Between-season Survival of the Grasshopper Pathogen Metarhizium flavoviride in the Sahel

Thomas, Bonnita A.; Gbongboui, C.; Lomer, C. J.

doi:10.1080/09583159631208

Cited by 23 publications

(8 citation statements)

References 10 publications

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“…Notwithstanding, bioassay has been the method of choice for detection of M. anisopliae var. acridum in field trials (Lomer et al 1993, Caudwell & Gatehouse 1996, Delgado et al 1997a, Langewald et al 1997, Lomer 1997, Magalha˜es et al 2000 and for surveys and screening of virulent isolates (Zimmerman et al 1994, Bateman et al 1996, Thomas, Gbongboui & Lomer 1996, Shah et al 1998.…”

Section: Introductionmentioning

confidence: 99%

Development of a PCR-based diagnostic assay for the specific detection of the entomopathogenic fungus Metarhizium anisopliae var. acridum

Entz

Johnson

Kawchuk

2005

Mycological Research

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Section: Introductionmentioning

confidence: 99%

Development of a PCR-based diagnostic assay for the specific detection of the entomopathogenic fungus Metarhizium anisopliae var. acridum

Entz

Johnson

Kawchuk

2005

Mycological Research

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“…Following an extensive development phase an isolate, IMI 330189, has been mass produced and commercialized under the product name`Green Muscle 0 ' [1]. This product has been shown to be a good alternative to organophosphate pesticides and carries the bene¢ts of speci¢city to acridid insects, compatibility with conventional ultra low volume spray application and long term e¡ect and persistence [2,3]. Another M. anisopliae var.…”

Section: Introductionmentioning

confidence: 99%

Biolistic co-transformation of Metarhizium anisopliae var. acridum strain CG423 with green fluorescent protein and resistance to glufosinate ammonium

Inglis

2000

FEMS Microbiology Letters

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Metarhizium anisopliae var. acridum (syn. M. flavoviride) is recognized as a highly specific and virulent mycopathogen of locusts and grasshoppers and is currently being developed as a biological control agent for this group of insects in Brazil. Intact conidia of M. anisopliae var. acridum strain CG423 were transformed using microparticle bombardment. Plasmids used were: (1) pBARKS1 carrying the bar gene of Streptomyces hygroscopicus fused to the Aspergillus nidulans trpC promoter, encoding resistance to glufosinate ammonium (or phosphinothricin) and modified by addition of the telomeric repeat (TTAGGG) 18 of Fusarium oxysporum and 2.pEGFP/gpd/tel carrying a red-shifted variant gene for Aequorea victoria green fluorescent protein (EGFP) which we have fused to the A. nidulans gpd promoter and trpC terminator. Highly fluorescent co-transformants were selected on solid minimal medium containing 100 Wg ml 31 glufosinate ammonium using an inverted microscope with 450^490 nm excitation/510 nm emission filter set. Southern blot analysis of co-transformants revealed varying multiple chromosomal integrations of both bar and egfp genes at both telomeric and non-telomeric loci. Transformants retained pathogenicity in bioassays against Rhammatocerus schistocercoides and showed unaltered lack of pathogenicity against larvae of the non-target insect Anticarsia gemmatalis. One co-transformant from four tested, however, showed a significant, but non-dose-dependent, elevation in virulence against Tenebrio molitor. ß

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“…Under natural conditions, M. anisopliae var. acridum probably recycles at a low level in susceptible host species, and is able to survive from one season to the next in favorable microhabitats, particularly in the cadavers of infected insects (Shah et al 1994, Thomas et al 1996.…”

Section: Selecting Biocontrol Agents In An Ipm Contextmentioning

confidence: 99%

What is the place of biological control in acridid integrated pest management?

Lomer¹,

Langewald

2001

Journal of Orthoptera Research

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Between-season Survival of the Grasshopper Pathogen Metarhizium flavoviride in the Sahel

Cited by 23 publications

References 10 publications

Development of a PCR-based diagnostic assay for the specific detection of the entomopathogenic fungus Metarhizium anisopliae var. acridum

Development of a PCR-based diagnostic assay for the specific detection of the entomopathogenic fungus Metarhizium anisopliae var. acridum

Biolistic co-transformation of Metarhizium anisopliae var. acridum strain CG423 with green fluorescent protein and resistance to glufosinate ammonium

What is the place of biological control in acridid integrated pest management?

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