Bet v 1‐Homologous Allergens

Hoffmann‐Sommergruber, Karin; Radauer, Christian

doi:10.1002/9780470995174.ch8

Cited by 4 publications

(5 citation statements)

References 82 publications

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“…These homologous allergens show a high similarity in primary, secondary and tertiary structure. At the amino acid level, the fruit allergens Mal d 1 and Pru av 1 show 64 -66% sequence identity with Bet v 1, compared to 44% for the homologous vegetable allergens Api g 1 and Dau c 1 [10]. The secondary and tertiary protein structures also exhibit a high degree of similarity, as shown in X-ray crystallization and NMR studies of Bet v 1 and Pru av 1 [11 -13].…”

Section: Introductionmentioning

confidence: 97%

Purification and characterization of natural Bet v 1 from birch pollen and related allergens from carrot and celery

Bollen

García

Cordewener

et al. 2007

Molecular Nutrition Food Res

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Birch pollen allergy is predominantly caused by the major allergen Bet v 1 and can lead to crossreactions with homologous proteins in food. Two major cross-reactive food allergens are Dau c 1 from carrot and Api g 1 from celery, which have never been purified from their natural source. Here, we describe a non-denaturing purification method for obtaining natural Bet v 1, Dau c 1 and Api g 1, comprising of ammonium sulfate precipitation, hydrophobic interaction chromatography and size exclusion chromatography. This method resulted in 98-99% pure isoform mixtures for each allergen. Characterization of these isoform mixtures with Q-TOF MS/MS clearly showed earlier reported isoforms of Bet v 1, Dau c 1 and Api g 1, but also new isoforms. The presence of secondary structure in the three purified allergens was demonstrated via circular dichroism and showed high similarity. The immune reactivity of the natural allergens was compared with recombinant proteins by Western blot and ELISA and showed similar reactivity.

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Section: Introductionmentioning

confidence: 97%

Purification and characterization of natural Bet v 1 from birch pollen and related allergens from carrot and celery

Bollen

García

Cordewener

et al. 2007

Molecular Nutrition Food Res

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“…In soybean, the Bet v 1 homologue SAM22 allergen consists of ten genes present in a tandem array (Crowell et al 1992;KleineTebbe et al 2002). Fourth, Bet v 1-related genes are expressed in different tissues and under biotic or abiotic stresses (Hoffmann-Sommergruber and Radauer 2004). Several expression studies on apple Mal d 1 genes also showed some differences for individual genes.…”

Section: Mal D 1 Gene Family Organization and Evolutionary Originmentioning

confidence: 99%

“…Recombinant by expressed proteins of these isoallergens and variants identified in this study can be used to assess their allergenicity. (Hoffmann-Sommergruber et al 2004). In soybean, the Bet v 1 homologue SAM22 allergen consists of ten genes present in a tandem array (Crowell et al 1992;KleineTebbe et al 2002).…”

Section: Mal D 1 Gene Family Organization and Evolutionary Originmentioning

confidence: 99%

“…Both Bet v 1 and Mal d 1 belong to a group of pathogenesis-related (PR)10 proteins . The PR-10 genes appear abundantly in plants Hoffmann-Sommergruber and Radauer 2004;Liu and Ekramoddoullah 2004). Expression of these genes is induced by stress, such as attack by plant pathogens, and occurs during ripening (Atkinson et al 1996;Puehringer et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Genomic cloning and linkage mapping of the Mal d 1 (PR-10) gene family in apple (Malus domestica)

et al. 2005

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Fresh apples can cause birch pollen-related food allergy in northern and central European populations, primarily because of the presence of Mal d 1, the major apple allergen that is cross-reactive to the homologous and sensitizing allergen Bet v 1 from birch. Apple cultivars differ significantly in their allergenicity. Knowledge of the genetic basis of these differences would direct breeding for hypoallergenic cultivars. The PCR genomic cloning and sequencing were performed on two cultivars, Prima and Fiesta, which resulted in 37 different Mal d 1 gDNA sequences. Based on the mapping of sequence-specific molecular markers, these sequences appeared to represent 18 Mal d 1 genes. Sixteen genes were located in two clusters, one cluster with seven genes on linkage group (LG) 13, and the other cluster with nine genes on the homoeologous LG 16. One gene was mapped on LG 6, and one remained unmapped. According to sequence identity, these 18 genes could be subdivided into four subfamilies. Subfamilies I-III had an intron of different size that was subfamily and genespecific. Subfamily IV consisted of 11 intronless genes. The deduced amino acid sequence identity varied from 65% to 81% among subfamilies, from 82% to 100% among genes within a subfamily, and from 97.5% to 100% among alleles of one gene. This study provides a better understanding of the genetics of Mal d 1 and the basis for further research on the occurrence of allelic diversity among cultivars in relation to allergenicity and their biological functions.

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“…Mal d 1 and Mal d 4 allergens are heat labile and sensitive to proteolytic degradation, while Mal d 2 and Mal d 3 allergens are resistant to heat and stable to proteolytic degradation. Mal d 1 and the Mal d 2 proteins are the most significant apple allergens inducing IgE-mediated hypersensitivity reaction (Hoffmann-Sommergruber & Radauer, 2004).…”

Section: Introductionmentioning

confidence: 99%

Purification of natural Mal d 1 and Mal d 2 allergens and monitoring of their expression levels during ripening in Golden Delicious apple

Szamos

Takács

Szabó

et al. 2011

Food Research International

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Bet v 1‐Homologous Allergens

Cited by 4 publications

References 82 publications

Purification and characterization of natural Bet v 1 from birch pollen and related allergens from carrot and celery

Purification and characterization of natural Bet v 1 from birch pollen and related allergens from carrot and celery

Genomic cloning and linkage mapping of the Mal d 1 (PR-10) gene family in apple (Malus domestica)

Purification of natural Mal d 1 and Mal d 2 allergens and monitoring of their expression levels during ripening in Golden Delicious apple

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