Beyond the Nanomaterials Approach: Influence of Culture Conditions on the Stability and Antimicrobial Activity of Silver Nanoparticles

Vázquez-Muñoz, Roberto; Bogdanchikova, Nina; Huerta-Saquero, Alejandro

doi:10.1021/acsomega.0c02007

Cited by 37 publications

(24 citation statements)

References 81 publications

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“…2 ). The stability of the nanoparticles as colloid is very important, as unstable NPs will not be able to disperse homogenously, which may effect on their antibacterial properties and reducing the efficacy 31 , 36 . Therefore, the polidyspersity index (PdI) was used as a value which show the stability of the nanomaterial.…”

Section: Resultsmentioning

confidence: 99%

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Skóra

Krajewska

Nowak

et al. 2021

Sci Rep

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Drug-resistance of bacteria is an ongoing problem in hospital treatment. The main mechanism of bacterial virulency in human infections is based on their adhesion ability and biofilm formation. Many approaches have been invented to overcome this problem, i.e. treatment with antibacterial biomolecules, which have some limitations e.g. enzymatic degradation and short shelf stability. Silver nanoparticles (AgNPs) may be alternative to these strategies due to their unique and high antibacterial properties. Herein, we report on yeast Saccharomyces cerevisiae extracellular-based synthesis of AgNPs. Transmission electron microscopy (TEM) revealed the morphology and structure of the metallic nanoparticles, which showed a uniform distribution and good colloid stability, measured by hydrodynamic light scattering (DLS). The energy dispersive X-ray spectroscopy (EDS) of NPs confirms the presence of silver and showed that sulfur-rich compounds act as a capping agent being adsorbed on the surface of AgNPs. Antimicrobial tests showed that AgNPs inhibit the bacteria growth, while have no impact on fungi growth. Moreover, tested NPs was characterized by high inhibitory potential of bacteria biofilm formation but also eradication of established biofilms. The cytotoxic effect of the NPs on four mammalian normal and cancer cell lines was tested through the metabolic activity, cell viability and wound-healing assays. Last, but not least, ability to deep penetration of the silver colloid to the root canal was imaged by scanning electron microscopy (SEM) to show its potential as the material for root-end filling.

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Section: Resultsmentioning

confidence: 99%

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Skóra

Krajewska

Nowak

et al. 2021

Sci Rep

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“…Only 0.2% of the silver contained in the Argovit™ formulation is released as ions. From a sample of 10 μg/mL AgNPs left under stirring for 24 h, 0.024 μg/mL of Ag + was quantified in solution by inductively coupled plasma atomic emission spectroscopy (ICP-OES) [ 55 ]. The larger stability of this formulation that avoids silver ion release is associated with the high amount of polyvinylpyrrolidone (PVP) used as a coating agent [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study

et al. 2021

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The use of nanomaterials is becoming increasingly widespread, leading to substantial research focused on nanomedicine. Nevertheless, the lack of complete toxicity profiles limits nanomaterials’ uses, despite their remarkable diagnostic and therapeutic results on in vitro and in vivo models. Silver nanoparticles (AgNPs), particularly Argovit™, have shown microbicidal, virucidal, and antitumoral effects. Among the first-line toxicity tests is the hemolysis assay. Here, the hemolytic effect of Argovit™ AgNPs on erythrocytes from one healthy donor (HDE) and one diabetic donor (DDE) is evaluated by the hemolysis assay against AgNO3. The results showed that Argovit™, in concentrations ≤24 µg/mL of metallic silver, did not show a hemolytic effect on the HDE or DDE. On the contrary, AgNO3 at the same concentration of silver ions produces more than 10% hemolysis in both the erythrocyte types. In all the experimental conditions assessed, the DDE was shown to be more prone to hemolysis than the HDE elicited by Ag+ ions or AgNPs, but much more evident with Ag+ ions. The results show that Argovit™ is the least hemolytic compared with the other twenty-two AgNP formulations previously reported, probably due to the polymer mass used to stabilize the Argovit™ formulation. The results obtained provide relevant information that contributes to obtaining a comprehensive toxicological profile to design safe and effective AgNP formulations.

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“…Based on our results, we have recommended to obtain a circumspect selection of the green extracts used for the synthesis of nanoparticles, and suggested that a comprehensive screen of the products should be carried out prior to their applications to delineate their behavior in the presence of living systems. Based on today’s nanotechnology results, combining the available methods of biology, chemistry and material sciences, more complex investigations can be carried out [ 162 , 163 ]. Using such an approach, systematic examinations regarding AgNP aggregation behavior with simultaneous measurements of its effect on biological activity can be performed to offer new frontiers to preserve nanoparticle toxicity by enhancing colloidal stability [ 70 ].…”

Section: Synthesismentioning

confidence: 99%

Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications

et al. 2021

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The nanomaterial industry generates gigantic quantities of metal-based nanomaterials for various technological and biomedical applications; however, concomitantly, it places a massive burden on the environment by utilizing toxic chemicals for the production process and leaving hazardous waste materials behind. Moreover, the employed, often unpleasant chemicals can affect the biocompatibility of the generated particles and severely restrict their application possibilities. On these grounds, green synthetic approaches have emerged, offering eco-friendly, sustainable, nature-derived alternative production methods, thus attenuating the ecological footprint of the nanomaterial industry. In the last decade, a plethora of biological materials has been tested to probe their suitability for nanomaterial synthesis. Although most of these approaches were successful, a large body of evidence indicates that the green material or entity used for the production would substantially define the physical and chemical properties and as a consequence, the biological activities of the obtained nanomaterials. The present review provides a comprehensive collection of the most recent green methodologies, surveys the major nanoparticle characterization techniques and screens the effects triggered by the obtained nanomaterials in various living systems to give an impression on the biomedical potential of green synthesized silver and gold nanoparticles.

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Beyond the Nanomaterials Approach: Influence of Culture Conditions on the Stability and Antimicrobial Activity of Silver Nanoparticles

Cited by 37 publications

References 81 publications

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study

Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications

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