Beta-Adrenergic Blockade Decreases the Neuroimmune Changes in Mice Induced by Cohabitation with an Ehrlich Tumor-Bearing Cage Mate

Abstract

Objectives: Cohabitation with Ehrlich tumor-bearing (ETB) mice induced behavioral, neurochemical, hormonal, and immune effects in the conspecifics as a consequence of stress-induced activation of the sympathetic nervous system (SNS) with catecholamine release. In the current study, the nonspecific β-AR blocker d,l-propranolol and the specific β₂-AR blocker ICI-118.551 were employed as pharmacological tools to assess the extent to which catecholamines participated in the effects induced by cohabitation with ETB mice. Methods: Two experiments were performed, 1 with d,l-propranolol treatment and the other with ICI-118.551. One mouse in the experimental group was called the “companion of the sick partner” (CSP) since it was forced to live in the same cage with 2 (experiment 1) or 1 (experiment 2) cage mate that had been i.p. injected with 5 × 10⁶ Ehrlich tumor cells. Results: The d,l-propranolol treatment, but not the ICI-118.551 treatment, attenuated the effects of cohabitation with 2 ETB mice on both open-field behavior and the hypothalamic levels and turnover rate of norepinephrine. The 2 β-AR blockers were unable to change the serum corticosterone levels and adrenal weights of the CSP mice; however, these drugs abrogated the effects of cohabitation on neutrophil oxidative burst and phagocytosis. Finally, an increase in the 5-HT turnover rate was observed in the olfactory bulb of CSP mice compared to their respective controls, an effect that was not modified by β-AR blockade. Conclusion: These results confirm and strengthen our hypothesis that the SNS is involved in the effects induced by cohabitation with ETB mice and point towards β₂-AR participation in the immune effects analyzed.

Effect of propranolol on temporomandibular joint pain in repeatedly stressed rats

“…We have also shown that propranolol‐induced analgesia occurs as a result of reducing the concentration of pro‐inflammatory mediators in the TMJ region [18]. Studies show that intraperitoneally injected propranolol does not alter the expression of serotonin receptors (5‐HTRs) in the brainstem and central nervous system [35]. Thus, we presume that the levels of 5‐HTRs are unchanged with local injection and at low doses of propranolol.…”

Thimet Oligopeptidase (EC 3.4.24.15) Key Functions Suggested by Knockout Mice Phenotype Characterization

“…For neurochemical analyses tissues were homogenized in 0.1 M perchloric acid by manual sonication, centrifuged at 10,000 rpm for 20 min to remove the supernatant, and stored at −80 °C until the determination of monoamine levels. The levels of neurotransmitters and their metabolites (dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), and serotonin (5HT), 5-hydroxyindoleacetic acid (5HIAA), and noradrenaline (NOR) were measured by reversed-phase high-performance liquid chromatography (HPLC) using a system (model 6A, Shimadzu, Kyoto, Japan) with an electrochemical detector, as described elsewhere [128,129]. Briefly, 20 μL samples were loaded into a sample injector, and the mobile phase was delivered at a constant rate of 1.2 mL/min.…”

Psychological Stress Up-Regulates CD147 Expression Through Beta-Arrestin1/ERK to Promote Proliferation and Invasiveness of Glioma Cells

“…High expression of CD147 leads to increased tumor growth and metastasis in bone cancer cells and glioma, which is related to the induction of MMPs and modification of the tumor microenvironment ( 15 , 16 ). Research has shown that high levels of stress hormones cause neuroimmunoendocrine system disorders ( 17 ), which leads to alterations in the tumor microenvironment ( 18 ). However, our previous study showed that activation of adrenergic receptors promoted the expression of LDHA, MCT1, and MCT4, all of which are directly related to the metabolism of lactic acid in glioma cells ( 19 – 21 ).…”