Betaxanthin formation and free amino acids in hairy roots of Beta vulgaris var. lutea depending on nutrient medium and glutamate or glutamine feeding

Böhm, H.; Mäck, Gisela

doi:10.1016/j.phytochem.2004.03.008

Cited by 24 publications

(14 citation statements)

References 28 publications

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“…Precursor feeding strategy was used to enhance the biosynthesis of secondary metabolites in hairy root cultures of Catharanthus roseus and Beta vulgaris [31,32]. Isoflavones daidzein and genistein are originated from phenylalanine, a upstream metabolic precursor through phenylpropanoid pathway.…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Shinde

Malpathak

Fulzele

2009

Biotechnol Bioproc E

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^Äëíê~Åí= The effect of biotic elicitors (yeast extract, chitosan), signaling molecule (salicylic acid), and polyamines (putrescine and spermidine) was studied with respect to isoflavones accumulation in hairy root cultures of mëçê~äÉ~=ÅçêóäáÑçäá~=L. Untreated hairy roots (control) accumulated 1.55% dry wt of daidzein and 0.19% dry wt of genistein. In precursor feeding experiment, phenylalanine at 2 mM concentration led to 1.3 fold higher production of daidzein (1.91% dry wt) and genistein (0.27% dry wt). In biotic elicitors, chitosan (2 mg/L) was found to be the most efficient elicitor to induce daidzein (2.78% dry wt) and genistein (0.279% dry wt) levels in hairy roots. Salicylic acid at 1 mM concentration stimulated the maximum accumulation of daidzein (2.2% dry wt) and genistein (0.228% dry wt) 2 days after elicitation. In case of polyamines, putrescine (50 mM) resulted in highest accumulation of daidzein (3.01% dry wt) and genistein (0.227% dry wt) after 5 days of addition. Present results indicated the effectiveness of elicitation and precursor feeding on isoflavones accumulation in hairy roots of mK=ÅçêóäáÑçäá~. This is the first report of elicitation on isoflavones production by hairy roots of mK=ÅçêóäáÑçäá~. © KSBB hÉóïçêÇëW=Psoralea corylifoliaI=éÜóíçÉëíêçÖÉåëI=ÉäáÅáí~íáçåI=éêÉÅìêëçêI=ëáÖå~äáåÖ=ãçäÉÅìäÉ

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Section: Discussionmentioning

confidence: 99%

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Shinde

Malpathak

Fulzele

2009

Biotechnol Bioproc E

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“…Red and yellow B. vulgaris varieties and their hairy root cultures are potential sources of valuable watersoluble nitrogenous pigments, betacyanins and betaxanthins. Many investigations on hairy root cultures of red beet have been reported, in which the effects of inoculum, type and age, medium compositions, amino acid feeding, culture conditions and elicitations on growth of the hairy root cultures and betalains biosynthesis were studied (Hempel and Böhm 1997;Kobayashi et al 2001;Böhm and Mäck 2004;Pavlov et al 2003Pavlov et al , 2005aPavlov and Bley 2006).…”

Section: Hairy Root Culture Technologymentioning

confidence: 99%

Betalains in the era of global agri-food science, technology and nutritional health

et al. 2008

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Natural pigments from plants are of growing interest as substitutes for synthetic dyes in the food and pharmaceutical industry and they increase their added value if they possess positive effects on health. These pigments can be added as such if they are in the legal authorized lists of additives or can be added as phytochemical-enriched plant extract achieving the original product, which has received it, the new nomenclature of functional food. In this way, we comprise on this review a wide point of view of a group of natural pigments known as betalains. From a chemical point of view, betalains are ammonium conjugates of betalamic acid with cyclo-DOPA (betacyanins, violet) and aminoacids or amines (betaxanthins, orange or yellow), which are compounds present in our diet. Besides and taking into account that one type of betalain, betanin is approved as food colorant (E-162) by the European Union and that enlarges the specific weight of these compounds in the diet, we have evolved an overview from the biosynthesis, technology and promoting production, industrial uses as pigments up to physiological and nutritional biovailability or biological and health-promoting properties of betalains for accessible information to industrials, researchers and consumers.

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“…Lutea hairy root culture with S-glutamate does not lead to the formation of the corresponding Vulgaxanthin II, indicating that levels of the amino acids are not the only factors influencing their reactions with betalamic acid (Böhm and Mäck 2004). Adding DOPA to the nutrient medium does not increase the betactyanin contents in cell suspensions of C. rubrum L., but adding tyrosine after the fifth day of cultivation increases them between 50 and 100% (Berlin et al 1986).…”

Section: Growth Regulators and Precursorsmentioning

confidence: 93%

Betalain production in plant in vitro systems

et al. 2008

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Betalains have been widely used as natural colorants for many centuries, but their attractiveness for use as colorants of foods (or drugs and cosmetics) has increased recently due to their reportedly high anti-oxidative, free radical scavenging activities and concerns about the use of various synthetic alternatives. The main commercial sources of betalains are powders and concentrates of red beet (Beta vulgaris) or cactus pear (Opuntia ficusindica) extracts. However, in recent years the technical and commercial feasibility of various in vitro systems to produce them biotechnologically has been explored. These research activities have included assessments of novel approaches for cultivating plant cell or tissue cultures, and diverse bioreactor systems for increasing production levels of secondary metabolites. This paper reviews recent progress in plant in vitro systems for producing betalain pigments. In addition, the factors that could be manipulated, the bioreactor systems that could be used, and the strategies that could be applied to improve betalain production are discussed.

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Betaxanthin formation and free amino acids in hairy roots of Beta vulgaris var. lutea depending on nutrient medium and glutamate or glutamine feeding

Cited by 24 publications

References 28 publications

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Betalains in the era of global agri-food science, technology and nutritional health

Betalain production in plant in vitro systems

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