beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

Abstract

We have developed a gene-fusion system based on the Escherichia coli (-glucuronidase gene (uidA). The

Two distinct populations of prestalk cells within the tip of the migratory Dictyostelium slug with differing fates at culmination

“…In Fig. 2 we show the staining pattern for the intact promoter using a novel method of double enzymatic staining, where prespore cells are stained by virtue of their expression of the the β-glucuronidase gene (Jefferson et al, 1986) via a prespore-specific promoter (psA:gus), and pstA cells, expressing the ecmA:lacZ fusion gene, are stained with a red substrate. As expected , the level of expression is higher in pstA cells than in pstO cells, at this time of staining there being no detectable expression within the pstO cells.…”

Deletion Analysis of the Catalase-Encoding Gene (catB) Promoter fromAspergillus oryzae

“…A. oryzae transformants were cultured in 200 ml DPY at 30 C for 72 h with shaking. Preparation of cell-free extracts was done as described by Ishida et al 12) The -glucuronidase activity of cell-free extract was measured according to Jefferson et al 13) Protein concentration was determined using a protein assay kit (Bio-Rad Laboratories, Hercules, CA) with BSA as a standard.…”

Targeted manipulation of leaf form via local growth repression

“…For GUS histochemical analysis, plants were pre-treated with 90% ice-cold acetone, and further assay was performed as described by Jefferson et al (1986). The substrate solution contained 5 mM each of potassium ferricyanide and ferrocyanide.…”