Betalain exerts protective effect against glaucoma is majorly through the association of inflammatory cytokines

Abstract

The present research aimed at evaluating the protective role of betalain on the in vitro glaucoma model using PC12 neuronal cells. The cultured neuronal cells in a customized pressurized chamber were analyzed for the onset of glutathione, myeloperoxidase (MPO), cathepsin, expression of inflammatory enzymes such as cyclooxygenase (COX-1), lipooxygenase (5- LOX), sPLA2 caveolin-1, glaucoma markers and other inflammatory cytokines in the presence and absence of betalain. The results shown that a significant increase in the expression of oxidative stress with increased activity of cathepsin B and D. On the other hand, the activity of inflammatory enzymes such as COX-1, 5- LOX, sPLA2 were significantly increased in pressure exposed cells. In addition, glaucoma simulated cells demonstrated cells displayed a significant increase in the VEGF, TGF-β, BDGF and neuroserpin compared to control. Moreover, cells predisposed to hydrostatic pressure demonstrated an increase in (p<0.01) inflammatory cytokines such as IL-6, CXCR4, IL-17, IL-1β, and TNF-α levels. However, cells pre-treated with betalain improved the glutathione levels with attenuated MPO activity. Simultaneously, the levels of inflammatory cytokines and other glaucoma marker genes were found to restore in drug pre-treated cells. Thus, the results of the present study demonstrate that the use of betalain on ocular cells can prevent the progression of the disease that can be a suggestive therapeutic for controlling glaucoma like conditions.

Desenvolvimento De Produtos Inovadores Para a Bacia Leiteira De Afrânio-Pe, Com Vista À Ampliação De Mercado

“…Índice Remissivo Emulsão 101,103,110,131,132,141,212,214,215 Estresse 37,42,43,44,64,111,112,113,114,115,117,118,119,120,122,123,124,126 Estresse oxidativo 37, 42,43,44 F Fermentação 29,30,31,32,33,34,35,36,101,172,182,188,189,196,197,198,199,200,201,202,203,204,205,208 Fibras 86,87,92,93,96,97,…”

Os Efeitos Do Uso De Prebióticos E Probióticos Na Hipertensão: Revisão Integrativa Da Literatura

“…A diminuição da atividade da enzima mieloperoxidade (MPO) (que representa a infiltração de neutrófilos) e dos níveis de óxido nítrico (indicador de dano causado por estresse oxidativo) no tecido demonstram a atividade antioxidante e antiinflamatória do extrato de beterraba. Outros trabalhos na literatura confirmam estes resultados 32,33 e atribuem essas propriedades as betalaínas 34,20 .…”

“…Índice Remissivo E Emulsão 101,103,110,131,132,141,212,214,215 Estresse 37,42,43,44,64,111,112,113,114,115,117,118,119,120,122,123,124,126 Estresse oxidativo 37, 42,43,44 F Fermentação 29,30,31,32,33,34,35,36,101,172,182,188,189,196,197,198,199,200,201,202,203,204,205,208 Fibras 86,87,92,93,96,97,<...>…”