Beta-glucan induced trained immunity is associated with changes in gut Nccrp-1+ and Mpeg-1+cell populations in rag1-/- zebrafish

Peterman, Beth; Petrie‐Hanson, Lora

doi:10.37722/jambe.2021101

Cited by 2 publications

(8 citation statements)

References 24 publications

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“…Furthermore, vaccination with Y. ruckeri provided the same protection to subsequent E. ictaluri challenge as to subsequent Y. ruckeri challenge [ 29 ]. Further, teleost innate immune cells were shown to mediate protective immunity in the T and B cell deficient rag1 −/− zebrafish [ 2 , 3 ].…”

Section: Discussionmentioning

confidence: 99%

“…Increased phagocytosis following secondary heterologous exposure is a functional characteristic of trained immune cells [ 7 ]. Beta glucan induced trained immunity associated with macrophage populations in rag1 −/− zebrafish [ 3 ]. Beta glucan induced increased phagocytosis in primary carp macrophage cultures [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

“…Edwardsiella piscicida field isolate S11-285 [ 56 ] and Edwardsiella ictaluri field isolate 2003C [ 57 ] were prepared following routine procedures in our lab [ 3 , 57 ]. Fish were challenged by IP injection of E. piscicida (3 × 10 4 CFU/fish) or E. ictaluri (2.5 × 10 4 CFU/fish).…”

Section: Methodsmentioning

confidence: 99%

“…Anterior kidney (ak) leukocytes were isolated following routine procedures in our lab [ 3 , 33 , 58 ]. Five fish were used per treatment with four technical replicates per biological replicate.…”

Section: Methodsmentioning

confidence: 99%

“…The ability of innate immune cells of fish to mediate enhanced protective immunity for one month in the absence of lymphocyte based immunity was shown using rag1 −/− zebrafish and adoptive cell transfers [ 2 ]. Further use of the rag1 −/− model demonstrated long-term protection one month after oral bacterial exposure [ 3 ]. Trained immune mechanisms were demonstrated in carp using in vitro studies with head-kidney derived macrophages [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish

Petrie‐Hanson¹,

Peterman²

2022

Pathogens

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Beta glucan exposure induced trained immunity in channel catfish that conferred long-term protection against Edwardsiella ictaluri and Edwardsiella piscicida infections one month post exposure. Flow cytometric analyses demonstrated that isolated macrophages and neutrophils phagocytosed higher amounts of E. ictaluri and E. piscicida. Beta glucan induced changes in the distribution of histone modifications in the monomethylation and trimethylation of H3K4 and modifications in the acetylation and trimethylation of H3K27. KEGG pathway analyses revealed that these modifications affected expressions of genes controlling phagocytosis, phagosome functions and enhanced immune cell signaling. These analyses correlate the histone modifications with gene functions and to the observed enhanced phagocytosis and to the increased survival following bacterial challenge in channel catfish. These data suggest the chromatin reconfiguration that directs trained immunity as demonstrated in mammals also occurs in channel catfish. Understanding the mechanisms underlying trained immunity can help us design prophylactic and non-antibiotic based therapies and develop broad-based vaccines to limit bacterial disease outbreaks in catfish production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish

Petrie‐Hanson¹,

Peterman²

2022

Pathogens

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Progress and perspective of trained immunity in teleost fish

You,

Yang,

Wang

et al. 2023

Reviews in Aquaculture

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Trained immunity defines the long‐term memory of innate immunity based on innate immune signalling, metabolic changes and epigenetic rewiring of trained innate immune cells, which are characterised by elevated inflammatory responses towards secondary stimuli. The activation of this non‐specific protection‐mediated memory in innate immune cells was identified to benefit host defences as well as play a detrimental role in inflammatory diseases. In this review, we focused on summing up the current progress about the inducers, effector cells, molecular mechanisms and protective effects of trained immunity that have been identified in both mammals and teleost fish, and discussing the future research and application of trained immunity in aquaculture. Revealing the mechanisms of trained immunity will not only provide a novel aspect for understanding the evolution of innate immune memory and their longer‐term protections, but also contribute to the rational design and evaluation of immunopotentiators, adjuvants and potential vaccines in aquaculture.

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Beta-glucan induced trained immunity is associated with changes in gut Nccrp-1+ and Mpeg-1+cell populations in rag1-/- zebrafish

Cited by 2 publications

References 24 publications

Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish

Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish

Progress and perspective of trained immunity in teleost fish

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