Beta-carotene reduces oxidative stress, improves glutathione metabolism and modifies antioxidant defense systems in lead-exposed workers

Kasperczyk, Sławomir; Dobrakowski, Michał; Kasperczyk, Janusz; Ostałowska, Alina; Zalejska–Fiolka, Jolanta; Birkner, Ewa

doi:10.1016/j.taap.2014.07.006

Cited by 67 publications

(43 citation statements)

References 30 publications

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“…Acid (0.1 mM) to cells exposed to Pb 1 mg/L. Reduction of ROS by β‐carotene is going in agreement with Kasperczyk and others (), who observed reduction of ROS in lead exposed workers after β‐carotene supplementation in their diets.…”

Section: Resultssupporting

confidence: 89%

Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by β‐carotene and Ascorbic Acid in Human HepG2 Cells

Darwish

Ikenaka

Nakayama

et al. 2015

Journal of Food Science

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Lead (Pb) is an environmental pollutant that can get entry into human body through contaminated foods, drinks, and inhaled air leading to severe biological consequences, and has been responsible for many deaths worldwide. The objectives of this study were 1st to investigate the modulatory effects of environmentally relevant concentrations of Pb on AhR gene battery, which is controlling xenobiotics metabolism. 2nd, trials to reduce Pb‐induced adverse effects were done using some phytochemicals like β‐carotene or ascorbic acid. Human hepatoma (HepG2) cell lines were exposed to a wide range of Pb concentrations varying from physiological to toxic levels (0 to 10 mg/L) for 24 h. High Pb concentrations (1 to 10 mg/L) significantly reduced phase I (CYP1A1 and 1A2) and phase II (UGT1A6 and NQO1) xenobiotic metabolizing enzyme mRNA expression in a mechanistic manner through the AhR regulation pathway. Additionally, these Pb concentrations induced oxidative stress in HepG2 cells in terms of production of reactive oxygen species (ROS) and induced heme oxygenase‐1 mRNA expression in a concentration‐dependent phenomenon. Coexposure of HepG2 cells to physiological concentrations of some micronutrients, like β‐carotene (10 μM) or ascorbic acid (0.1 mM), along with Pb (1 mg/L) for 24 h significantly reduced the levels of ROS production and recovered AhR mRNA expression into the normal levels. Thus, consumption of foods rich in these micronutrients may help to reduce the adverse effects of lead in areas with high levels of pollution.

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Section: Resultssupporting

confidence: 89%

Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by β‐carotene and Ascorbic Acid in Human HepG2 Cells

Darwish

Ikenaka

Nakayama

et al. 2015

Journal of Food Science

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“…Our study has proven that the liver functional markers (AST, ALT, ALP, and Bilirubin) show significant elevation upon administration of INH and RIF. Beta-carotene is also a known antioxidant as seen previously by its reduction of oxidative stress along with rejuvenation of antioxidant defense systems in lead exposed workers [Kasperczyk et al, 2014]. This shows that the liver undergoes acute injury due to ATDs as witnessed in previous studies [Sodhi et al, 1997].…”

Section: Discussionsupporting

confidence: 65%

Comparative Modulation of Levels of Oxidative Stress in the Liver of Anti‐Tuberculosis Drug Treated Wistar Rats by Vitamin B12, Beta‐Carotene, and Spirulina fusiformis: Role of NF‐κB, iNOS, IL‐6, and IL‐10

