Beyond molting-roles of the steroid molting hormone ecdysone in regulation of memory and sleep in adult Drosophila

Ishimoto, Hiroshi; Kitamoto, Toshihiro

doi:10.4161/fly.5.3.15477

Cited by 35 publications

(34 citation statements)

References 48 publications

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“…The discovery that male-transferred 20E is a key regulator of female reproductive biology in An. gambiae adds to the myriad of effects that this hormone exerts on insect adult physiology (2,3,(17)(18)(19)(20)(21)(22)(23). Interestingly, the steady decline of 20E levels in the atrium during the first day after copulation (17) is reminiscent of the process of mating plug digestion, which is completed in a similar time frame (5)(6)(7)16).…”

Section: Discussionmentioning

confidence: 99%

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Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Gabrieli

Kakani

Mitchell

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

112

174

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Significance Anopheles gambiae females are the principal vectors of malaria, a disease that kills more than 600,000 people every year. Current control methods using insecticides to kill mosquitoes are threatened by the spread of resistance in natural populations. A promising alternative control strategy is based on interfering with mosquito reproduction to reduce the number of malaria-transmitting females. Here we show that a male hormone transferred to the female during sex induces large changes in female behavior. These changes, defined as the postmating switch, include a physical incapacity for fertilization by additional males and the ability to lay mature eggs. Tampering with the function of this hormone generates unprecedented opportunities to reduce the reproductive success of Anopheles mosquitoes and impact malaria transmission.

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Section: Discussionmentioning

confidence: 99%

“…This hormone has been found to affect multiple aspects of adult insect physiology: It controls lifespan (19), learning (20), stress-induced responses (21), sleep regulation, and social interactions (22). Moreover 20E acts as a sexual hormone, influencing male sexual behavior in Drosophila (23).…”

Section: Significancementioning

confidence: 99%

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Gabrieli

Kakani

Mitchell

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

112

174

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“…Ecdysteroid can be detected in adult fly heads (Ishimoto and Kitamoto, 2010), and in addition to circadian behavior it regulates both sleep and memory processes (Ishimoto and Kitamoto, 2011). Our profiling results did not detect expression of genes encoding steroid (ecdysteroid) synthetic enzymes (the Halloween family of genes) in astrocytes (Table S1).…”

Section: Discussionmentioning

confidence: 99%

TRAP-seq Profiling and RNAi-Based Genetic Screens Identify Conserved Glial Genes Required for Adult Drosophila Behavior

Sengupta

Huang

et al. 2016

Front. Mol. Neurosci.

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Although, glial cells have well characterized functions in the developing and mature brain, it is only in the past decade that roles for these cells in behavior and plasticity have been delineated. Glial astrocytes and glia-neuron signaling, for example, are now known to have important modulatory functions in sleep, circadian behavior, memory and plasticity. To better understand mechanisms of glia-neuron signaling in the context of behavior, we have conducted cell-specific, genome-wide expression profiling of adult Drosophila astrocyte-like brain cells and performed RNA interference (RNAi)-based genetic screens to identify glial factors that regulate behavior. Importantly, our studies demonstrate that adult fly astrocyte-like cells and mouse astrocytes have similar molecular signatures; in contrast, fly astrocytes and surface glia—different classes of glial cells—have distinct expression profiles. Glial-specific expression of 653 RNAi constructs targeting 318 genes identified multiple factors associated with altered locomotor activity, circadian rhythmicity and/or responses to mechanical stress (bang sensitivity). Of interest, 1 of the relevant genes encodes a vesicle recycling factor, 4 encode secreted proteins and 3 encode membrane transporters. These results strongly support the idea that glia-neuron communication is vital for adult behavior.

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“…4G). The steroid hormone, ecdysone, induces significant developmental transitions and exerts pivotal biological functions in Drosophila , including molting, metamorphosis, oogenesis and wing development (Schubiger et al, 1998; Buszczak et al, 1999; Mirth et al, 2009), and even memory and sleep (Ishimoto and Kitamoto, 2011). The development of the Drosophila wing disc is a delicate and well-organized process involving many signaling pathways and factors, such as ecdysone, Notch and Hedgehog signaling, and Cut (Jack et al, 1991; de Celis and Garcia-Bellido, 1994; Mullor et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

The Ecdysone and Notch Pathways Synergistically Regulate Cut at the Dorsal–Ventral Boundary in Drosophila Wing Discs

Jia

Bryant

Jevitt

et al. 2016

Journal of Genetics and Genomics

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Metazoan development requires coordination of signaling pathways to regulate patterns of gene expression. In Drosophila, the wing imaginal disc provides an excellent model for the study of how signaling pathways interact to regulate pattern formation. The determination of the dorsal/ventral (DV) boundary of the wing disc depends on the Notch pathway, which is activated along the DV boundary and induces the expression of the homeobox transcription factor, Cut. Here, we show that Broad (Br), a zinc-finger transcription factor, is also involved in regulating Cut expression in the DV boundary region. However, Br expression is not regulated by Notch signaling in wing discs, rather, ecdysone signaling is the upstream signal that induces Br for Cut upregulation. Also, we find that the ecdysone-Br cascade upregulates cut-lacZ expression, a reporter containing a 2.7 kb cut enhancer region, implying that ecdysone signaling, similar to Notch, regulates cut at the transcriptional level. Collectively, our findings reveal that the Notch and ecdysone signaling pathways synergistically regulate Cut expression for proper DV boundary formation in the wing disc. Additionally, we show br promotes Delta, a Notch ligand, near the DV boundary to suppress aberrant high Notch activity, indicating further interaction between the two pathways for DV patterning of the wing disc.

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Beyond molting-roles of the steroid molting hormone ecdysone in regulation of memory and sleep in adult Drosophila

Cited by 35 publications

References 48 publications

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

TRAP-seq Profiling and RNAi-Based Genetic Screens Identify Conserved Glial Genes Required for Adult Drosophila Behavior

The Ecdysone and Notch Pathways Synergistically Regulate Cut at the Dorsal–Ventral Boundary in Drosophila Wing Discs

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