.BETA.-amylase Variation in Wild Barley Accessions

Zhang, Wensheng; Kaneko, Toshihiro; Takeda, Kazuyoshi

doi:10.1270/jsbbs.54.41

Cited by 7 publications

(7 citation statements)

References 30 publications

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“…By combination of these three thermostability types and three IEF patterns, theoretically there could be nine possible kinds β‐amylase phenotypes (A‐I, A‐Ia, A‐II, B‐I, B‐Ia, B‐II, C‐I, C‐Ia, and C‐II). We have found all these nine types in barley except that the A‐I type, which was only found in wild barley H. vulgare subsp spontaneum (Zhang et al, 2004). This indicated that the thermostability and IEF pattern are independent factors of β‐amylase.…”

Section: Resultsmentioning

confidence: 85%

“…Thell. (Zhang et al, 2004). Among these seven patterns, IIa (OUC1152) and IV (OUU421) were observed for the first time.…”

Section: Resultsmentioning

confidence: 99%

“…This indicated that the thermostability and IEF pattern are independent factors of β‐amylase. Besides, in H. vulgare subsp spontaneum (the ancestor of cultivated barley), the three main thermostability types (A, B, and C) and the three major IEF patterns (I, Ia, and II) of β‐amylase already existed (Zhang et al, 2004). Hordeum vulgare subsp spontaneum had A‐I, A‐II, B‐I, B‐Ia, B‐II, and C‐II six major types, which is one more than the five major types in cultivated barley.…”

Section: Resultsmentioning

confidence: 99%

“…Barley accessions in subsp. spontaneum (Zhang et al, 2004). This indicated the Americas showed the highest diversity (0.796), folthat the thermostability and IEF pattern are indepenlowed by the former USSR accessions (0.765) and Eurodent factors of ␤-amylase.…”

Section: Diversity Index Of ␤-Amylase Phenotypementioning

confidence: 90%

“…The lowest diversity (0.06) was observed in Ethiopian existed (Zhang et al, 2004). Hordeum vulgare subsp.…”

Section: Diversity Index Of ␤-Amylase Phenotypementioning

confidence: 91%

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Differentiation of β‐Amylase Phenotypes in Cultivated Barley

Zhang

Kaneko²,

Ishii

et al. 2004

Crop Science

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β‐Amylase types coded by the Bmy1 locus were investigated by analyzing the thermostability and isoelectric focusing (IEF) patterns in 8270 accessions of cultivated barley (Hordeum vulgare L.) from different regions of the world. The β‐amylase types were classified into three main thermostability types (A, high; B, medium; C, low) and three major IEF patterns (I, Ia, and II) together with several rare mutant types. By combined analysis of the thermostability types and IEF patterns, we found 14 β‐amylase phenotypes. Among them, A‐II, B‐I, B‐Ia, B‐II, and C‐II were confirmed to be the major types comprising more than 99% of cultivated barley, and accessions belonging to types A‐Ia, A‐IIa, C‐I, C‐Ia, and C‐IV were reported here for the first time. We also confirmed a clear geographical differentiation of β‐amylase phenotypes: In East Asia, nearly 90% of the accessions were of the A‐II type, while 97% of the Ethiopian accessions were of the C‐II type. The B‐I type accessions were mostly restricted to the former USSR and Europe. In Nepal, Bhutan, India, and Pakistan, 90% of the B type accessions were of the B‐Ia type. The B‐II type barley seems to have originated in Turkey and then mainly spread to North Africa, Europe, and the former USSR.

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Section: Resultsmentioning

confidence: 85%

“…Thell. (Zhang et al, 2004). Among these seven patterns, IIa (OUC1152) and IV (OUU421) were observed for the first time.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Diversity Index Of ␤-Amylase Phenotypementioning

confidence: 90%

“…The lowest diversity (0.06) was observed in Ethiopian existed (Zhang et al, 2004). Hordeum vulgare subsp.…”

Section: Diversity Index Of ␤-Amylase Phenotypementioning

confidence: 91%

See 3 more Smart Citations

Differentiation of β‐Amylase Phenotypes in Cultivated Barley

Zhang

Kaneko²,

Ishii

et al. 2004

Crop Science

Self Cite

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The Properties and Genetics of Barley Malt Starch Degrading Enzymes

Evans

Li²,

Eglinton

2009

Advanced Topics in Science and Technology in China

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Genetic variation of Bmy1 alleles in barley (Hordeum vulgare L.) investigated by CAPS analysis

Zhang

Liu

2007

Theor Appl Genet

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The enzyme beta-amylase is one of the most important hydrolytic enzymes in the grain of malting barley and is encoded by the gene Bmy1. To learn more about its structure and function, a total of 657 barley accessions including 541 Hordeum vulgare ssp. vulgare (HV), and 116 H. vulgare ssp. spontaneum (HS) were selected for the cleaved amplified polymorphic sequence (CAPS) analysis. These materials, covering all the 16 kinds of beta-amylase phenotypes screened from more than 8,500 accessions of the world barley germplasm, were classified into 13 CAPS types in the present study. A combined assay of phenotypes and CAPS types revealed extensive genetic variation at the Bmy1 locus, and in total 23 Bmy1 allele types were identified. The newly identified alleles (A-I-11, A-II-6, A-II-7, A-II-10, B-I-3, B-I-12 and B-I-13) provided us with a novel resource for barley breeding and Bmy1 study. In HV barley, six out of seven major allele types (C-II-1, B-II-2, B-Ia-3, A-II-5, A-II-6, and A-II-7) were shared with HS barley; the B-I-8 allele, which was predominant in north European cultivated barley, was found to be unique. Remarkably, very low Bmy1 genetic variation was detected in Tibetan barleys, which puts the validity of the hypothesis that Tibet is one of the original centers of cultivated barley into question.

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.BETA.-amylase Variation in Wild Barley Accessions

Cited by 7 publications

References 30 publications

Differentiation of β‐Amylase Phenotypes in Cultivated Barley

Differentiation of β‐Amylase Phenotypes in Cultivated Barley

The Properties and Genetics of Barley Malt Starch Degrading Enzymes

Genetic variation of Bmy1 alleles in barley (Hordeum vulgare L.) investigated by CAPS analysis

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