Beziehungen zwischen zellständigen Lipiden und Dinatrium cromoglicicum

Abstract

Disodium cromoglycate binds in vitro and in vivo to lipids in white cells. Smears of cells from lymphocyte cultures and from bone marrow aspirates treated with DNCG and subsequently stained with pseudoisocyanine show a characteristic green fluorescence (515 nm) of membrane- and intracellular-lipids. It is suggested that the mode of action of DNCG in the prophylaxis of bronchial asthma could be the binding of DNCG to membrane lipids. This binding might block the IgE-mediated reaction on the surface of mast cells which otherwise would lead to degranulation and release of vasoactive substances.

Interaction of Disodium Cromoglycate with Calcium Ions in 2-Propanol

“…DSCG has been shown to react with magnesium ions to form a gel. but only at drug concentrations far in excess of those employed in pharmacologi cal experiments [6], So far these attempted reactions have all been carried out in aqueous solution, yet there is some evidence that DSCG asso ciates with the lipid components of cells [5]. It seemed possible, therefore, that a reaction between calcium and cromoglycate might be demonstrated in an appropriate organic sol vent.…”