Beta-catenin and p53 expression in topographic compartments of colorectal cancer and its prognostic value following surgery

Prieto, J.; Álvarez, Martina; Hierro, María Isabel; García, Isabel; Vicioso, Luís

doi:10.1016/j.anndiagpath.2017.05.014

Cited by 2 publications

(2 citation statements)

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“…β-Catenin is necessary in the differentiation of mesenchymal precursors into OB cells or chondrocytes . The content of the β-catenin protein in normal cells is generally very low, and the location is mainly in the cytoplasm; however, clinical studies have reported that β-catenin was expressed highly in many malignant tumors . In this research, the effect of F on the β-catenin protein expression in mouse OB cells was detected by a laser confocal technique.…”

Section: Discussionmentioning

confidence: 93%

“…28 The content of the β-catenin protein in normal cells is generally very low, and the location is mainly in the cytoplasm; however, clinical studies have reported that β-catenin was expressed highly in many malignant tumors. 29 In this research, the effect of F on the βcatenin protein expression in mouse OB cells was detected by a laser confocal technique. This study observed that 10 −6 mol/ L NaF could significantly upregulate the expression of βcatenin and promote the aggregation of β-catenin in the nucleus.…”

Section: ■ Discussionmentioning

confidence: 99%

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Influence of Calcium Supplementation against Fluoride-Mediated Osteoblast Impairment in Vitro: Involvement of the Canonical Wnt/β-Catenin Signaling Pathway

Wang

Yang

Cheng

et al. 2019

J. Agric. Food Chem.

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Fluoride (F) is capable of promoting abnormal proliferation and differentiation in primary cultured mouse osteoblasts (OB cells), although the underlying mechanism responsible remains rare. This study aimed to explore the roles of wingless and INT-1 (Wnt) signaling pathways and screen appropriate doses of calcium (Ca 2+ ) to alleviate the sodium fluoride (NaF)-induced OB cell toxicity. For this, we evaluated the effect of dickkopf-related protein 1 (DKK1) and Ca 2+ on mRNA levels of wingless/integrated 3a (Wnt3a), low-density lipoprotein receptor-related protein 5 (LRP5), dishevelled 1 (Dv1), glycogen synthase kinase 3β (GSK3β), β-catenin, lymphoid enhancer binding factor 1 (LEF1), and cellular myelocytomatosis oncogene (cMYC), as well as Ccnd1 (Cyclin D1) in OB cells challenged with 10 −6 mol/L NaF for 24 h. The demonstrated data showed that F significantly increased the OB cell proliferation rate. Ectogenic 0.5 mg/L DKK1 significantly inhibited the proliferation of OB cells induced by F. The mRNA expression levels of Wnt3a, LRP5, Dv1, LEF1, β-catenin, cMYC, and Ccnd1 were significantly increased in the F group, while significantly decreased in the 10 −6 mol/L NaF + 0.5 mg/L DKK1 (FY) group. The mRNA expression levels of Wnt3a, LRP5, β-catenin, and cMYC were significantly decreased in the 10 −6 mol/L NaF + 2 mmol/L CaCl 2 (F+CaII) group. The protein expression levels of Wnt3a, Cyclin D1, cMYC, and β-catenin were significantly increased in the F group, whereas they were decreased in the F+CaII group. However, the mRNA and protein expression levels of GSK3β were significantly decreased in the F group while significantly increased in the F+CaII group. In summary, F activated the canonical Wnt/β-catenin pathway and changed the related gene expression and β-catenin protein location in OB cells, promoting cell proliferation. Ca 2+ supplementation (2 mmol/L) reversed the expression levels of genes and proteins related to the canonical Wnt/β-catenin pathway.

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Section: Discussionmentioning

confidence: 93%

Section: ■ Discussionmentioning

confidence: 99%