or femoral vein. The injection of secretin and cholecystokinin into the renal artery causes a loss of biological activity of 75 and 60%, respectively, as compared to femoral administration. Ligature of the blood vessels of both kidneys resulted in an increase in the biological activity of secretin of 80% and of cholecystokinin of 50%. The slopes of the flow rate curves determined upon secretin injection were smaller after ligature of the renal blood vessels. The biological half-life of secretin increased from an average of 2.9 to 4.8 min. These results indicate that the kidneys play a role in the catabolism of exogenous secretin and cholecystokinin, whereas the blood and the liver (at least for cholecystokinin) seem to be of small importance.

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Section: Biological Half-live O F Secretin After Ligature O F Renal Bmentioning

confidence: 93%

“…The methods used for determining the half-life time of secretin have been described elsewhere [18,20].…”

Section: Methodsmentioning

confidence: 99%

Section: Biological Half-live O F Secretin After Ligature O F Renal Bmentioning

confidence: 99%

Section: Biological Half-live O F Secretin After Ligature O F Renal Bmentioning

confidence: 99%

See 2 more Smart Citations

Studies on the Elimination of Secretin and Cholecystokinin with Regard to the Kinetics of Exocrine Pancreatic Secretion

Lehnert¹,

Stahlheber²,

Forell³

et al. 1974

Digestion

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“…Since about the time of the initial isolation of the hormone from hog intestine by Jorpes and Mutt (1959), there have been refinements in bioassay technique (Ivy and Janecek, 1959;Mutt and Soderberg, 1959;Heatley, 1968) which have produced considerable standardisation of results. Unfortunately, these techniques lacked specificity and precision, and were incapable of measuring basal levels of secretin in the circulating plasma of animals and man.Calculations of the disappearance half-time (TI) of secretin were, however, made in laboratory animals using pancreatic secretion as an index of secretin activity (Clark et al, 1967;Lehnert et al, 1969;Hubel, 1972).We have recently validated a radioimmunoassay which is both sensitive and specific and can accurately measure concentrations of secretin of less than 20 pg/mi in plasma (Rayford et al, 1976).Using this assay technique, we have measured plasma levels of secretin in dogs after an infusion of pure natural porcine hormone and have calculated its disappearance half-time. We have also measured basal and stimulated levels of endogenous secretin in dogs and have calculated its disappearance halftime.…”

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confidence: 99%

Disappearance half-time of endogenous and exogenous secretin in dogs.

Curtis¹,

Fender²,

Rayford³

et al. 1976

Gut

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SUMMARY We have used a sensitive and specific radioimmunoassay to measure the disappearance half-times of exogenous porcine secretin and endogenous canine secretin in the dog and found them to be 2-45 and 2-85 minutes, respectively.Since the discovery of secretin in 1902 by Bayliss and Starling, quantitative estimations of secretin activity have been based on the ability of the hormone to stimulate flow of bicarbonate-rich pancreatic juice. Since about the time of the initial isolation of the hormone from hog intestine by Jorpes and Mutt (1959), there have been refinements in bioassay technique (Ivy and Janecek, 1959;Mutt and Soderberg, 1959;Heatley, 1968) which have produced considerable standardisation of results. Unfortunately, these techniques lacked specificity and precision, and were incapable of measuring basal levels of secretin in the circulating plasma of animals and man.Calculations of the disappearance half-time (TI) of secretin were, however, made in laboratory animals using pancreatic secretion as an index of secretin activity (Clark et al., 1967;Lehnert et al., 1969;Hubel, 1972).We have recently validated a radioimmunoassay which is both sensitive and specific and can accurately measure concentrations of secretin of less than 20 pg/mi in plasma (Rayford et al., 1976).Using this assay technique, we have measured plasma levels of secretin in dogs after an infusion of pure natural porcine hormone and have calculated its disappearance half-time. We have also measured basal and stimulated levels of endogenous secretin in dogs and have calculated its disappearance halftime. proximately 20 kg were placed in Pavlov stands and an intravenous catheter placed in a foreleg and a hind leg of each dog. After collecting two 5 ml basal blood samples, 10 minutes apart, each dog received an infusion of pure natural secretin (Professor V.Mutt, Gastrointestinal Hormone Laboratory [GIH], Karolinska Institute, Stockholm), 1.5 unit/kg body weight, dissolved in 50 ml 0-9 % NaCI. This secretin preparation contains 3500 clinical units/mg. The infusion was delivered over 30 minutes into a foreleg vein using a Harvard syringe pump. Blood samples were collected from the hind leg catheter at five-minute intervals during the infusion. The infusion was then terminated and samples collected at one-minute intervals for 10 minutes, then at two-minute intervals for 10 minutes. DISAPPEARANCE HALF-TIME OF ENDOGENOUS SECRETINA further six fasting, adult, mongrel dogs were anaesthetized with intravenous sodium pentobarbital ,(25,mg/kg), and the abdomen opened. A segment of bowel, comprising the whole of the duodenum and the proximal 50 cm of the jejunum, was prepared for stimulation of secretin release by ligation of the pylorus, ligation of the superior pancreaticoduodenal vein at its entry into the portal vein, and division of the jejunum and its mesentery 50 cm distal to the ligament of Treitz. A cannula was introduced into the proximal duodenum for infusion of acid, and the distal end of the segment of bowel was left open to allow f...

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