Beta- and Gamma-Cytoplasmic Actins Are Required for Meiosis in Mouse Oocytes1

Brockmann, Céline; Huarte, Joachim; Dugina, Vera B.; Challet, Ludivine; Rey, Emmanuelle; Conne, Béatrice; Swetloff, Adam; Nef, Serge; Chaponnier, Christine; Vassalli, Jean‐Dominique

doi:10.1095/biolreprod.111.091736

Cited by 25 publications

(18 citation statements)

References 38 publications

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“…Cytoplasmic actin isoforms play unique roles in many processes, i.e., regulation of structure and permeability of epithelial junctions (Baranwal et al 2012) or meiosis of oocytes (Brockmann et al 2011). There is also some evidence that β- and γ-actins are located in different cytoplasmic areas (Khaitlina 2001).…”

Section: Introductionmentioning

confidence: 99%

Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells

Simiczyjew

Mazur

Popow-Woźniak

et al. 2014

Histochem Cell Biol

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Actins are eukaryotic proteins, which are involved in diverse cellular functions including muscle contraction, cell motility, adhesion and maintenance of cell shape. Cytoplasmic actin isoforms β and γ are ubiquitously expressed and essential for cell functioning. However, their unique contributions are not very well understood. The aim of this study was to determine the effect of β- and γ-actin overexpression on the migration capacity and actin cytoskeleton organization of human colon adenocarcinoma BE cells. In cells overexpressing β- or γ-actin, distinct cytoskeletal actin rearrangements were observed under the laser scanning confocal microscope. Overexpressed actins localized at the submembranous region of the cell body, especially near to the leading edge and on the tips of pseudopodia. The cells transfected with plasmids containing cDNA for β- or γ-actin were characterized by increased migration and invasion capacities. However, the migration velocity was statistically significantly higher only in the case of γ-actin overexpressing cells. In conclusion, the increased level of β- or γ-actin leads to actin cytoskeletal remodeling followed by an increase in migration and invasion capacities of human colon BE cells. These data suggest that expression of both actin isoforms has an impact on cancer cell motility, with the subtle predominance of γ-actin, and may influence invasiveness of human colon cancer.

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Section: Introductionmentioning

confidence: 99%

Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells

Simiczyjew

Mazur

Popow-Woźniak

et al. 2014

Histochem Cell Biol

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“…However, it was demonstrated previously, that β- and γ-actin isoforms are able to fulfill different functions, e.g. in cell motility [13,22,23,31,74,75], cell division [24], gene transcription [76], endothelial cell motility and neovessel maintenance [77] as well as in developmental processes [78,79]. …”

Section: Discussionmentioning

confidence: 99%

“…The authors postulated that β-actin is essential for cell attachment and contraction, whereas γ-actin is involved in cell motility [22,23]. It is well known that they play unique roles in many physiological processes including regulation of meiosis [24] and permeability of epithelial junctions [25], but due to the above-described controversies, the role of non-muscle β- and γ-actin isoforms in cell migration is still being examined. There were conducted some studies based on both actin isoforms’ silencing, but either only one isoform was knocked down [26,27] or overexpressed [19], or the studies focused on normal cells [22,28], which did not provide a clear answer to the question of their functional diversification in cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Involvement of β- and γ-actin isoforms in actin cytoskeleton organization and migration abilities of bleb-forming human colon cancer cells

et al. 2017

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Amoeboid movement is characteristic for rounded cells, which do not form strong adhesion contacts with the ECM and use blebs as migratory protrusions. It is well known that actin is the main component of mature forms of these structures, but the exact role fulfilled by non-muscle actin isoforms β- and γ- in bleb formation and migration of these cells is still not fully understood. The aim of this study was to establish the role of β- and γ-actin in migration of bleb-forming cancer cells using isoform-specific antibodies and expression of fluorescently tagged actin isoforms. We observed, after staining with monoclonal antibodies, that both actins are present in these cells in the form of a cortical ring as well as in the area of blebs. Additionally, using simultaneous expression of differentially tagged β- and γ-actin in cells, we observed that the actin isoforms are present together in a single bleb. They were involved during bleb expansion as well as retraction. Also present in the area of these protrusions formed by both isoforms were the bleb markers–ezrin and myosin II. The overexpression of β- or γ-actin led to actin cytoskeletal rearrangement followed by the growth of migration and invasion abilities of examined human colon cancer cells, LS174T line. In summary these data prove that both actin isoforms have an impact on motility of bleb-forming cancer cells. Moreover, we conclude that monoclonal antibodies directed against actin isoforms in combination with the tagged actins are good tools to study their role in important biological processes.

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“…Предвари-тельные исследования организации и распределения β-и γ-изоформ актина в митотическом процессе [51,56], а также в мейотических делениях клетки и ранних эмбриональных делениях [57] выявили существенные морфофункциональные различия. Изменение функции γ-актина с помощью микроинъекции изоформ -спе-цифических антител -показало, что γ-актин выполня-ет основную и специфическую функции в установле-нии и / или поддержании асимметрии в первом делении мейоза и поддержании кортикальной целостности.…”

Section: функции изоформ актинаunclassified