Bevacizumab loaded solid lipid nanoparticles prepared by the coacervation technique: preliminaryin vitrostudies

Abstract

Glioblastoma, the most common primary brain tumor in adults, has an inauspicious prognosis, given that overcoming the blood-brain barrier is the major obstacle to the pharmacological treatment of brain tumors. As neoangiogenesis plays a key role in glioblastoma growth, the US Food and Drug Administration approved bevacizumab (BVZ), an antivascular endothelial growth factor antibody for the treatment of recurrent glioblastoma in patients whose the initial therapy has failed. In this experimental work, BVZ was entrapped in solid lipid nanoparticles (SLNs) prepared by the fatty-acid coacervation technique, thanks to the formation of a hydrophobic ion pair. BVZ activity, which was evaluated by means of four different in vitro tests on HUVEC cells, increased by 100- to 200-fold when delivered in SLNs. Moreover, SLNs can enhance the permeation of fluorescently labelled BVZ through an hCMEC/D3 cell monolayer-an in vitro model of the blood brain barrier. These results are promising, even if further in vivo studies are required to evaluate the effective potential of BVZ-loaded SLNs in glioblastoma treatment.

A new paradigm for antiangiogenic therapy through controlled release of bevacizumab from PLGA nanoparticles

“…retinal detachment, endophthalmitis, vitreous hemorrhage) 14 . Therefore, encapsulation of bevacizumab into nanocarriers holds the potential to overcome some of these drawbacks, increasing the half-time of bevacizumab, reducing the administration’s number and improving patients convenience 5, 15–18 . Additionally, NP may be targeted to specific cells and deliver the mAb inside the cell, when intracellular components are the target 10 .…”

Nanoparticles for Intravascular Applications: Physicochemical Characterization and Cytotoxicity Testing

“…In the recent years, increased numbers of publications appeared concerning the possible interactions between nanoparticles and endothelial cells, which are the first contact cells in the vascular wall for circulating nanoparticles. However, these reports usually focus on one type of nanosystems in the context of endothelial viability, or barrier function [6][7][8][9]. Thus, the purpose of this work was to perform comparative physicochemical and biological analyses of different types of nanoparticles intended for intravascular applications.…”

Synthesis and characterization of calcium-induced peanut protein isolate nanoparticles

“…Nowadays, many physic, chemical and biologic methods have been developed for the preparation of protein nanoparticle including desolvation, 20 hydrolysis, 21 pH-coacervation, 22 selfassembly 23 and emulsication dispersion. 24 Whereas, these methods still have some disadvantage with complicated process, sophisticated control and using of organic reagents.…”