Martin

Sabina

2017

J of Cellular Biochemistry

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Isoniazid (INH) and Rifampicin (RIF) are known hepatotoxic agents. We compared the efficacy of Spirulina fusiformis and its active components vitamin B12 and beta-carotene in attenuating INH and RIF induced hepatotoxicity. We also tried to elucidate the inflammatory mechanism behind anti-tuberculosis drug induced hepatotoxicity. INH and RIF were administered to Wistar albino rats for 28 days to induce hepatotoxicity. S. fusiformis, vitamin B12, and beta-carotene were co-administered with INH and RIF and their hepatoprotective, antioxidant, and immunomodulatory roles were studied through blood and liver analysis. Changes induced by INH and RIF in antioxidants, cytokines (IL-6 and IL-10) and expression of Nuclear Factor-κB (NF-κB) and Nitric Oxide Synthase (iNOS) were also studied. Supplement treatment caused restoration of liver function parameters to normal levels along with reversal of inflammatory changes in IL-6 and IL-10 levels. Liver PCNA, iNOS, and NF-κB expression were reduced in the supplement treated tissues compared to INH and RIF treated rats as evidenced by immunohistochemistry and quantitative PCR. Correlation of IL-6 levels, PCNA, and iNOS with NF-κB showed its pivotal role in the inflammatory process. Study shows the pivotal role of NF-kB and the equivalence in antioxidant efficacy of vitamin B12 and beta-carotene compared to Spirulina fusiformis. J. Cell. Biochem. 118: 3825-3833, 2017. © 2017 Wiley Periodicals, Inc.

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“…Therefore, under oxidative stress conditions, when the GSH pool is depleted, uric acid may induce endothelial dysfunction (Gersch et al 2008; Xie et al 2015). It is well documented that lead exposure induces GSH depletion (Kasperczyk et al 2014). In light of this, the role of uric acid in the defense against lead toxicity can be divergent and may depend on the specific redox conditions of the particular microenvironment.…”

Section: Discussionmentioning

confidence: 99%

“…The development of oxidative stress in chronic lead exposure is well established (Kasperczyk et al 2014). The decrease in thiol group level and the elevation of TOS and OSI values observed in the present study confirm that a subchronic exposure to lead is also able to induce oxidative stress despite increased TAC value.…”

Section: Discussionmentioning

confidence: 99%

Association between subchronic and chronic lead exposure and levels of antioxidants and chemokines

Dobrakowski

Kasperczyk

Pawlas

et al. 2016

Int Arch Occup Environ Health

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PurposeThis study aimed to compare the influence of lead on the non-enzymatic antioxidant defenses and the levels of chemokines in workers subchronically and chronically exposed to lead.MethodsThe study population was divided into three groups. The first group consisted of male workers subchronically exposed to lead for 40 ± 3.2 days, while the second group included male workers chronically exposed to lead. The third group was a control group.ResultsThe levels of uric acid and bilirubin were significantly higher after a subchronic exposure to lead compared to the baseline by 22 and 35 %, respectively. Similarly, the values of total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) increased by 15, 50, and 33 %, respectively. At the same time, the levels of thiol groups and albumin decreased by 5 and 8 %, respectively. Additionally, the levels of interleukin-8 (IL-8) and macrophage inflammatory protein-1β (MIP-1β) were significantly higher after a subchronic exposure to lead compared to the baseline by 34 and 20 %, respectively. Moreover, IL-8 level was significantly higher by 40 % in the group of workers chronically exposed to lead than in the control group, while the level of interferon gamma-induced protein-10 (IP-10) was significantly lower by 28 %.ConclusionsSimilar to chronic lead exposure, subchronic exposure to lead is associated with elevated blood levels of uric acid and bilirubin in humans. This probably results in increased TAC value despite thiol depletion. However, the compensatory activation of non-enzymatic antioxidant defenses seems to be insufficient to protect against lead-induced oxidative stress, which may be additively enhanced by the pro-inflammatory action of chemokines, especially IL-8.

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Beta-carotene reduces oxidative stress, improves glutathione metabolism and modifies antioxidant defense systems in lead-exposed workers

Cited by 67 publications

References 30 publications

Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by β‐carotene and Ascorbic Acid in Human HepG2 Cells

Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by β‐carotene and Ascorbic Acid in Human HepG2 Cells

Comparative Modulation of Levels of Oxidative Stress in the Liver of Anti‐Tuberculosis Drug Treated Wistar Rats by Vitamin B12, Beta‐Carotene, and Spirulina fusiformis: Role of NF‐κB, iNOS, IL‐6, and IL‐10

Association between subchronic and chronic lead exposure and levels of antioxidants and chemokines

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