Adapting to new cultures and understanding them differ in each individual. This difference is based on different personality traits. Personality traits affect success in communicating, active behaviours, relationship structures and the capability to perform. Cultural Intelligence is, in broad terms, described as the capability to effectively interact with individuals with different cultural backgrounds or the result of those interactions. Starting with the point that there is a relation between personality traits and Cultural Intelligence, it is aimed in this research to determine the effect of Five Factor Personality Traits on Cultural Intelligence. To this end, a research has been conducted by asking the opinions of Top Ranking Football Referees who referee matches within different cultures. The data gathered in the research have been evaluated with SPSS software package and findings have been obtained by testing the hypotheses. At the end of the research, it has been concluded that there are personality traits that affect the dimensions of Cultural Intelligence. Especially the result that the personality traits of openness to experience and conscientiousness affect all the dimensions of Cultural Intelligence is of great importance with regard to the results of the research.