Beta-mangostin demonstrates apoptogenesis in murine leukaemia (WEHI-3) cells in vitro and in vivo

Abstract

BackgroundBeta-mangostin (BM) is a xanthone-type of natural compound isolated from Cratoxylum arborescens. This study aimed to examine the apoptosis mechanisms induced by BM in a murine monomyelocytic cell line (WEHI-3) in vitro and in vivo. MethodsA WEHI-3 cell line was used to evaluate the cytotoxicity of BM by MTT. AO/PI and Hoechst 33342 dyes, Annexin V, multiparametric cytotoxicity 3 by high content screening (HCS); cell cycle tests were used to estimate the features of apoptosis and BM effects. Caspase 3 and 9 activities, ROS, western blot for Bcl2, and Bax were detected to study the mechanism of apoptosis. BALB/c mice injected with WEHI-3 cells were used to assess the apoptotic effect of BM in vivo.ResultsBM suppressed the growth of WEHI-3 cells at an IC50value of 14 ± 3 μg/mL in 24 h. The ROS production was increased inside the cells in the treated doses. Both caspases (9 and 3) were activated in treating WEHI-3 cells at 24, 48 and 72 h. Different signs of apoptosis were detected, such as cell membrane blebbing, DNA segmentation and changes in the asymmetry of the cell membrane. Another action by which BM could inhibit WEHI-3 cells is to restrain the cell cycle at the G1/G0 phase. In the in vivo study, BM reduced the destructive effects of leukaemia on the spleen and liver by inducing apoptosis in leukaemic cells.ConclusionBM exerts anti-leukaemic properties in vitro and in vivo.

Subtype Classification, Immune Infiltration, and Prognosis Analysis of Lung Adenocarcinoma Based on Pyroptosis-Related Genes

“…BAK1 is a member of the Bcl-2 family known to interact with the mitochondria to induce apoptosis [ 31 ]. BAK1 is transferred to the mitochondria after receiving a stimulatory signal that induces apoptosis where it induces pore creation in the outer mitochondrial membrane (MOM) through oligomerization increasing permeability and dysregulation of the electron transport chain [ 32 ]. This induces the release of various apoptotic factors, including cytochrome c, into the cytoplasm where they activate caspase-9, which is then processed and activated by the caspase-3 [ 33 ].…”

Bioactivities of β-mangostin and its new glycoside derivatives synthesized by enzymatic reactions

“…The sugar moiety of compound (1) was 2-deoxyglucose as the signals of protons of C-2 0 appeared at δ 2.27 and 1.35 ppm (table 1) [20]. This was also a different signal from the proton signal of the glucose moiety of compound (2). Therefore, compound ( 1 royalsocietypublishing.org/journal/rsos R. Soc.…”

“…Unlike α-mangostin, so far, there have been few studies on the biological activity of β-mangostin and its new derivatization. There are a few reports on the anti-cancer activity of β-mangostin, such as growth inhibition in MCF-7 breast cancer cells [1], murine leukaemia cells (WEHI-3) [2], inhibition of migration and invasion of human hepatocellular carcinoma (HCC) cells [3] and oral squamous cell carcinoma [4]. To the best of our knowledge, there are no reports on the α-glucosidase-inhibitory activity of β-mangostin.…”

Beta-mangostin from Cratoxylum arborescens activates the intrinsic apoptosis pathway through reactive oxygen species with downregulation of the HSP70 gene in the HL60 cells associated with a G0/G1 cell-cycle arrest

“…15 In addition, it showed a cytotoxic effect against the murine leukemia (WEHI-3) cells. 16 The in-depth investigation in this study was conducted to examine the apoptosis induction mechanisms via BM in HL60 cell line in vitro.…”