Beta Carotene Treatment and Erythrocytic Protoporphyrin Levels

Miao, Lillian Lin; Mathews-Roth, Micheline M.; Poh–Fitzpatrick, Maureen B.

doi:10.1001/archderm.1979.04010070004007

Cited by 13 publications

(1 citation statement)

References 7 publications

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“…When erythropoietic protoporphyria (EPP) red cells were layered on top of a monolayer of tissue‐cultured cells and exposed to light, PpIX was detached from hemoglobin inside the EPP cells and transferred to their plasma membrane and further to neighboring cells (8). This probably explains why the PpIX concentration in erythrocytes of EPP patients is lower in the summer season than in the winter season (9) and the observation that these patients experience a much higher skin photosensitivity on the day after a day of mild light‐induced cutaneous symptoms (10). Thus, the first light exposure may have transferred some PpIX from EPP erythrocytes to endothelial cells in the skin.…”

Section: Introductionmentioning

confidence: 99%

Transfer of Protoporphyrin IX Between Cells¶

Moan¹,

Wei²,

Stokke³

2002

Photochemistry and Photobiology

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Human adenocarcinoma cells of the line WiDr and human leukemia T cells of the line Jurkat were incubated with 5aminolevulinic acid and found to produce protoporphyrin IX (PpIX). They were able to transfer a fraction of the sensitizer to neighboring control cells. The transfer took place through direct membrane contact. Light exposures, inactivating about 20% of the sensitized cells, did not result in any acceleration of the transfer of PpIX. This is in contrast to what has been reported for PpIX in erythrocytes from patients with erythropoietic protoporphyria. In these cells light exposure transfers PpIX from the binding sites on hemoglobin to the plasma membrane and further to neighboring cells. The lack of light-induced transfer in the WiDr and Jurkat cells may be related to the binding sites of PpIX, supposedly membrane lipids and proteins embedded therein. Light exposure slightly increased the rate of loss of PpIX from WiDr cells.

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Section: Introductionmentioning

confidence: 99%